Drosophila affinis sigmavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140D8K1|A0A140D8K1_9RHAB PP3 OS=Drosophila affinis sigmavirus OX=1308859 GN=X PE=4 SV=1
MM1 pKa = 7.56GKK3 pKa = 9.28KK4 pKa = 8.11QEE6 pKa = 4.12RR7 pKa = 11.84KK8 pKa = 9.41KK9 pKa = 11.13DD10 pKa = 3.67KK11 pKa = 11.17ADD13 pKa = 3.43TNYY16 pKa = 8.62DD17 pKa = 3.56TLVALTKK24 pKa = 10.34QAIADD29 pKa = 3.85NPGLGDD35 pKa = 3.77NLQGDD40 pKa = 4.67VNLQPASLEE49 pKa = 4.01IPGMGDD55 pKa = 3.0KK56 pKa = 11.13SEE58 pKa = 4.91LPSDD62 pKa = 4.5DD63 pKa = 4.15DD64 pKa = 5.1RR65 pKa = 11.84SDD67 pKa = 3.31GGEE70 pKa = 3.99DD71 pKa = 3.47LGDD74 pKa = 4.35IIASGGASTSQGARR88 pKa = 11.84NIIDD92 pKa = 4.07PDD94 pKa = 3.61SDD96 pKa = 3.95GEE98 pKa = 4.17SDD100 pKa = 3.89YY101 pKa = 11.77KK102 pKa = 11.09DD103 pKa = 3.75GEE105 pKa = 4.45VPLPINPKK113 pKa = 9.57RR114 pKa = 11.84VTVFYY119 pKa = 10.65RR120 pKa = 11.84PPAYY124 pKa = 9.94QHH126 pKa = 6.32AEE128 pKa = 3.81KK129 pKa = 10.15IIRR132 pKa = 11.84DD133 pKa = 3.64TVEE136 pKa = 4.0AVNKK140 pKa = 9.86LLTYY144 pKa = 10.59EE145 pKa = 4.25SVTTEE150 pKa = 3.74GHH152 pKa = 6.05GEE154 pKa = 4.03SFMLCVYY161 pKa = 10.5PRR163 pKa = 11.84MTNPVTPILEE173 pKa = 4.64NILPGHH179 pKa = 6.45RR180 pKa = 11.84EE181 pKa = 3.87HH182 pKa = 6.05VQKK185 pKa = 10.85AIEE188 pKa = 4.39STDD191 pKa = 3.4QPKK194 pKa = 10.23PCIVDD199 pKa = 3.46QPSIPIDD206 pKa = 3.37RR207 pKa = 11.84RR208 pKa = 11.84SALLEE213 pKa = 3.65KK214 pKa = 10.19NYY216 pKa = 10.11CVKK219 pKa = 10.77YY220 pKa = 10.58LDD222 pKa = 3.56QKK224 pKa = 10.31NPKK227 pKa = 9.06VYY229 pKa = 10.51KK230 pKa = 9.86IDD232 pKa = 3.52NGNPDD237 pKa = 4.07FSPEE241 pKa = 3.79KK242 pKa = 10.35FQEE245 pKa = 5.16FYY247 pKa = 10.95CKK249 pKa = 10.61YY250 pKa = 10.41QGFPKK255 pKa = 10.24NDD257 pKa = 3.35YY258 pKa = 9.96NLVRR262 pKa = 11.84RR263 pKa = 11.84MLMEE267 pKa = 3.37QSKK270 pKa = 8.7WYY272 pKa = 10.09RR273 pKa = 11.84YY274 pKa = 10.37NDD276 pKa = 3.55VLDD279 pKa = 4.38FAPLRR284 pKa = 4.14

Molecular weight: 32.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140D8K2|A0A140D8K2_9RHAB Matrix protein OS=Drosophila affinis sigmavirus OX=1308859 GN=M PE=4 SV=1
MM1 pKa = 7.56LIIWIAQGSICSSFEE16 pKa = 4.42LICRR20 pKa = 11.84CDD22 pKa = 3.75LQNTTSLNPDD32 pKa = 2.89HH33 pKa = 6.91VARR36 pKa = 11.84AVTTYY41 pKa = 9.9YY42 pKa = 10.17IPNLDD47 pKa = 4.16RR48 pKa = 11.84YY49 pKa = 10.95NITQEE54 pKa = 3.94MKK56 pKa = 9.99IKK58 pKa = 10.28VMKK61 pKa = 9.92GGQVIDD67 pKa = 4.36TYY69 pKa = 11.61NQICLHH75 pKa = 6.42MGPMAGVTTHH85 pKa = 6.52IPVSEE90 pKa = 4.67LNSQSAAILRR100 pKa = 11.84QINSKK105 pKa = 10.61RR106 pKa = 11.84NSTVLDD112 pKa = 3.8GSVPSDD118 pKa = 3.2RR119 pKa = 11.84CRR121 pKa = 11.84RR122 pKa = 11.84GYY124 pKa = 10.78CSISTTEE131 pKa = 3.67RR132 pKa = 11.84SKK134 pKa = 11.13DD135 pKa = 3.47GKK137 pKa = 9.94PPEE140 pKa = 4.39TPVYY144 pKa = 10.16TIVTSNISIEE154 pKa = 4.02TDD156 pKa = 3.06TFNTVGKK163 pKa = 9.81EE164 pKa = 3.67VDD166 pKa = 4.72DD167 pKa = 4.74IDD169 pKa = 3.83TASYY173 pKa = 11.12LNDD176 pKa = 3.5IEE178 pKa = 5.29PMFPHH183 pKa = 6.61HH184 pKa = 7.34RR185 pKa = 11.84ILHH188 pKa = 5.85KK189 pKa = 10.69DD190 pKa = 3.27YY191 pKa = 10.97LIRR194 pKa = 11.84GLQQEE199 pKa = 4.47QEE201 pKa = 4.22TEE203 pKa = 3.72LAYY206 pKa = 10.97SKK208 pKa = 11.16NDD210 pKa = 2.99YY211 pKa = 10.36NRR213 pKa = 11.84RR214 pKa = 11.84KK215 pKa = 9.46RR216 pKa = 11.84HH217 pKa = 5.89ASPAHH222 pKa = 5.42HH223 pKa = 5.81QRR225 pKa = 11.84HH226 pKa = 5.87RR227 pKa = 11.84IINRR231 pKa = 11.84QLGMLINRR239 pKa = 11.84LNLYY243 pKa = 9.77EE244 pKa = 4.48SLWIQNYY251 pKa = 9.98IIMLVYY257 pKa = 10.3IKK259 pKa = 9.86KK260 pKa = 8.59TEE262 pKa = 4.07NPDD265 pKa = 3.06FTHH268 pKa = 6.16CVEE271 pKa = 4.25YY272 pKa = 10.09PSLRR276 pKa = 11.84DD277 pKa = 3.41MLRR280 pKa = 11.84CYY282 pKa = 11.06ADD284 pKa = 3.45NLEE287 pKa = 4.12TLYY290 pKa = 11.32NSDD293 pKa = 3.65YY294 pKa = 11.47ALDD297 pKa = 3.37AMRR300 pKa = 11.84YY301 pKa = 5.74FTEE304 pKa = 4.57GTQVKK309 pKa = 9.28YY310 pKa = 10.67NYY312 pKa = 9.8RR313 pKa = 11.84VEE315 pKa = 4.23EE316 pKa = 3.83QVKK319 pKa = 9.96YY320 pKa = 10.44KK321 pKa = 10.87LEE323 pKa = 4.04NRR325 pKa = 11.84LHH327 pKa = 5.61QKK329 pKa = 9.32RR330 pKa = 11.84HH331 pKa = 4.71NLRR334 pKa = 11.84GYY336 pKa = 10.39

Molecular weight: 39.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4081

226

2141

680.2

77.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.656 ± 0.636

1.862 ± 0.262

5.954 ± 0.654

5.146 ± 0.322

4.509 ± 0.584

5.66 ± 0.446

2.94 ± 0.351

8.037 ± 0.382

5.881 ± 0.374

8.895 ± 0.542

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.167

5.023 ± 0.416

5.415 ± 0.651

3.48 ± 0.379

5.072 ± 0.501

7.964 ± 0.555

6.175 ± 0.385

5.391 ± 0.269

1.642 ± 0.265

3.994 ± 0.525
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski