Mucilaginibacter pineti

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7C6S1|A0A1G7C6S1_9SPHI Metal-dependent hydrolase beta-lactamase superfamily II OS=Mucilaginibacter pineti OX=1391627 GN=SAMN05216464_105306 PE=4 SV=1
MM1 pKa = 8.35VEE3 pKa = 3.92VFKK6 pKa = 11.12TNVQDD11 pKa = 3.56MDD13 pKa = 3.58EE14 pKa = 5.07SEE16 pKa = 4.16VLIYY20 pKa = 10.2IIQKK24 pKa = 8.43QVPNSCITFDD34 pKa = 6.38LDD36 pKa = 3.98DD37 pKa = 5.09CDD39 pKa = 4.27NILRR43 pKa = 11.84VEE45 pKa = 4.58GEE47 pKa = 4.56DD48 pKa = 3.53ISPKK52 pKa = 10.49CVIDD56 pKa = 3.78LLNTYY61 pKa = 10.45GYY63 pKa = 10.01LCEE66 pKa = 4.14MLL68 pKa = 5.03

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6Y0D6|A0A1G6Y0D6_9SPHI HD domain-containing protein OS=Mucilaginibacter pineti OX=1391627 GN=SAMN05216464_102709 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 5.03GGGEE6 pKa = 4.13LGFLGWICKK15 pKa = 8.95PEE17 pKa = 4.05KK18 pKa = 9.52HH19 pKa = 5.99TPPPHH24 pKa = 5.74AVARR28 pKa = 11.84LLPTGVGKK36 pKa = 8.63TEE38 pKa = 4.53FYY40 pKa = 10.51KK41 pKa = 10.83KK42 pKa = 9.89IVISIEE48 pKa = 3.55PRR50 pKa = 11.84RR51 pKa = 11.84EE52 pKa = 3.24KK53 pKa = 10.7ARR55 pKa = 11.84RR56 pKa = 11.84EE57 pKa = 3.78RR58 pKa = 11.84EE59 pKa = 4.01IFCGIQSSNAWRR71 pKa = 11.84IRR73 pKa = 11.84FLARR77 pKa = 11.84TSHH80 pKa = 6.51RR81 pKa = 11.84NDD83 pKa = 3.49NIFVFPTPVGSCRR96 pKa = 11.84VTTINRR102 pKa = 11.84PPACEE107 pKa = 3.57LHH109 pKa = 5.84NQNNHH114 pKa = 5.91GGGTPRR120 pKa = 11.84NNRR123 pKa = 11.84HH124 pKa = 5.07SAGAEE129 pKa = 3.72RR130 pKa = 11.84LRR132 pKa = 4.63

Molecular weight: 14.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6283

0

6283

2125137

29

2988

338.2

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.701 ± 0.031

0.793 ± 0.009

5.479 ± 0.023

5.188 ± 0.036

4.881 ± 0.021

6.802 ± 0.032

1.913 ± 0.013

7.235 ± 0.028

6.983 ± 0.027

9.57 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.013

5.695 ± 0.031

3.909 ± 0.016

3.863 ± 0.019

3.851 ± 0.022

6.196 ± 0.026

5.988 ± 0.032

6.287 ± 0.024

1.206 ± 0.012

4.242 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski