Mycobacterium phage Krili

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Corndogvirus; unclassified Corndogvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8DDL6|A0A5P8DDL6_9CAUD Uncharacterized protein OS=Mycobacterium phage Krili OX=2652893 GN=41 PE=4 SV=1
MM1 pKa = 7.26KK2 pKa = 10.16VWFQGRR8 pKa = 11.84YY9 pKa = 8.0IDD11 pKa = 3.66VAVTVVEE18 pKa = 4.4GDD20 pKa = 3.5DD21 pKa = 3.77GVPYY25 pKa = 10.57VYY27 pKa = 10.93VDD29 pKa = 3.77VPDD32 pKa = 4.8GPDD35 pKa = 3.19GGGEE39 pKa = 4.14PLPAAAA45 pKa = 5.69

Molecular weight: 4.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8DE77|A0A5P8DE77_9CAUD Exonuclease OS=Mycobacterium phage Krili OX=2652893 GN=13 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.66ADD5 pKa = 3.87TARR8 pKa = 11.84KK9 pKa = 9.87NGWKK13 pKa = 10.25VGTRR17 pKa = 11.84LAGDD21 pKa = 3.37EE22 pKa = 4.85GYY24 pKa = 10.96GEE26 pKa = 4.4TVIEE30 pKa = 3.89ITAIGEE36 pKa = 4.14AQVLAKK42 pKa = 9.93TITHH46 pKa = 7.49AGRR49 pKa = 11.84PAPYY53 pKa = 10.27GEE55 pKa = 4.42SLWTFVCRR63 pKa = 11.84DD64 pKa = 2.81WRR66 pKa = 11.84EE67 pKa = 3.77VPRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84CRR74 pKa = 11.84INDD77 pKa = 3.53RR78 pKa = 11.84LVRR81 pKa = 11.84TLRR84 pKa = 11.84RR85 pKa = 11.84IVLRR89 pKa = 11.84NGVPP93 pKa = 3.09

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

22853

30

1605

177.2

19.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.581 ± 0.324

1.238 ± 0.17

6.231 ± 0.202

6.704 ± 0.283

2.892 ± 0.141

8.41 ± 0.361

1.833 ± 0.12

4.49 ± 0.18

3.606 ± 0.196

7.938 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.125

3.102 ± 0.196

5.982 ± 0.202

3.422 ± 0.2

7.067 ± 0.269

5.487 ± 0.208

6.192 ± 0.175

7.557 ± 0.283

2.21 ± 0.158

2.481 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski