Campylobacter sp. MIT 12-5580

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter; unclassified Campylobacter

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1777 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4U7BFL7|A0A4U7BFL7_9PROT TonB-dependent receptor OS=Campylobacter sp. MIT 12-5580 OX=2040651 GN=CQA38_07235 PE=4 SV=1
MM1 pKa = 8.04RR2 pKa = 11.84EE3 pKa = 3.76FEE5 pKa = 4.68DD6 pKa = 6.45DD7 pKa = 3.69EE8 pKa = 6.27FEE10 pKa = 5.82DD11 pKa = 6.44LDD13 pKa = 4.21DD14 pKa = 5.14QDD16 pKa = 3.48ILSYY20 pKa = 10.95EE21 pKa = 4.47DD22 pKa = 3.42EE23 pKa = 4.88DD24 pKa = 4.99DD25 pKa = 3.98YY26 pKa = 11.89LRR28 pKa = 11.84SEE30 pKa = 4.01HH31 pKa = 6.22SRR33 pKa = 11.84NYY35 pKa = 10.93NYY37 pKa = 10.92DD38 pKa = 3.31EE39 pKa = 5.53DD40 pKa = 5.61DD41 pKa = 4.02YY42 pKa = 12.11NFDD45 pKa = 5.17DD46 pKa = 4.49EE47 pKa = 6.56DD48 pKa = 5.43YY49 pKa = 11.11DD50 pKa = 4.71DD51 pKa = 4.6SYY53 pKa = 12.23EE54 pKa = 4.06MEE56 pKa = 4.11

Molecular weight:
7.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4U7BD90|A0A4U7BD90_9PROT Uncharacterized protein OS=Campylobacter sp. MIT 12-5580 OX=2040651 GN=CQA38_05020 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.06QPHH8 pKa = 5.76KK9 pKa = 7.92TPKK12 pKa = 9.96KK13 pKa = 7.47RR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.07TKK25 pKa = 10.39NGRR28 pKa = 11.84KK29 pKa = 9.3VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight:
5.29 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1777

0

1777

557218

37

1944

313.6

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.602 ± 0.06

1.197 ± 0.022

5.069 ± 0.037

7.115 ± 0.068

6.163 ± 0.061

5.533 ± 0.066

1.726 ± 0.022

7.664 ± 0.055

9.234 ± 0.075

11.365 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.024

5.531 ± 0.056

2.615 ± 0.032

3.559 ± 0.039

3.162 ± 0.035

6.866 ± 0.05

4.102 ± 0.048

5.263 ± 0.051

0.624 ± 0.015

3.541 ± 0.038

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski