Streptomyces zhaozhouensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5537 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286DWY6|A0A286DWY6_9ACTN Iron-containing redox enzyme OS=Streptomyces zhaozhouensis OX=1300267 GN=SAMN06297387_10987 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84TTGRR6 pKa = 11.84LAVSLLTATALLTAGCSSDD25 pKa = 3.82DD26 pKa = 3.74DD27 pKa = 4.31RR28 pKa = 11.84PEE30 pKa = 3.66EE31 pKa = 4.02RR32 pKa = 11.84APDD35 pKa = 3.78ADD37 pKa = 3.9ASDD40 pKa = 4.36GSSTTEE46 pKa = 4.24PPDD49 pKa = 3.94APPLEE54 pKa = 4.58PLSEE58 pKa = 4.15EE59 pKa = 4.29TPEE62 pKa = 4.0EE63 pKa = 3.83LLPYY67 pKa = 10.25YY68 pKa = 9.19EE69 pKa = 4.64QQLNWRR75 pKa = 11.84GCGAAEE81 pKa = 4.05FQCASLTVPLDD92 pKa = 3.71YY93 pKa = 10.92EE94 pKa = 4.72DD95 pKa = 5.54PNPADD100 pKa = 5.54DD101 pKa = 3.84IQLTVTRR108 pKa = 11.84SRR110 pKa = 11.84ATDD113 pKa = 3.09QDD115 pKa = 3.49EE116 pKa = 4.87RR117 pKa = 11.84IGALLMNPGGPGASAVDD134 pKa = 4.38FAQGSAEE141 pKa = 4.58FIYY144 pKa = 10.73ADD146 pKa = 3.85EE147 pKa = 4.11VRR149 pKa = 11.84ARR151 pKa = 11.84YY152 pKa = 10.61DD153 pKa = 3.29MVGLDD158 pKa = 4.25ARR160 pKa = 11.84GTGQSEE166 pKa = 4.03PVSCLEE172 pKa = 3.95GPEE175 pKa = 3.72MDD177 pKa = 5.1AYY179 pKa = 9.85TLVDD183 pKa = 3.73RR184 pKa = 11.84TPDD187 pKa = 3.42DD188 pKa = 3.71EE189 pKa = 6.55AEE191 pKa = 4.08VDD193 pKa = 3.91ALTAAQEE200 pKa = 4.03EE201 pKa = 4.82FARR204 pKa = 11.84GCQEE208 pKa = 3.47GSGALLEE215 pKa = 4.99HH216 pKa = 7.08ISTIEE221 pKa = 3.9SARR224 pKa = 11.84DD225 pKa = 3.31MDD227 pKa = 4.51VLRR230 pKa = 11.84QALGDD235 pKa = 3.76EE236 pKa = 4.18RR237 pKa = 11.84LHH239 pKa = 6.02YY240 pKa = 10.99VGFSYY245 pKa = 8.91GTKK248 pKa = 10.3LGAVYY253 pKa = 10.51AGLYY257 pKa = 6.36PQRR260 pKa = 11.84VGHH263 pKa = 6.66LVLDD267 pKa = 4.13AAMDD271 pKa = 3.93PRR273 pKa = 11.84LDD275 pKa = 3.56TLSTDD280 pKa = 3.51RR281 pKa = 11.84EE282 pKa = 4.09QAGGFEE288 pKa = 4.12TAFRR292 pKa = 11.84AFAEE296 pKa = 4.49DD297 pKa = 4.13CVSGADD303 pKa = 3.71CPLGQEE309 pKa = 4.38SADD312 pKa = 3.85DD313 pKa = 3.73ASQRR317 pKa = 11.84LLDD320 pKa = 4.43FFAAVDD326 pKa = 3.99AEE328 pKa = 4.29PLPSGDD334 pKa = 3.27EE335 pKa = 4.23RR336 pKa = 11.84EE337 pKa = 4.41LTEE340 pKa = 4.33SLATTGVAYY349 pKa = 10.3ALYY352 pKa = 10.67SEE354 pKa = 5.52DD355 pKa = 3.08LWPTLRR361 pKa = 11.84RR362 pKa = 11.84ALAAAIEE369 pKa = 4.57DD370 pKa = 4.17GQGGEE375 pKa = 4.86LLTLADD381 pKa = 4.49SYY383 pKa = 11.8NGRR386 pKa = 11.84TPGGGYY392 pKa = 8.49DD393 pKa = 3.27TDD395 pKa = 3.62MFAFPAISCLDD406 pKa = 3.77SPAGNADD413 pKa = 3.12ADD415 pKa = 3.99AVRR418 pKa = 11.84EE419 pKa = 3.87QLPSYY424 pKa = 10.4EE425 pKa = 4.22EE426 pKa = 4.03ASPTFGADD434 pKa = 3.48FAWATLVCAAWPVEE448 pKa = 4.06PSGGPVSIAAEE459 pKa = 4.23GADD462 pKa = 3.99PILVVGTTRR471 pKa = 11.84DD472 pKa = 3.33PATPYY477 pKa = 10.59AWSEE481 pKa = 3.94GLADD485 pKa = 3.77QLSSGVLLTYY495 pKa = 10.59EE496 pKa = 4.72GDD498 pKa = 2.97GHH500 pKa = 5.3TAYY503 pKa = 10.37GGLSEE508 pKa = 5.6CVDD511 pKa = 3.69STVNDD516 pKa = 3.45YY517 pKa = 11.59LLANTVPDD525 pKa = 5.55DD526 pKa = 3.67GTTCC530 pKa = 4.01

Molecular weight: 56.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286DYQ6|A0A286DYQ6_9ACTN Phosphotransferase enzyme family protein OS=Streptomyces zhaozhouensis OX=1300267 GN=SAMN06297387_112144 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIMANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.37GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5537

0

5537

1918315

28

6744

346.5

37.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.056 ± 0.051

0.72 ± 0.009

5.995 ± 0.03

6.6 ± 0.03

2.623 ± 0.017

9.787 ± 0.032

2.259 ± 0.016

2.607 ± 0.021

1.281 ± 0.018

10.764 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.573 ± 0.013

1.595 ± 0.016

6.468 ± 0.028

2.445 ± 0.02

8.794 ± 0.038

4.75 ± 0.021

5.79 ± 0.026

8.476 ± 0.029

1.54 ± 0.012

1.877 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski