Vibrio rumoiensis 1S-45

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio rumoiensis

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2981 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5E189|A0A1E5E189_9VIBR Flavodoxin OS=Vibrio rumoiensis 1S-45 OX=1188252 GN=A1QC_02380 PE=3 SV=1
MM1 pKa = 7.65KK2 pKa = 9.08KK3 pKa = 8.28TLLALAVSTIAFAGSVNAAEE23 pKa = 5.33LYY25 pKa = 10.92SSDD28 pKa = 3.42TTSVKK33 pKa = 10.68LSGEE37 pKa = 3.58VDD39 pKa = 3.54TYY41 pKa = 11.76LKK43 pKa = 10.29TGDD46 pKa = 4.51LKK48 pKa = 11.52FEE50 pKa = 4.34GQPKK54 pKa = 10.33DD55 pKa = 3.66KK56 pKa = 10.66SDD58 pKa = 3.62PNAAIWAKK66 pKa = 8.0TQFDD70 pKa = 3.74VDD72 pKa = 4.01YY73 pKa = 10.82KK74 pKa = 10.93ISTNNTAFLSFEE86 pKa = 4.2VEE88 pKa = 3.97GDD90 pKa = 3.11GDD92 pKa = 3.87AGAKK96 pKa = 9.96FDD98 pKa = 4.59DD99 pKa = 4.75VYY101 pKa = 11.78AGFTNNTFGTFTIGEE116 pKa = 4.37AGDD119 pKa = 3.89SFDD122 pKa = 5.31ALEE125 pKa = 4.64KK126 pKa = 10.43TDD128 pKa = 3.41ITNEE132 pKa = 4.07GIYY135 pKa = 10.46AGTIEE140 pKa = 5.76SEE142 pKa = 4.32DD143 pKa = 3.32SSNAIRR149 pKa = 11.84WQKK152 pKa = 11.14AFGGLALSADD162 pKa = 3.8AQTNTDD168 pKa = 3.17SDD170 pKa = 4.59DD171 pKa = 3.59NVYY174 pKa = 10.95ALSGDD179 pKa = 3.44WTITDD184 pKa = 3.99AFSVGAGYY192 pKa = 7.95ATSGSDD198 pKa = 3.47AYY200 pKa = 8.22TTALSASAQFGDD212 pKa = 4.61LYY214 pKa = 11.04LAASASQYY222 pKa = 11.41KK223 pKa = 10.47NFGGDD228 pKa = 3.46FEE230 pKa = 4.8FTGEE234 pKa = 4.13TEE236 pKa = 3.75KK237 pKa = 10.67TFNVDD242 pKa = 2.39ITEE245 pKa = 4.32GNTYY249 pKa = 10.48GIAAAYY255 pKa = 9.23QMNEE259 pKa = 3.23TRR261 pKa = 11.84FYY263 pKa = 11.32GSYY266 pKa = 11.21ALVDD270 pKa = 4.36DD271 pKa = 4.57DD272 pKa = 5.1TDD274 pKa = 3.79VKK276 pKa = 10.93FNAYY280 pKa = 8.07TVGIDD285 pKa = 3.71HH286 pKa = 7.26SIIDD290 pKa = 3.7NVLVFVEE297 pKa = 4.37YY298 pKa = 10.34TGVTGDD304 pKa = 4.02DD305 pKa = 3.42TTGNDD310 pKa = 3.42TTGNLYY316 pKa = 9.79MLGAYY321 pKa = 7.66LTFF324 pKa = 4.85

Molecular weight: 34.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5E338|A0A1E5E338_9VIBR Methylated-DNA--protein-cysteine methyltransferase OS=Vibrio rumoiensis 1S-45 OX=1188252 GN=A1QC_08280 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.5RR3 pKa = 11.84TFQPTVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.46NGRR28 pKa = 11.84KK29 pKa = 7.78TINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2981

0

2981

974289

35

4532

326.8

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.258 ± 0.049

1.049 ± 0.016

5.591 ± 0.054

6.007 ± 0.048

4.207 ± 0.035

6.824 ± 0.045

2.309 ± 0.024

6.737 ± 0.039

5.517 ± 0.04

10.228 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.025

4.333 ± 0.033

3.965 ± 0.025

4.738 ± 0.041

4.173 ± 0.039

6.636 ± 0.038

5.456 ± 0.048

6.93 ± 0.037

1.279 ± 0.018

3.032 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski