Mycobacterium phage Roary

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9L602|A0A6B9L602_9CAUD Uncharacterized protein OS=Mycobacterium phage Roary OX=2686237 GN=50 PE=4 SV=1
MM1 pKa = 7.24ARR3 pKa = 11.84IYY5 pKa = 10.83VADD8 pKa = 4.32LAAYY12 pKa = 8.91NAGVLHH18 pKa = 6.5GEE20 pKa = 4.15WIDD23 pKa = 3.77LNGRR27 pKa = 11.84DD28 pKa = 4.51SEE30 pKa = 4.57EE31 pKa = 3.44VWAEE35 pKa = 4.1VKK37 pKa = 10.92AMLAEE42 pKa = 4.34GQKK45 pKa = 10.79LYY47 pKa = 11.0VSKK50 pKa = 9.52TLGVHH55 pKa = 5.61EE56 pKa = 4.84EE57 pKa = 4.16WAIHH61 pKa = 6.54DD62 pKa = 4.03YY63 pKa = 11.45EE64 pKa = 4.98GFGSLRR70 pKa = 11.84IGEE73 pKa = 4.36YY74 pKa = 10.84DD75 pKa = 6.09DD76 pKa = 4.53IDD78 pKa = 4.05QLIDD82 pKa = 3.85LADD85 pKa = 3.83ALEE88 pKa = 4.11KK89 pKa = 10.75HH90 pKa = 6.09GEE92 pKa = 4.04PFEE95 pKa = 4.38AWLSEE100 pKa = 3.95IIGDD104 pKa = 3.3IGYY107 pKa = 8.55FASVSDD113 pKa = 5.38AEE115 pKa = 4.55DD116 pKa = 3.24QFEE119 pKa = 4.47DD120 pKa = 3.95RR121 pKa = 11.84FIGAVTKK128 pKa = 10.56EE129 pKa = 4.04DD130 pKa = 3.52YY131 pKa = 10.86AYY133 pKa = 10.52DD134 pKa = 3.74YY135 pKa = 11.85AEE137 pKa = 5.11DD138 pKa = 3.84VLGLTGVALDD148 pKa = 3.63YY149 pKa = 11.09FDD151 pKa = 4.31YY152 pKa = 11.37EE153 pKa = 4.1KK154 pKa = 10.53FARR157 pKa = 11.84DD158 pKa = 3.55LEE160 pKa = 4.29MSGDD164 pKa = 3.51ISEE167 pKa = 4.95CYY169 pKa = 10.48YY170 pKa = 11.28NGTDD174 pKa = 3.33YY175 pKa = 11.44LFRR178 pKa = 11.84SWW180 pKa = 4.0

Molecular weight: 20.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LC09|A0A6B9LC09_9CAUD Minor tail protein OS=Mycobacterium phage Roary OX=2686237 GN=5 PE=4 SV=1
MM1 pKa = 7.4SWLTSARR8 pKa = 11.84RR9 pKa = 11.84FDD11 pKa = 5.54LPPDD15 pKa = 3.27WEE17 pKa = 4.24VRR19 pKa = 11.84RR20 pKa = 11.84QEE22 pKa = 4.38VLDD25 pKa = 4.3DD26 pKa = 4.24ANGVCEE32 pKa = 4.31IRR34 pKa = 11.84GPGCLGWATEE44 pKa = 3.88VDD46 pKa = 4.01HH47 pKa = 7.09IRR49 pKa = 11.84RR50 pKa = 11.84GNDD53 pKa = 2.65HH54 pKa = 6.47SRR56 pKa = 11.84RR57 pKa = 11.84NLQAACSKK65 pKa = 10.62CHH67 pKa = 5.76GKK69 pKa = 10.3KK70 pKa = 10.37SSAEE74 pKa = 3.6GNARR78 pKa = 11.84KK79 pKa = 9.28RR80 pKa = 11.84QLRR83 pKa = 11.84AMRR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84PPDD91 pKa = 3.2RR92 pKa = 11.84HH93 pKa = 6.14PGSMM97 pKa = 3.91

Molecular weight: 11.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

16396

32

1025

164.0

18.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.374 ± 0.427

0.909 ± 0.115

6.959 ± 0.217

7.423 ± 0.307

3.391 ± 0.175

8.13 ± 0.287

1.939 ± 0.136

4.94 ± 0.185

4.654 ± 0.327

8.459 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.159

3.312 ± 0.199

5.221 ± 0.274

3.397 ± 0.174

5.77 ± 0.33

5.276 ± 0.191

6.172 ± 0.254

6.752 ± 0.197

2.226 ± 0.17

3.123 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski