Lake Sarah-associated circular virus-2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQJ4|A0A140AQJ4_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-2 OX=1685746 PE=3 SV=1
MM1 pKa = 7.35QINAKK6 pKa = 10.16SFFLTYY12 pKa = 9.29PRR14 pKa = 11.84CAITAEE20 pKa = 4.34TFQLWLEE27 pKa = 4.37STFTPTYY34 pKa = 10.47LVIGSEE40 pKa = 4.15LHH42 pKa = 6.27QDD44 pKa = 3.83GTPHH48 pKa = 5.28VHH50 pKa = 6.07VCFTLDD56 pKa = 2.94TALRR60 pKa = 11.84TRR62 pKa = 11.84DD63 pKa = 3.26EE64 pKa = 4.34RR65 pKa = 11.84VFDD68 pKa = 3.97FHH70 pKa = 6.8GHH72 pKa = 6.01HH73 pKa = 7.05PNIVRR78 pKa = 11.84PRR80 pKa = 11.84AIKK83 pKa = 10.35KK84 pKa = 10.36CIDD87 pKa = 3.48YY88 pKa = 10.84VKK90 pKa = 10.73KK91 pKa = 10.57DD92 pKa = 3.44GNFIEE97 pKa = 5.19SGEE100 pKa = 4.04PPAMKK105 pKa = 10.22RR106 pKa = 11.84KK107 pKa = 8.94WSEE110 pKa = 3.68LQTATTSEE118 pKa = 4.38EE119 pKa = 4.07FFKK122 pKa = 10.57TALEE126 pKa = 3.93ISPRR130 pKa = 11.84DD131 pKa = 3.38YY132 pKa = 11.48YY133 pKa = 11.48LNQEE137 pKa = 4.12RR138 pKa = 11.84LEE140 pKa = 4.07YY141 pKa = 9.45MARR144 pKa = 11.84KK145 pKa = 9.18LFKK148 pKa = 10.12PVVPTYY154 pKa = 10.32VPQWVDD160 pKa = 3.75FNIPQEE166 pKa = 4.05LSDD169 pKa = 4.65WIDD172 pKa = 3.29QRR174 pKa = 11.84HH175 pKa = 4.61EE176 pKa = 3.75VCYY179 pKa = 10.33RR180 pKa = 11.84PQSLILYY187 pKa = 7.07GASRR191 pKa = 11.84TGKK194 pKa = 7.82TEE196 pKa = 3.71WARR199 pKa = 11.84SLGPHH204 pKa = 6.33VYY206 pKa = 10.2FNGYY210 pKa = 10.11FMLDD214 pKa = 4.12LIRR217 pKa = 11.84DD218 pKa = 3.89DD219 pKa = 3.52VEE221 pKa = 3.94YY222 pKa = 10.83AIFDD226 pKa = 4.23DD227 pKa = 4.94FEE229 pKa = 5.96DD230 pKa = 3.34WTTFKK235 pKa = 10.67QYY237 pKa = 10.67KK238 pKa = 8.18QWLGAQKK245 pKa = 10.53QFVCTDD251 pKa = 3.51KK252 pKa = 11.09YY253 pKa = 10.63RR254 pKa = 11.84KK255 pKa = 9.78KK256 pKa = 10.94VDD258 pKa = 4.08LLWGKK263 pKa = 9.36PCIILSNEE271 pKa = 3.55YY272 pKa = 9.83PAFRR276 pKa = 11.84DD277 pKa = 3.54QDD279 pKa = 4.08WILLNCITVKK289 pKa = 10.58LNKK292 pKa = 9.39PLYY295 pKa = 9.95

Molecular weight: 35.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQJ4|A0A140AQJ4_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-2 OX=1685746 PE=3 SV=1
MM1 pKa = 7.3PRR3 pKa = 11.84SVSRR7 pKa = 11.84GRR9 pKa = 11.84SVSRR13 pKa = 11.84GSGAYY18 pKa = 6.43PTPSGKK24 pKa = 10.13RR25 pKa = 11.84GGPSGKK31 pKa = 9.35RR32 pKa = 11.84GRR34 pKa = 11.84STTRR38 pKa = 11.84SSKK41 pKa = 10.66GKK43 pKa = 9.42SKK45 pKa = 9.53RR46 pKa = 11.84TKK48 pKa = 9.46RR49 pKa = 11.84SHH51 pKa = 5.42SRR53 pKa = 11.84SRR55 pKa = 11.84SHH57 pKa = 7.15RR58 pKa = 11.84GKK60 pKa = 9.96HH61 pKa = 5.04VYY63 pKa = 9.09NPSMIAGGRR72 pKa = 11.84SQAVIKK78 pKa = 10.5LGKK81 pKa = 9.77DD82 pKa = 2.74KK83 pKa = 10.95KK84 pKa = 10.64LPKK87 pKa = 10.1FMKK90 pKa = 10.21FVSQPITHH98 pKa = 7.34IDD100 pKa = 3.12TGAVIVKK107 pKa = 10.04DD108 pKa = 3.99VAGKK112 pKa = 9.73AAYY115 pKa = 9.65SYY117 pKa = 10.88HH118 pKa = 6.54EE119 pKa = 4.43LMHH122 pKa = 5.52YY123 pKa = 10.18TDD125 pKa = 4.73MNRR128 pKa = 11.84VWLAAARR135 pKa = 11.84ANRR138 pKa = 11.84ATVVVGGADD147 pKa = 3.43VPKK150 pKa = 9.73WDD152 pKa = 4.66EE153 pKa = 4.11KK154 pKa = 10.27NHH156 pKa = 7.08SIFLQDD162 pKa = 2.47GHH164 pKa = 8.28INIRR168 pKa = 11.84MVNGAVVTACVEE180 pKa = 4.04LFVLSTRR187 pKa = 11.84RR188 pKa = 11.84DD189 pKa = 3.73CKK191 pKa = 10.21IDD193 pKa = 3.4PVNAIGQDD201 pKa = 3.47VADD204 pKa = 4.36LNRR207 pKa = 11.84YY208 pKa = 8.4NISGTALDD216 pKa = 4.33GQNVAYY222 pKa = 10.0YY223 pKa = 9.71PSDD226 pKa = 3.38SPTFMAHH233 pKa = 4.99FHH235 pKa = 5.76VVQKK239 pKa = 10.99YY240 pKa = 6.88QMCLAPGEE248 pKa = 4.21THH250 pKa = 5.48MQNIYY255 pKa = 10.62VSWEE259 pKa = 3.72KK260 pKa = 10.3MLPTSLASSSTLTTTDD276 pKa = 3.46TFFKK280 pKa = 10.73GYY282 pKa = 8.41TCFLMYY288 pKa = 10.27RR289 pKa = 11.84IVGFPEE295 pKa = 4.28GQVGSSTACASGITDD310 pKa = 3.33CHH312 pKa = 4.12VTIDD316 pKa = 3.02KK317 pKa = 10.5RR318 pKa = 11.84FRR320 pKa = 11.84YY321 pKa = 9.52RR322 pKa = 11.84LLANDD327 pKa = 3.58SKK329 pKa = 11.23HH330 pKa = 6.33YY331 pKa = 10.2EE332 pKa = 4.18VYY334 pKa = 10.93NSVSVPGLPSFVDD347 pKa = 3.72EE348 pKa = 4.23LTGAVMRR355 pKa = 11.84TDD357 pKa = 2.66IFGNVTAAAIQTLIAA372 pKa = 4.62

Molecular weight: 40.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

667

295

372

333.5

37.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.597 ± 1.179

1.949 ± 0.27

5.847 ± 0.594

3.898 ± 1.404

4.798 ± 1.047

6.297 ± 1.636

3.148 ± 0.278

5.247 ± 0.328

6.447 ± 0.212

6.747 ± 1.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.664

3.448 ± 0.253

4.948 ± 0.519

3.598 ± 0.731

6.447 ± 0.436

7.196 ± 1.993

7.196 ± 0.05

7.346 ± 1.225

1.799 ± 0.797

4.648 ± 0.494
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski