Maize fine streak nucleorhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6E0X8|Q6E0X8_9RHAB N protein OS=Maize fine streak nucleorhabdovirus OX=209854 GN=N PE=4 SV=1
MM1 pKa = 7.08SQRR4 pKa = 11.84TLRR7 pKa = 11.84STRR10 pKa = 11.84SSQSKK15 pKa = 10.3KK16 pKa = 10.43GFANLEE22 pKa = 4.18SYY24 pKa = 10.32RR25 pKa = 11.84EE26 pKa = 3.99KK27 pKa = 10.88SVTEE31 pKa = 3.75ADD33 pKa = 3.39PAYY36 pKa = 10.8SSVQIPHH43 pKa = 6.38SVTEE47 pKa = 4.0STRR50 pKa = 11.84YY51 pKa = 9.9KK52 pKa = 10.73SLEE55 pKa = 4.07QVDD58 pKa = 4.46KK59 pKa = 11.34LPLSSDD65 pKa = 2.52IPAQIEE71 pKa = 4.63SQVEE75 pKa = 3.84MAPILTPEE83 pKa = 4.23RR84 pKa = 11.84KK85 pKa = 8.24EE86 pKa = 4.16TMKK89 pKa = 11.17EE90 pKa = 3.95EE91 pKa = 3.71ITKK94 pKa = 10.25FLMARR99 pKa = 11.84GCVPDD104 pKa = 3.93NMAMEE109 pKa = 4.54QLLKK113 pKa = 10.64AHH115 pKa = 6.58EE116 pKa = 4.69PIIDD120 pKa = 3.79NPAADD125 pKa = 5.1IIPDD129 pKa = 3.77TTYY132 pKa = 11.44LSAFCQGWTFCMRR145 pKa = 11.84VYY147 pKa = 11.41SDD149 pKa = 3.28MALNNLKK156 pKa = 8.87KK157 pKa = 9.11TIEE160 pKa = 4.86PIMSTLAATTNTMQGLTGEE179 pKa = 4.66LNHH182 pKa = 6.46TSKK185 pKa = 10.99LLKK188 pKa = 10.32LRR190 pKa = 11.84VSAVPFKK197 pKa = 10.59RR198 pKa = 11.84QSPKK202 pKa = 10.86DD203 pKa = 3.27VAARR207 pKa = 11.84INKK210 pKa = 7.43MKK212 pKa = 10.57QKK214 pKa = 10.12GRR216 pKa = 11.84RR217 pKa = 11.84AEE219 pKa = 5.14DD220 pKa = 2.78IMDD223 pKa = 4.14PMKK226 pKa = 10.79QPMVEE231 pKa = 4.01EE232 pKa = 4.54EE233 pKa = 3.9EE234 pKa = 5.72DD235 pKa = 3.3IDD237 pKa = 4.69EE238 pKa = 4.48EE239 pKa = 4.87SEE241 pKa = 4.65DD242 pKa = 3.78VFPEE246 pKa = 4.14YY247 pKa = 10.57SSSLLRR253 pKa = 11.84TTWDD257 pKa = 3.39EE258 pKa = 3.87MSDD261 pKa = 3.59DD262 pKa = 4.3LKK264 pKa = 11.21RR265 pKa = 11.84SLLYY269 pKa = 10.78GIIKK273 pKa = 10.18AAMDD277 pKa = 3.52IEE279 pKa = 4.77DD280 pKa = 3.98VGEE283 pKa = 4.28LDD285 pKa = 3.92SDD287 pKa = 3.85TMNTIEE293 pKa = 4.82RR294 pKa = 11.84SLKK297 pKa = 10.29GSQLTTAYY305 pKa = 10.73LMKK308 pKa = 10.73DD309 pKa = 3.11EE310 pKa = 5.1GVDD313 pKa = 3.07RR314 pKa = 11.84DD315 pKa = 3.68KK316 pKa = 10.94IRR318 pKa = 11.84RR319 pKa = 11.84YY320 pKa = 9.22IKK322 pKa = 10.16KK323 pKa = 8.87IWEE326 pKa = 3.95QAGLPNKK333 pKa = 9.88MEE335 pKa = 4.73IGDD338 pKa = 3.74

Molecular weight: 38.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6E0X8|Q6E0X8_9RHAB N protein OS=Maize fine streak nucleorhabdovirus OX=209854 GN=N PE=4 SV=1
MM1 pKa = 7.66NYY3 pKa = 9.79NRR5 pKa = 11.84LKK7 pKa = 11.04FEE9 pKa = 4.77DD10 pKa = 4.07AFLTPSADD18 pKa = 3.19YY19 pKa = 10.99AAFKK23 pKa = 10.51TPLPYY28 pKa = 9.76PDD30 pKa = 4.54LTTGPTEE37 pKa = 3.89VPYY40 pKa = 10.52TKK42 pKa = 10.33EE43 pKa = 3.78KK44 pKa = 10.71LITASYY50 pKa = 8.98PYY52 pKa = 8.9WTMTKK57 pKa = 10.2VNSGDD62 pKa = 3.06IKK64 pKa = 10.17TLGKK68 pKa = 8.51TVLTKK73 pKa = 11.04LSDD76 pKa = 3.39KK77 pKa = 10.92KK78 pKa = 10.02ITEE81 pKa = 3.79KK82 pKa = 10.44TLFDD86 pKa = 3.66MCRR89 pKa = 11.84IALSLKK95 pKa = 10.21APNGQDD101 pKa = 2.99VFKK104 pKa = 10.83IPTVTFAGAKK114 pKa = 9.34TGGQDD119 pKa = 3.44DD120 pKa = 4.55TNLVPVPSDD129 pKa = 3.16TSKK132 pKa = 7.65TTEE135 pKa = 3.84YY136 pKa = 10.48LQTVTSKK143 pKa = 11.03SSTSQATQKK152 pKa = 10.78KK153 pKa = 9.49SLSTYY158 pKa = 10.01VDD160 pKa = 2.84NDD162 pKa = 3.41ANYY165 pKa = 7.27EE166 pKa = 4.02TAIPYY171 pKa = 7.35FLCSFLRR178 pKa = 11.84LIVKK182 pKa = 9.91QPEE185 pKa = 3.62SWRR188 pKa = 11.84RR189 pKa = 11.84AFGDD193 pKa = 3.13IKK195 pKa = 10.2EE196 pKa = 4.37QYY198 pKa = 10.2GKK200 pKa = 10.83FYY202 pKa = 11.1GKK204 pKa = 8.98TSNLITNAEE213 pKa = 4.34DD214 pKa = 3.87DD215 pKa = 4.18LSIASVVKK223 pKa = 10.52VAFDD227 pKa = 3.4TFKK230 pKa = 10.89PIVNFIAYY238 pKa = 9.19VAGEE242 pKa = 4.2TDD244 pKa = 4.19KK245 pKa = 11.55GLSPNTKK252 pKa = 7.47EE253 pKa = 4.59HH254 pKa = 6.57GMFTYY259 pKa = 10.16FVGQHH264 pKa = 5.71LSFVGMHH271 pKa = 6.5VYY273 pKa = 10.67PMVAEE278 pKa = 4.84LMQKK282 pKa = 10.37CKK284 pKa = 10.62GIKK287 pKa = 9.23QDD289 pKa = 4.26LFLTFLDD296 pKa = 3.33VDD298 pKa = 3.84EE299 pKa = 4.97TKK301 pKa = 10.91EE302 pKa = 4.2SVKK305 pKa = 10.2EE306 pKa = 3.52ICRR309 pKa = 11.84IMTTYY314 pKa = 10.61DD315 pKa = 3.24APSQTDD321 pKa = 3.12RR322 pKa = 11.84ASRR325 pKa = 11.84DD326 pKa = 3.71FLWKK330 pKa = 8.15YY331 pKa = 10.84ARR333 pKa = 11.84VIDD336 pKa = 3.78EE337 pKa = 4.53GFFLKK342 pKa = 10.67LQNKK346 pKa = 8.46KK347 pKa = 10.4NKK349 pKa = 9.34EE350 pKa = 3.91FLYY353 pKa = 10.85GLALIHH359 pKa = 6.51EE360 pKa = 4.65KK361 pKa = 9.91MGLVRR366 pKa = 11.84SVSYY370 pKa = 11.05AKK372 pKa = 8.14PTNMAILQNQDD383 pKa = 2.72GLRR386 pKa = 11.84ADD388 pKa = 3.58AEE390 pKa = 4.44EE391 pKa = 4.12YY392 pKa = 11.32ANLFVFLYY400 pKa = 10.55KK401 pKa = 10.49KK402 pKa = 10.12ATGQKK407 pKa = 9.62EE408 pKa = 4.3GASPMDD414 pKa = 3.42QLRR417 pKa = 11.84SQLAGEE423 pKa = 4.44RR424 pKa = 11.84VTATIAEE431 pKa = 4.08ASAPKK436 pKa = 10.06RR437 pKa = 11.84SSDD440 pKa = 3.16GTGNVSKK447 pKa = 10.75KK448 pKa = 10.14KK449 pKa = 10.08SRR451 pKa = 11.84KK452 pKa = 9.24DD453 pKa = 3.24DD454 pKa = 3.33TGIASMEE461 pKa = 4.07FF462 pKa = 3.31

Molecular weight: 51.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4006

93

1944

572.3

65.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.818 ± 0.583

1.523 ± 0.285

5.891 ± 0.442

6.166 ± 0.42

3.819 ± 0.458

5.117 ± 0.259

2.122 ± 0.279

7.639 ± 0.641

6.965 ± 0.574

9.411 ± 0.821

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.12 ± 0.316

4.269 ± 0.454

4.169 ± 0.171

3.27 ± 0.291

4.868 ± 0.32

8.462 ± 0.388

6.99 ± 0.827

5.542 ± 0.396

1.498 ± 0.234

4.343 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski