Brucella phage BiPBO1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141GEY8|A0A141GEY8_9CAUD Acyltransferase OS=Brucella phage BiPBO1 OX=1718278 GN=BiPBO1_32 PE=4 SV=1
MM1 pKa = 7.21MSSEE5 pKa = 4.12IPSVGTAVFWATDD18 pKa = 3.48DD19 pKa = 4.6GIARR23 pKa = 11.84GVVQSHH29 pKa = 6.26EE30 pKa = 4.53SDD32 pKa = 3.98CEE34 pKa = 4.07TGEE37 pKa = 4.06FRR39 pKa = 11.84CLKK42 pKa = 10.23IEE44 pKa = 3.81SLYY47 pKa = 10.29YY48 pKa = 10.14RR49 pKa = 11.84RR50 pKa = 11.84GGQDD54 pKa = 2.32IGSPIGTTFLAMPSDD69 pKa = 3.99VLLEE73 pKa = 4.19EE74 pKa = 4.95PNN76 pKa = 3.36

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141GEW7|A0A141GEW7_9CAUD Uncharacterized protein OS=Brucella phage BiPBO1 OX=1718278 GN=BiPBO1_11 PE=4 SV=1
MM1 pKa = 8.14VEE3 pKa = 3.91GLDD6 pKa = 3.4RR7 pKa = 11.84LKK9 pKa = 10.98RR10 pKa = 11.84KK11 pKa = 7.73LTKK14 pKa = 10.03TIPTAVVGATVKK26 pKa = 10.8AMEE29 pKa = 4.08QGADD33 pKa = 3.28EE34 pKa = 4.73VVSMMRR40 pKa = 11.84RR41 pKa = 11.84LVPKK45 pKa = 9.68DD46 pKa = 3.3TGKK49 pKa = 10.53LAATINWTWGEE60 pKa = 4.01APEE63 pKa = 4.96GSLTLGRR70 pKa = 11.84SKK72 pKa = 9.59TAANGLRR79 pKa = 11.84ITIYY83 pKa = 10.62AGDD86 pKa = 3.97MSTMVGEE93 pKa = 4.62RR94 pKa = 11.84SQFQLARR101 pKa = 11.84LQEE104 pKa = 4.45FGTQHH109 pKa = 5.3MQANPYY115 pKa = 9.15FFPSWRR121 pKa = 11.84ASRR124 pKa = 11.84KK125 pKa = 8.21RR126 pKa = 11.84VKK128 pKa = 10.69GRR130 pKa = 11.84ITRR133 pKa = 11.84QMRR136 pKa = 11.84KK137 pKa = 9.63AIKK140 pKa = 10.39DD141 pKa = 3.38GAKK144 pKa = 10.2

Molecular weight: 16.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

14141

40

861

164.4

18.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.954 ± 0.516

0.933 ± 0.107

6.117 ± 0.226

6.485 ± 0.244

3.564 ± 0.181

7.616 ± 0.258

1.867 ± 0.16

6.004 ± 0.181

5.509 ± 0.276

7.963 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.914 ± 0.166

3.713 ± 0.237

4.201 ± 0.246

3.642 ± 0.219

6.52 ± 0.261

5.848 ± 0.246

5.636 ± 0.233

6.449 ± 0.208

1.591 ± 0.121

2.475 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski