Slackia piriformis YIT 12062

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Slackia; Slackia piriformis

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0YIV2|K0YIV2_9ACTN HD domain-containing protein OS=Slackia piriformis YIT 12062 OX=742818 GN=HMPREF9451_01007 PE=4 SV=1
MM1 pKa = 7.54EE2 pKa = 5.0EE3 pKa = 4.39KK4 pKa = 10.22KK5 pKa = 11.04GPAAPQPAPDD15 pKa = 4.25NQPKK19 pKa = 10.31SDD21 pKa = 3.46ISAEE25 pKa = 4.07PAAPAAPVEE34 pKa = 4.63PPAPTAPAAAPAEE47 pKa = 4.19PAAPAEE53 pKa = 4.26PAAPAEE59 pKa = 4.2PAVPAEE65 pKa = 4.18PAAPAEE71 pKa = 4.5PAAPMAPVAPTAPAAPQAAPASAPQVEE98 pKa = 5.16GTAQMPQTPHH108 pKa = 6.22QPVSAPTQPFDD119 pKa = 4.47SAQQPAQPVPGPAQAPYY136 pKa = 10.34GAYY139 pKa = 9.33PPPPAPNYY147 pKa = 8.93SAPSSGKK154 pKa = 8.5ATGALICGILAIVFSFIPIVGIILGIVAIVLASKK188 pKa = 10.26AVKK191 pKa = 9.79FAGKK195 pKa = 9.76SGKK198 pKa = 6.23TTGGKK203 pKa = 8.74VCGIIGIVFSILWAIFSFVLTMGIIGGINAYY234 pKa = 9.84EE235 pKa = 4.1SDD237 pKa = 4.55YY238 pKa = 11.33VPSADD243 pKa = 3.93VPTTSSPLAEE253 pKa = 4.47DD254 pKa = 3.27MGAEE258 pKa = 4.06EE259 pKa = 4.4QAIADD264 pKa = 4.18LVSAEE269 pKa = 3.72LDD271 pKa = 3.32KK272 pKa = 11.72YY273 pKa = 11.35KK274 pKa = 10.96NADD277 pKa = 3.48SAALSALGAEE287 pKa = 4.74YY288 pKa = 10.6DD289 pKa = 4.56DD290 pKa = 4.95EE291 pKa = 5.65DD292 pKa = 4.68LVTIDD297 pKa = 4.37GVDD300 pKa = 3.3MSLSDD305 pKa = 5.35LGIDD309 pKa = 3.88PNEE312 pKa = 3.81VAAWATTDD320 pKa = 3.59FDD322 pKa = 4.48YY323 pKa = 11.4SIDD326 pKa = 3.64SVYY329 pKa = 10.43IDD331 pKa = 4.14SDD333 pKa = 3.8GATATVYY340 pKa = 11.03VDD342 pKa = 3.05TTEE345 pKa = 4.49RR346 pKa = 11.84DD347 pKa = 3.63FFEE350 pKa = 4.41MGSLFGDD357 pKa = 4.3LLNEE361 pKa = 4.23YY362 pKa = 9.36VSSGAADD369 pKa = 3.53AADD372 pKa = 4.62LEE374 pKa = 4.57TAKK377 pKa = 11.03AKK379 pKa = 10.7VGEE382 pKa = 4.63LYY384 pKa = 10.41AQAMAEE390 pKa = 4.16STAMSDD396 pKa = 3.25YY397 pKa = 10.72YY398 pKa = 11.46AAFDD402 pKa = 3.57IVNEE406 pKa = 4.3GGTWRR411 pKa = 11.84ILEE414 pKa = 4.46DD415 pKa = 3.62SLEE418 pKa = 4.15EE419 pKa = 3.78EE420 pKa = 4.04LDD422 pKa = 3.92YY423 pKa = 11.68VFSTFF428 pKa = 4.33

Molecular weight: 43.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0YL01|K0YL01_9ACTN Uncharacterized protein OS=Slackia piriformis YIT 12062 OX=742818 GN=HMPREF9451_00882 PE=4 SV=1
MM1 pKa = 7.18EE2 pKa = 5.86RR3 pKa = 11.84KK4 pKa = 9.19PVDD7 pKa = 3.48EE8 pKa = 4.45YY9 pKa = 11.62LATGPVEE16 pKa = 3.96EE17 pKa = 4.56SSFRR21 pKa = 11.84SVILFGRR28 pKa = 11.84NTASYY33 pKa = 10.74KK34 pKa = 10.3FALAKK39 pKa = 10.68ALFDD43 pKa = 4.28LAGQNRR49 pKa = 11.84DD50 pKa = 3.44SVTLNQLAVPYY61 pKa = 8.55TKK63 pKa = 10.44HH64 pKa = 6.37LVEE67 pKa = 4.8HH68 pKa = 6.83AGSNPRR74 pKa = 11.84QTTSRR79 pKa = 11.84SSRR82 pKa = 11.84FMDD85 pKa = 3.48ACVGYY90 pKa = 10.83AKK92 pKa = 10.49GSEE95 pKa = 4.03IYY97 pKa = 10.29DD98 pKa = 3.52RR99 pKa = 11.84LISAVVDD106 pKa = 3.87LGFDD110 pKa = 3.31NVLDD114 pKa = 4.18AFHH117 pKa = 6.54TVGKK121 pKa = 10.31GDD123 pKa = 3.47VPVRR127 pKa = 11.84FSKK130 pKa = 10.79RR131 pKa = 11.84IFRR134 pKa = 11.84GDD136 pKa = 3.42RR137 pKa = 11.84NVSSLPTRR145 pKa = 11.84SSNWRR150 pKa = 11.84IRR152 pKa = 11.84PRR154 pKa = 11.84RR155 pKa = 11.84PASFKK160 pKa = 10.58KK161 pKa = 10.39SNRR164 pKa = 11.84DD165 pKa = 3.3GASSRR170 pKa = 11.84PPGRR174 pKa = 11.84PASALAFFPMMEE186 pKa = 4.31RR187 pKa = 11.84PGGSCLMAVCAEE199 pKa = 3.94GTLRR203 pKa = 11.84RR204 pKa = 11.84RR205 pKa = 11.84ASRR208 pKa = 3.31

Molecular weight: 23.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1794

0

1794

600490

22

1949

334.7

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.322 ± 0.068

1.74 ± 0.028

5.936 ± 0.048

6.906 ± 0.065

4.089 ± 0.038

8.03 ± 0.053

1.861 ± 0.024

5.313 ± 0.047

4.176 ± 0.043

8.928 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.024

2.917 ± 0.031

4.07 ± 0.031

2.78 ± 0.03

5.77 ± 0.069

6.408 ± 0.048

4.886 ± 0.041

8.139 ± 0.053

1.016 ± 0.021

2.83 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski