Chloroflexi bacterium (strain G233)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; unclassified Chloroflexi

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2626 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A9HCZ3|A0A2A9HCZ3_CHLB2 4-hydroxybutyrate CoA-transferase OS=Chloroflexi bacterium (strain G233) OX=2761530 GN=A9A59_0069 PE=3 SV=1
MM1 pKa = 7.97DD2 pKa = 5.62PNVIVNHH9 pKa = 4.98VMRR12 pKa = 11.84LVRR15 pKa = 11.84FDD17 pKa = 3.32TSVFDD22 pKa = 4.97EE23 pKa = 4.44VRR25 pKa = 11.84DD26 pKa = 3.89DD27 pKa = 4.2ANEE30 pKa = 4.83LIPALVVAAVASLLAGLGATLFWEE54 pKa = 5.09FNFDD58 pKa = 5.58FGPEE62 pKa = 4.09DD63 pKa = 3.1PWLNTFILGGIFMAALYY80 pKa = 9.62AVWVLVVYY88 pKa = 10.69VMLAQVYY95 pKa = 8.84KK96 pKa = 11.0ASGDD100 pKa = 3.69VQSLFRR106 pKa = 11.84TMGYY110 pKa = 10.16AAWPLALSVLMFIPVLYY127 pKa = 9.87PVFALGSIVLLFVMSIYY144 pKa = 10.55AAQSVTNADD153 pKa = 3.41STQVVMANLAGMAVFTLVLSIIAFSSDD180 pKa = 2.88TTIIGAGIFAILQQIPP196 pKa = 3.21

Molecular weight: 21.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A9HDV5|A0A2A9HDV5_CHLB2 Uncharacterized protein OS=Chloroflexi bacterium (strain G233) OX=2761530 GN=A9A59_0172 PE=4 SV=1
MM1 pKa = 7.29TRR3 pKa = 11.84PTSPLPRR10 pKa = 11.84RR11 pKa = 11.84AAVVLAQGAGAALIAAATGPLGGLVAAAALGLPPVLAAALARR53 pKa = 11.84RR54 pKa = 11.84LAA56 pKa = 3.88

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2626

0

2626

831118

37

2436

316.5

34.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.545 ± 0.081

0.846 ± 0.015

5.142 ± 0.034

6.651 ± 0.056

3.405 ± 0.026

9.002 ± 0.046

1.874 ± 0.019

4.551 ± 0.044

2.069 ± 0.037

10.163 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.955 ± 0.019

2.104 ± 0.028

6.239 ± 0.05

2.862 ± 0.024

8.482 ± 0.054

4.267 ± 0.031

5.088 ± 0.047

7.995 ± 0.034

1.474 ± 0.022

2.285 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski