Nerine latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I2E5K8|I2E5K8_9VIRU Capsid protein OS=Nerine latent virus OX=797075 GN=CP PE=3 SV=1
MM1 pKa = 7.28FPKK4 pKa = 10.62LSASFYY10 pKa = 9.69LTTILTSVLTFLLFSASQPNEE31 pKa = 3.92NKK33 pKa = 8.91CTVIITGEE41 pKa = 4.1SIRR44 pKa = 11.84ILGCTFSEE52 pKa = 5.16GFLEE56 pKa = 4.37YY57 pKa = 10.91AKK59 pKa = 10.91GLDD62 pKa = 3.52VLRR65 pKa = 11.84VV66 pKa = 3.37

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I2E5K9|I2E5K9_9VIRU Nucleotide binding protein OS=Nerine latent virus OX=797075 GN=NTBP PE=4 SV=1
MM1 pKa = 7.27KK2 pKa = 9.5TLCYY6 pKa = 10.11KK7 pKa = 9.95DD8 pKa = 3.57TLNTVALCLYY18 pKa = 8.93RR19 pKa = 11.84LCVPLDD25 pKa = 3.95IIYY28 pKa = 10.19LIQGKK33 pKa = 10.04VYY35 pKa = 9.98TNTRR39 pKa = 11.84LYY41 pKa = 10.34EE42 pKa = 3.92VARR45 pKa = 11.84QSSITPFSGTSRR57 pKa = 11.84SAIKK61 pKa = 10.07RR62 pKa = 11.84RR63 pKa = 11.84AMRR66 pKa = 11.84EE67 pKa = 3.66NRR69 pKa = 11.84CYY71 pKa = 10.72KK72 pKa = 10.14CGKK75 pKa = 9.65DD76 pKa = 3.2FHH78 pKa = 7.19KK79 pKa = 10.81YY80 pKa = 8.85KK81 pKa = 10.5CRR83 pKa = 11.84SNTTSQQEE91 pKa = 3.8FVEE94 pKa = 4.36YY95 pKa = 10.18FKK97 pKa = 11.06EE98 pKa = 4.65GPTRR102 pKa = 11.84FDD104 pKa = 3.24TEE106 pKa = 4.04KK107 pKa = 10.97ARR109 pKa = 11.84TNSMIILYY117 pKa = 9.64HH118 pKa = 5.84EE119 pKa = 4.51RR120 pKa = 11.84QLDD123 pKa = 3.36RR124 pKa = 11.84MEE126 pKa = 3.6FHH128 pKa = 6.68INKK131 pKa = 9.64RR132 pKa = 11.84PSTT135 pKa = 3.82

Molecular weight: 16.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2718

66

1873

453.0

51.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.482 ± 0.775

2.502 ± 0.393

5.592 ± 0.617

7.616 ± 1.049

6.328 ± 0.868

5.151 ± 0.488

1.729 ± 0.483

6.99 ± 0.409

8.241 ± 1.174

9.198 ± 0.864

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.418

4.82 ± 0.424

3.569 ± 0.932

2.87 ± 0.452

5.519 ± 0.977

7.211 ± 0.398

5.335 ± 1.442

4.599 ± 0.351

0.662 ± 0.211

3.826 ± 0.506
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski