Hyperthermophilic Archaeal Virus 2

Taxonomy: Viruses; unclassified archaeal viruses

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9CGC1|D9CGC1_9VIRU Uncharacterized protein OS=Hyperthermophilic Archaeal Virus 2 OX=762906 GN=HAV2_gp12 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84KK3 pKa = 8.83PVLLVLAVLVAGLASATLSSGQAAGVEE30 pKa = 4.53FNSVSIIVPGEE41 pKa = 3.79LLGSFQTYY49 pKa = 9.87LIPLNVSEE57 pKa = 4.71TGMLSTDD64 pKa = 3.2NVYY67 pKa = 10.93VIAQGVPRR75 pKa = 11.84FSYY78 pKa = 10.51LFSDD82 pKa = 4.4YY83 pKa = 10.39PPLVAVFEE91 pKa = 4.33RR92 pKa = 11.84SAYY95 pKa = 8.45NAVYY99 pKa = 9.12EE100 pKa = 4.15VWYY103 pKa = 10.71GGGNPYY109 pKa = 10.71SSFISVPGTGSSLWLVFDD127 pKa = 5.03DD128 pKa = 5.18FDD130 pKa = 4.95YY131 pKa = 10.83STGFWLVKK139 pKa = 9.84NASITGSRR147 pKa = 11.84VYY149 pKa = 10.72VDD151 pKa = 3.16PGGYY155 pKa = 9.54LALSAAYY162 pKa = 9.79SLRR165 pKa = 11.84TAHH168 pKa = 5.87VWLVHH173 pKa = 4.78GRR175 pKa = 11.84KK176 pKa = 9.53ALVISLQQEE185 pKa = 4.15YY186 pKa = 10.69SGFIALVFTTGNFTDD201 pKa = 3.63VSLVQNGSDD210 pKa = 3.4VFFIDD215 pKa = 4.2SLGNCLYY222 pKa = 10.94YY223 pKa = 10.91SLLHH227 pKa = 7.0LDD229 pKa = 3.52RR230 pKa = 11.84QAGIMIVVVNPAGNTVIYY248 pKa = 9.5MLYY251 pKa = 10.77GGLNKK256 pKa = 10.49CLDD259 pKa = 3.81YY260 pKa = 10.89RR261 pKa = 11.84VSS263 pKa = 3.2

Molecular weight: 28.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9CGC3|D9CGC3_9VIRU Uncharacterized protein OS=Hyperthermophilic Archaeal Virus 2 OX=762906 GN=HAV2_gp14 PE=4 SV=1
MM1 pKa = 7.5LACLAYY7 pKa = 10.48SSGVSGGVASEE18 pKa = 3.95AFIIIILPSSRR29 pKa = 11.84VTRR32 pKa = 11.84SLPLITPVLAFKK44 pKa = 10.45EE45 pKa = 4.44YY46 pKa = 10.48TSLPLYY52 pKa = 10.66LSIHH56 pKa = 5.75GFFVLVLNMCMSLEE70 pKa = 3.85QLIFLTVTKK79 pKa = 10.71GMVFVNGFTGGQLHH93 pKa = 6.47EE94 pKa = 4.02RR95 pKa = 11.84FIRR98 pKa = 11.84NKK100 pKa = 9.65VFDD103 pKa = 5.48HH104 pKa = 6.34IPPRR108 pKa = 11.84AVQSRR113 pKa = 11.84LDD115 pKa = 3.37IPEE118 pKa = 4.12SFKK121 pKa = 11.23KK122 pKa = 10.46PLVALPKK129 pKa = 10.29VYY131 pKa = 10.59LNEE134 pKa = 4.03HH135 pKa = 6.15RR136 pKa = 11.84HH137 pKa = 4.94LHH139 pKa = 5.88PLFPSPLTSAPSGLTLSIRR158 pKa = 11.84SRR160 pKa = 11.84ILRR163 pKa = 11.84NSLDD167 pKa = 3.58INTSTASAIILNAWAEE183 pKa = 4.16MGFRR187 pKa = 11.84PCSS190 pKa = 3.31

Molecular weight: 20.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

5384

93

1767

358.9

39.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.689 ± 0.244

0.743 ± 0.174

4.123 ± 0.234

5.665 ± 1.276

3.529 ± 0.258

7.207 ± 0.544

1.449 ± 0.273

5.609 ± 0.466

4.941 ± 1.122

10.048 ± 0.512

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.746 ± 0.264

3.845 ± 0.528

5.126 ± 0.209

2.025 ± 0.172

5.479 ± 0.631

8.042 ± 0.901

5.981 ± 0.967

9.733 ± 0.667

1.597 ± 0.124

5.405 ± 0.747
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski