Influenza A virus (A/mallard/MN/51/1998(H2N3))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H2N3 subtype

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6EES3|B6EES3_9INFA RNA-directed RNA polymerase catalytic subunit OS=Influenza A virus (A/mallard/MN/51/1998(H2N3)) OX=566259 GN=PB1 PE=3 SV=1
MM1 pKa = 7.82EE2 pKa = 5.88DD3 pKa = 4.23FVRR6 pKa = 11.84QCFNPMIVEE15 pKa = 4.33LAEE18 pKa = 4.23KK19 pKa = 10.56AMKK22 pKa = 10.25EE23 pKa = 3.99YY24 pKa = 11.35GEE26 pKa = 4.4DD27 pKa = 3.65PKK29 pKa = 10.9IEE31 pKa = 4.27TNKK34 pKa = 9.66FAAICTHH41 pKa = 6.8LEE43 pKa = 3.9VCFMYY48 pKa = 10.77SDD50 pKa = 3.5FHH52 pKa = 8.77FIDD55 pKa = 3.6EE56 pKa = 4.51RR57 pKa = 11.84GEE59 pKa = 4.25SIIVEE64 pKa = 4.23SGDD67 pKa = 3.45PNALLKK73 pKa = 10.77HH74 pKa = 5.93RR75 pKa = 11.84FEE77 pKa = 6.44IIEE80 pKa = 4.15GRR82 pKa = 11.84DD83 pKa = 2.82RR84 pKa = 11.84TMAWTVVNSICNTTGVEE101 pKa = 4.2KK102 pKa = 10.67PKK104 pKa = 10.45FLPDD108 pKa = 3.39LYY110 pKa = 10.76DD111 pKa = 3.44YY112 pKa = 11.29KK113 pKa = 11.02EE114 pKa = 3.78NRR116 pKa = 11.84FIEE119 pKa = 4.17IGVTRR124 pKa = 11.84RR125 pKa = 11.84EE126 pKa = 3.6VHH128 pKa = 6.1IYY130 pKa = 10.32YY131 pKa = 10.35LEE133 pKa = 4.16KK134 pKa = 10.68ANKK137 pKa = 8.54IKK139 pKa = 10.7SEE141 pKa = 3.97KK142 pKa = 7.72THH144 pKa = 6.1IHH146 pKa = 5.76IFSFTGEE153 pKa = 3.88EE154 pKa = 4.15MATKK158 pKa = 10.42ADD160 pKa = 3.63YY161 pKa = 10.43TLDD164 pKa = 3.45EE165 pKa = 4.41EE166 pKa = 4.38SRR168 pKa = 11.84ARR170 pKa = 11.84IKK172 pKa = 10.03TRR174 pKa = 11.84LFTIRR179 pKa = 11.84QEE181 pKa = 4.07MASRR185 pKa = 11.84GLWDD189 pKa = 4.1PFRR192 pKa = 11.84QSEE195 pKa = 4.24RR196 pKa = 11.84GEE198 pKa = 3.89EE199 pKa = 4.16TIEE202 pKa = 3.68EE203 pKa = 4.17RR204 pKa = 11.84FEE206 pKa = 3.72ITGTMRR212 pKa = 11.84RR213 pKa = 11.84LADD216 pKa = 3.3QSLPPNFSSLEE227 pKa = 3.83NFRR230 pKa = 11.84AYY232 pKa = 11.05VDD234 pKa = 3.5GFEE237 pKa = 4.68PNGCIEE243 pKa = 4.47GKK245 pKa = 9.96LSQMSKK251 pKa = 9.86EE252 pKa = 3.84VNARR256 pKa = 11.84IEE258 pKa = 4.38PFLKK262 pKa = 8.15TTPRR266 pKa = 11.84PLRR269 pKa = 11.84LPEE272 pKa = 4.79GPPCSQRR279 pKa = 11.84SKK281 pKa = 10.91FLLMDD286 pKa = 4.4ALKK289 pKa = 10.88LSIEE293 pKa = 4.56DD294 pKa = 4.07PSHH297 pKa = 6.12EE298 pKa = 4.34GEE300 pKa = 5.39GIPLYY305 pKa = 10.72DD306 pKa = 5.54AIKK309 pKa = 10.45CMKK312 pKa = 9.82TFFGWKK318 pKa = 8.64EE319 pKa = 3.72PNIVKK324 pKa = 9.51PHH326 pKa = 6.17EE327 pKa = 4.45KK328 pKa = 10.38GINPNYY334 pKa = 9.91LLAWKK339 pKa = 9.87QVLAEE344 pKa = 4.25LQDD347 pKa = 3.52IEE349 pKa = 4.52NEE351 pKa = 4.07EE352 pKa = 4.77KK353 pKa = 10.44IPKK356 pKa = 7.57TKK358 pKa = 10.66NMKK361 pKa = 8.74KK362 pKa = 8.8TSQLKK367 pKa = 8.56WALGEE372 pKa = 3.97NMAPEE377 pKa = 4.21KK378 pKa = 10.99VDD380 pKa = 5.68FEE382 pKa = 4.64DD383 pKa = 4.93CKK385 pKa = 11.16DD386 pKa = 3.67VSDD389 pKa = 4.28LRR391 pKa = 11.84QYY393 pKa = 11.66DD394 pKa = 3.41SDD396 pKa = 3.88EE397 pKa = 4.32PEE399 pKa = 4.05QRR401 pKa = 11.84SLASWIQSEE410 pKa = 4.32FNKK413 pKa = 10.5ACEE416 pKa = 4.41LTDD419 pKa = 3.77SSWIEE424 pKa = 3.62LDD426 pKa = 4.1EE427 pKa = 5.66IGEE430 pKa = 4.15DD431 pKa = 3.56VAPIEE436 pKa = 5.34HH437 pKa = 6.74IASMRR442 pKa = 11.84RR443 pKa = 11.84NYY445 pKa = 8.17FTAEE449 pKa = 3.92VSHH452 pKa = 6.6CRR454 pKa = 11.84ATEE457 pKa = 3.87YY458 pKa = 10.63IMKK461 pKa = 10.18GVYY464 pKa = 10.04INTALLNASCAAMDD478 pKa = 4.67DD479 pKa = 4.09FQLIPMISKK488 pKa = 10.11CRR490 pKa = 11.84TKK492 pKa = 10.42EE493 pKa = 3.77GRR495 pKa = 11.84RR496 pKa = 11.84KK497 pKa = 7.6TNLYY501 pKa = 10.52GFIIKK506 pKa = 9.94GRR508 pKa = 11.84SHH510 pKa = 7.02LRR512 pKa = 11.84NDD514 pKa = 3.25TDD516 pKa = 3.55VVNFVSMEE524 pKa = 4.12FSLTDD529 pKa = 3.59PRR531 pKa = 11.84LEE533 pKa = 3.7PHH535 pKa = 6.34KK536 pKa = 10.03WEE538 pKa = 4.59KK539 pKa = 11.01YY540 pKa = 8.74CVLEE544 pKa = 5.19IGDD547 pKa = 4.0MLLRR551 pKa = 11.84TAIGQVSRR559 pKa = 11.84PMFLYY564 pKa = 10.52VRR566 pKa = 11.84TNGTSKK572 pKa = 10.61IKK574 pKa = 9.96MKK576 pKa = 9.82WGMEE580 pKa = 3.71MRR582 pKa = 11.84RR583 pKa = 11.84CLLQSLQQIEE593 pKa = 4.49SMIEE597 pKa = 3.69AEE599 pKa = 4.34SSVKK603 pKa = 10.34EE604 pKa = 3.72KK605 pKa = 11.43DD606 pKa = 3.06MTKK609 pKa = 10.59EE610 pKa = 3.6FFEE613 pKa = 4.76NKK615 pKa = 9.96SEE617 pKa = 3.88TWPIGEE623 pKa = 4.22SPKK626 pKa = 10.75GVEE629 pKa = 4.03EE630 pKa = 4.32GSIGKK635 pKa = 8.56VCRR638 pKa = 11.84TLLAKK643 pKa = 10.3SVFNSLYY650 pKa = 10.64ASPQLEE656 pKa = 4.23GFSAEE661 pKa = 4.07SRR663 pKa = 11.84KK664 pKa = 10.13LLLIVQALMDD674 pKa = 4.2NLEE677 pKa = 4.82PGTFDD682 pKa = 5.09LGGLYY687 pKa = 9.85EE688 pKa = 5.25AIEE691 pKa = 4.38EE692 pKa = 4.37CLINDD697 pKa = 3.63PWVLLNASWFNSFLTHH713 pKa = 6.92ALKK716 pKa = 10.78

Molecular weight: 82.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6EES5|B6EES5_9INFA Polymerase basic protein 2 OS=Influenza A virus (A/mallard/MN/51/1998(H2N3)) OX=566259 GN=PB2 PE=3 SV=1
MM1 pKa = 7.42EE2 pKa = 5.8RR3 pKa = 11.84EE4 pKa = 3.77QDD6 pKa = 4.01TPWTQSTEE14 pKa = 4.0HH15 pKa = 7.17INIQKK20 pKa = 10.18RR21 pKa = 11.84GNGQPTQRR29 pKa = 11.84LEE31 pKa = 4.29HH32 pKa = 6.81PNLTQLTDD40 pKa = 2.94HH41 pKa = 7.36CLRR44 pKa = 11.84TMSQVDD50 pKa = 3.29MHH52 pKa = 6.48KK53 pKa = 9.27QTVSLKK59 pKa = 9.82QWLSSKK65 pKa = 10.89NPTQEE70 pKa = 3.97SLKK73 pKa = 9.98TRR75 pKa = 11.84VLKK78 pKa = 10.36RR79 pKa = 11.84WKK81 pKa = 10.33LFNKK85 pKa = 9.41QEE87 pKa = 4.01WTNN90 pKa = 3.4

Molecular weight: 10.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4333

90

759

481.4

54.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.724 ± 0.526

1.777 ± 0.4

4.662 ± 0.289

7.385 ± 0.744

3.785 ± 0.288

6.67 ± 0.464

1.708 ± 0.184

6.531 ± 0.44

5.977 ± 0.464

7.824 ± 0.499

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.9 ± 0.48

5.562 ± 0.484

4.108 ± 0.296

3.877 ± 0.466

6.739 ± 0.794

6.993 ± 0.192

6.97 ± 0.442

5.608 ± 0.513

1.592 ± 0.19

2.608 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski