Nectarine stem pitting associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4HNN4|A0A0U4HNN4_9LUTE RNA dependent RNA polymerase P1 OS=Nectarine stem pitting associated virus OX=2560614 GN=NSPaV_SF04522E_gp2 PE=4 SV=1
MM1 pKa = 7.85IFDD4 pKa = 5.02LLISASTKK12 pKa = 10.14AIKK15 pKa = 10.55DD16 pKa = 4.22FISFLYY22 pKa = 10.07SKK24 pKa = 10.33CRR26 pKa = 11.84NIYY29 pKa = 10.41CRR31 pKa = 11.84FKK33 pKa = 10.41KK34 pKa = 9.71WLMDD38 pKa = 3.78FSEE41 pKa = 4.39YY42 pKa = 10.88DD43 pKa = 3.27AFVAEE48 pKa = 4.83CFEE51 pKa = 4.48TMFEE55 pKa = 4.23VEE57 pKa = 4.38TFQEE61 pKa = 4.29EE62 pKa = 4.6VVDD65 pKa = 3.8AFIRR69 pKa = 11.84IEE71 pKa = 4.39DD72 pKa = 3.65EE73 pKa = 3.86LAAAEE78 pKa = 4.25AKK80 pKa = 10.39LKK82 pKa = 10.1EE83 pKa = 4.2VHH85 pKa = 6.64NPYY88 pKa = 10.57LWGKK92 pKa = 7.6ISEE95 pKa = 4.71YY96 pKa = 10.11IFPSRR101 pKa = 11.84PEE103 pKa = 3.88DD104 pKa = 3.48VEE106 pKa = 4.33VAKK109 pKa = 10.8LQVYY113 pKa = 9.96AKK115 pKa = 8.59EE116 pKa = 3.8VQMRR120 pKa = 11.84VFIDD124 pKa = 3.48DD125 pKa = 3.91TLDD128 pKa = 3.7DD129 pKa = 3.88MEE131 pKa = 4.6EE132 pKa = 4.18AVSGTMSEE140 pKa = 4.33SQVEE144 pKa = 4.49VLALTPNQLKK154 pKa = 10.68ARR156 pKa = 11.84LKK158 pKa = 10.16KK159 pKa = 9.52AAKK162 pKa = 9.59HH163 pKa = 5.42RR164 pKa = 11.84RR165 pKa = 11.84QKK167 pKa = 10.15QAAKK171 pKa = 10.37RR172 pKa = 11.84MRR174 pKa = 11.84EE175 pKa = 3.92AMDD178 pKa = 3.39KK179 pKa = 10.56VEE181 pKa = 5.64KK182 pKa = 10.2IAEE185 pKa = 4.23LSDD188 pKa = 2.94WTTFQHH194 pKa = 6.7VEE196 pKa = 4.13VVDD199 pKa = 4.19QKK201 pKa = 10.79HH202 pKa = 4.74SHH204 pKa = 6.57PARR207 pKa = 11.84EE208 pKa = 4.16EE209 pKa = 3.66QGEE212 pKa = 4.22DD213 pKa = 3.01GKK215 pKa = 11.34KK216 pKa = 10.11IIPAKK221 pKa = 9.87IAKK224 pKa = 8.9KK225 pKa = 9.25WIWVRR230 pKa = 11.84NIKK233 pKa = 9.42TGEE236 pKa = 4.04EE237 pKa = 3.25KK238 pKa = 10.42RR239 pKa = 11.84ARR241 pKa = 11.84HH242 pKa = 6.29FIRR245 pKa = 11.84AYY247 pKa = 10.84VMSKK251 pKa = 9.69NLRR254 pKa = 11.84LRR256 pKa = 11.84GDD258 pKa = 3.81DD259 pKa = 3.52VSKK262 pKa = 10.17VTIQRR267 pKa = 11.84YY268 pKa = 8.3VEE270 pKa = 4.11QFCDD274 pKa = 3.62ANDD277 pKa = 3.77FSLEE281 pKa = 4.19AKK283 pKa = 8.29TQLIKK288 pKa = 10.61VALMMVPVPTKK299 pKa = 10.36TEE301 pKa = 3.16IDD303 pKa = 3.38MAMVVHH309 pKa = 6.77CPRR312 pKa = 11.84AEE314 pKa = 3.99ALRR317 pKa = 11.84HH318 pKa = 4.52QLEE321 pKa = 4.91CIEE324 pKa = 4.34SQVFF328 pKa = 3.22

Molecular weight: 38.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4H028|A0A0U4H028_9LUTE Readthrough protein OS=Nectarine stem pitting associated virus OX=2560614 GN=NSPaV_SF04522E_gp3 PE=3 SV=1
MM1 pKa = 7.02SQRR4 pKa = 11.84NRR6 pKa = 11.84RR7 pKa = 11.84SQNAGWLPPMRR18 pKa = 11.84QQPPRR23 pKa = 11.84WSSQRR28 pKa = 11.84QGAPAPRR35 pKa = 11.84IVYY38 pKa = 7.96VTGPPVQPKK47 pKa = 9.66KK48 pKa = 10.12AAKK51 pKa = 9.79KK52 pKa = 9.8KK53 pKa = 10.4SPPSKK58 pKa = 10.18QPQPTSPFPAFKK70 pKa = 9.65FTIDD74 pKa = 4.22DD75 pKa = 4.15LKK77 pKa = 11.45GDD79 pKa = 3.51ASGVLKK85 pKa = 10.56FGPKK89 pKa = 9.42LDD91 pKa = 3.56QYY93 pKa = 10.94QAFVNGIMKK102 pKa = 10.28SFHH105 pKa = 7.69DD106 pKa = 3.99YY107 pKa = 10.46RR108 pKa = 11.84ISSVVIRR115 pKa = 11.84YY116 pKa = 8.41VSNAASTTPGAMAFEE131 pKa = 5.39IDD133 pKa = 3.77TSCTQTEE140 pKa = 4.29LSSKK144 pKa = 10.61VMSSPLNRR152 pKa = 11.84SFTKK156 pKa = 10.03TFSGPTIRR164 pKa = 11.84GNLWLNTKK172 pKa = 8.76QEE174 pKa = 4.3QFWLLYY180 pKa = 9.05KK181 pKa = 11.02ANGAKK186 pKa = 9.89SDD188 pKa = 3.32IAGQFIITLHH198 pKa = 5.23THH200 pKa = 4.96WQNPKK205 pKa = 9.99SS206 pKa = 3.69

Molecular weight: 23.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1907

206

847

476.8

54.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.289 ± 0.616

1.993 ± 0.25

5.349 ± 0.262

6.397 ± 1.14

5.034 ± 0.096

4.667 ± 0.654

2.307 ± 0.121

5.087 ± 0.445

7.708 ± 0.512

6.502 ± 0.914

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.458

4.038 ± 0.521

5.611 ± 1.406

4.51 ± 0.592

5.663 ± 0.504

7.656 ± 0.95

4.772 ± 0.68

7.551 ± 0.456

1.783 ± 0.396

3.146 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski