Marinobacterium aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Marinobacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4388 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A9EW30|A0A1A9EW30_9GAMM Anthranilate synthase component 2 OS=Marinobacterium aestuarii OX=1821621 GN=A8C75_04660 PE=4 SV=1
MM1 pKa = 7.24NAIGVQWSGEE11 pKa = 3.83EE12 pKa = 3.91LLFDD16 pKa = 4.39FSQMEE21 pKa = 4.08QLLTAEE27 pKa = 5.03LSLDD31 pKa = 3.59SEE33 pKa = 4.59KK34 pKa = 11.27VYY36 pKa = 11.11DD37 pKa = 4.36AFDD40 pKa = 3.87LATVLDD46 pKa = 4.44NNSSSWSLRR55 pKa = 11.84DD56 pKa = 3.53FVQASIDD63 pKa = 3.76TLPSLGVLGDD73 pKa = 3.74TPFDD77 pKa = 3.51NEE79 pKa = 4.09RR80 pKa = 11.84YY81 pKa = 9.44IAGTLTAEE89 pKa = 4.48TLTGTASDD97 pKa = 3.7NFMLAGAGDD106 pKa = 4.85DD107 pKa = 3.86IVNTGAGNDD116 pKa = 3.49EE117 pKa = 4.41VFAGTGNDD125 pKa = 3.23VVTASHH131 pKa = 6.34SGSNYY136 pKa = 9.94LHH138 pKa = 6.8GGSDD142 pKa = 3.95DD143 pKa = 3.95DD144 pKa = 4.21LLRR147 pKa = 11.84VARR150 pKa = 11.84TDD152 pKa = 3.11TYY154 pKa = 11.19RR155 pKa = 11.84SYY157 pKa = 11.35NAQTAAGNSNALIGGEE173 pKa = 4.18GNDD176 pKa = 4.5RR177 pKa = 11.84LEE179 pKa = 4.25GWTGSDD185 pKa = 3.0TYY187 pKa = 11.51VFNRR191 pKa = 11.84GDD193 pKa = 3.7GQDD196 pKa = 3.39VVSDD200 pKa = 4.16YY201 pKa = 11.78DD202 pKa = 2.57WGYY205 pKa = 10.72QVGIYY210 pKa = 9.08YY211 pKa = 10.54SRR213 pKa = 11.84LFYY216 pKa = 10.88SGTVDD221 pKa = 3.09QVLSVAVV228 pKa = 3.56

Molecular weight: 24.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A9F469|A0A1A9F469_9GAMM Uncharacterized protein OS=Marinobacterium aestuarii OX=1821621 GN=A8C75_06310 PE=4 SV=1
MM1 pKa = 8.38DD2 pKa = 4.65IGEE5 pKa = 4.24VAKK8 pKa = 10.95LSGVPASTLRR18 pKa = 11.84YY19 pKa = 9.72YY20 pKa = 10.51EE21 pKa = 4.27DD22 pKa = 3.38KK23 pKa = 11.38GLIRR27 pKa = 11.84SVGRR31 pKa = 11.84RR32 pKa = 11.84GLRR35 pKa = 11.84RR36 pKa = 11.84LFGGGVLEE44 pKa = 4.08RR45 pKa = 11.84LALIALGRR53 pKa = 11.84SAGFSLDD60 pKa = 4.08EE61 pKa = 4.02IAGMLGTGAEE71 pKa = 3.9PRR73 pKa = 11.84IDD75 pKa = 3.64RR76 pKa = 11.84RR77 pKa = 11.84QLNAKK82 pKa = 9.92ADD84 pKa = 3.97EE85 pKa = 4.53LDD87 pKa = 3.45VTIRR91 pKa = 11.84RR92 pKa = 11.84LTAMRR97 pKa = 11.84EE98 pKa = 4.32GLRR101 pKa = 11.84HH102 pKa = 5.54VADD105 pKa = 4.94CSAPGHH111 pKa = 5.79LEE113 pKa = 3.72CPRR116 pKa = 11.84FRR118 pKa = 11.84RR119 pKa = 11.84LMEE122 pKa = 4.26LAAAGALGDD131 pKa = 3.87GRR133 pKa = 11.84DD134 pKa = 3.86GRR136 pKa = 11.84KK137 pKa = 9.72APAPRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84ADD146 pKa = 3.53PPAVTT151 pKa = 4.48

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4388

0

4388

1459467

56

6150

332.6

36.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.592 ± 0.039

1.117 ± 0.013

5.601 ± 0.047

5.735 ± 0.035

3.72 ± 0.023

7.884 ± 0.044

2.186 ± 0.02

5.315 ± 0.026

3.209 ± 0.032

11.758 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.02

3.017 ± 0.026

4.595 ± 0.025

4.646 ± 0.028

6.32 ± 0.044

6.075 ± 0.036

4.828 ± 0.036

6.94 ± 0.032

1.331 ± 0.016

2.618 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski