Campylobacter phage vB_CjeM_Los1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Eucampyvirinae; Fletchervirus; Campylobacter virus Los1

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8EXD0|A0A1D8EXD0_9CAUD Uncharacterized protein OS=Campylobacter phage vB_CjeM_Los1 OX=1904491 GN=LOS1_00045 PE=4 SV=1
MM1 pKa = 7.36YY2 pKa = 9.76YY3 pKa = 10.81SNVVFPLNEE12 pKa = 4.13VIPGFTYY19 pKa = 10.44NVKK22 pKa = 10.03KK23 pKa = 10.76SKK25 pKa = 10.42SNVYY29 pKa = 9.9IRR31 pKa = 11.84FVAYY35 pKa = 9.81GYY37 pKa = 10.87SIDD40 pKa = 4.63DD41 pKa = 4.25LEE43 pKa = 4.2IVYY46 pKa = 10.4NNSIITISTIKK57 pKa = 10.33DD58 pKa = 3.3YY59 pKa = 11.64HH60 pKa = 5.84EE61 pKa = 4.57VKK63 pKa = 10.11TDD65 pKa = 3.51PKK67 pKa = 10.53FSNNFPQQDD76 pKa = 3.06KK77 pKa = 10.75FYY79 pKa = 10.64IQFWCPKK86 pKa = 8.8ISGINAEE93 pKa = 4.08YY94 pKa = 10.43SGNFIKK100 pKa = 10.81LNCSLGDD107 pKa = 3.2ISVNLGVIPIKK118 pKa = 10.46FINEE122 pKa = 4.01DD123 pKa = 3.34NDD125 pKa = 3.25VDD127 pKa = 3.82ILEE130 pKa = 4.5NTSDD134 pKa = 3.45DD135 pKa = 3.62TMNIIQLNGFMDD147 pKa = 4.11EE148 pKa = 4.74LEE150 pKa = 4.68GIKK153 pKa = 10.52DD154 pKa = 3.64DD155 pKa = 4.52FYY157 pKa = 12.02SEE159 pKa = 3.69ITNNKK164 pKa = 9.04DD165 pKa = 2.74

Molecular weight: 19.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8EX80|A0A1D8EX80_9CAUD Uncharacterized protein OS=Campylobacter phage vB_CjeM_Los1 OX=1904491 GN=LOS1_00039 PE=4 SV=1
MM1 pKa = 7.69PPIKK5 pKa = 10.28HH6 pKa = 5.99MSVADD11 pKa = 5.29RR12 pKa = 11.84IAQKK16 pKa = 10.37RR17 pKa = 11.84YY18 pKa = 9.02RR19 pKa = 11.84KK20 pKa = 8.84QPKK23 pKa = 9.0VKK25 pKa = 10.09RR26 pKa = 11.84KK27 pKa = 9.97LKK29 pKa = 10.22IRR31 pKa = 11.84AKK33 pKa = 10.15KK34 pKa = 8.42NAKK37 pKa = 9.87APSEE41 pKa = 3.99NMSWSSKK48 pKa = 8.72KK49 pKa = 9.93RR50 pKa = 11.84GYY52 pKa = 8.83VRR54 pKa = 11.84KK55 pKa = 9.87DD56 pKa = 2.95PKK58 pKa = 10.58LRR60 pKa = 11.84RR61 pKa = 11.84TMKK64 pKa = 10.55LVAKK68 pKa = 10.31LRR70 pKa = 11.84RR71 pKa = 11.84KK72 pKa = 9.51SS73 pKa = 3.27

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

169

0

169

41923

35

1264

248.1

28.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.332 ± 0.175

1.818 ± 0.176

6.312 ± 0.213

6.796 ± 0.168

5.019 ± 0.13

4.976 ± 0.167

1.598 ± 0.122

9.303 ± 0.225

10.176 ± 0.239

8.68 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.113 ± 0.082

8.623 ± 0.16

2.617 ± 0.092

2.664 ± 0.085

2.624 ± 0.096

6.898 ± 0.173

5.2 ± 0.165

5.14 ± 0.161

0.809 ± 0.049

5.303 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski