Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) (Poplar leaf rust fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Pucciniomycetes; Pucciniales; Melampsoraceae; Melampsora; Melampsora larici-populina

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4RRX9|F4RRX9_MELLP Uncharacterized protein OS=Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) OX=747676 GN=MELLADRAFT_72285 PE=4 SV=1
MM1 pKa = 8.0PNGQKK6 pKa = 10.15QLIPNAGQVAFLHH19 pKa = 6.63DD20 pKa = 3.85VWEE23 pKa = 4.63CLLPTQHH30 pKa = 6.69PNEE33 pKa = 4.41RR34 pKa = 11.84LPDD37 pKa = 3.49AEE39 pKa = 4.32FTAKK43 pKa = 10.18YY44 pKa = 9.71LKK46 pKa = 10.49SLSEE50 pKa = 4.27PYY52 pKa = 10.29RR53 pKa = 11.84GTLYY57 pKa = 11.05DD58 pKa = 3.99LDD60 pKa = 4.24EE61 pKa = 4.96DD62 pKa = 5.48ADD64 pKa = 3.99YY65 pKa = 11.61KK66 pKa = 11.38DD67 pKa = 5.46DD68 pKa = 3.94NDD70 pKa = 4.06EE71 pKa = 4.14EE72 pKa = 4.73RR73 pKa = 11.84GSNGMCILLSATNMPTSDD91 pKa = 4.17EE92 pKa = 4.45SKK94 pKa = 10.21CYY96 pKa = 10.26CDD98 pKa = 4.31GDD100 pKa = 3.83VGSLYY105 pKa = 10.8NSNDD109 pKa = 3.11EE110 pKa = 5.07DD111 pKa = 4.41VSQGPADD118 pKa = 4.51DD119 pKa = 5.32ADD121 pKa = 3.57MDD123 pKa = 4.0AA124 pKa = 5.5

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4S4N4|F4S4N4_MELLP Uncharacterized protein OS=Melampsora larici-populina (strain 98AG31 / pathotype 3-4-7) OX=747676 GN=MELLADRAFT_93289 PE=4 SV=1
MM1 pKa = 8.07ILINTKK7 pKa = 9.64NQTPSTIMIHH17 pKa = 6.87PSTTTPSPTPKK28 pKa = 10.31TMILINMKK36 pKa = 10.01NQIPRR41 pKa = 11.84KK42 pKa = 9.76IIIHH46 pKa = 6.88LNTTTPSPRR55 pKa = 11.84PMTPTNTKK63 pKa = 9.16NQTPRR68 pKa = 11.84TIMIHH73 pKa = 6.71PSTTTPSPRR82 pKa = 11.84TTILTNTKK90 pKa = 9.13NQTPIMIHH98 pKa = 6.8LNTTTPSPRR107 pKa = 11.84TIMILISIQNQTPRR121 pKa = 11.84KK122 pKa = 9.83IMIHH126 pKa = 6.71LNTTTPSPPPRR137 pKa = 11.84TIMIHH142 pKa = 6.76LNTTTSSPRR151 pKa = 11.84PMIPTSMKK159 pKa = 10.01NQTPRR164 pKa = 11.84NIMIHH169 pKa = 6.81LNTTTPSPRR178 pKa = 11.84PMIPTSIKK186 pKa = 10.15NQTPRR191 pKa = 11.84NIMIHH196 pKa = 6.81LNTTTPSQSPRR207 pKa = 11.84TIMTLISIKK216 pKa = 10.57NQTPRR221 pKa = 11.84KK222 pKa = 7.89ITIHH226 pKa = 6.95PSTTTPTPTPRR237 pKa = 11.84TIMILINMTNLIPRR251 pKa = 11.84KK252 pKa = 9.81IMIHH256 pKa = 7.22INMKK260 pKa = 10.01NQTEE264 pKa = 4.09

Molecular weight: 29.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16237

0

16237

6087715

34

5272

374.9

41.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.488 ± 0.019

1.4 ± 0.007

5.504 ± 0.012

6.055 ± 0.019

3.694 ± 0.013

5.712 ± 0.021

2.796 ± 0.012

5.582 ± 0.015

5.86 ± 0.021

9.192 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.008

4.722 ± 0.015

6.084 ± 0.022

4.444 ± 0.015

5.393 ± 0.017

9.581 ± 0.028

6.194 ± 0.014

5.375 ± 0.015

1.273 ± 0.007

2.552 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski