Amino acid dipepetide frequency for Thermobifida phage P1312

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.598AlaAla: 16.598 ± 1.463
0.94AlaCys: 0.94 ± 0.235
7.241AlaAsp: 7.241 ± 0.633
8.37AlaGlu: 8.37 ± 0.775
2.68AlaPhe: 2.68 ± 0.438
10.862AlaGly: 10.862 ± 1.231
2.633AlaHis: 2.633 ± 0.404
2.774AlaIle: 2.774 ± 0.337
2.445AlaLys: 2.445 ± 0.437
9.921AlaLeu: 9.921 ± 0.643
1.881AlaMet: 1.881 ± 0.285
1.411AlaAsn: 1.411 ± 0.243
5.266AlaPro: 5.266 ± 0.533
3.527AlaGln: 3.527 ± 0.415
11.426AlaArg: 11.426 ± 0.816
5.454AlaSer: 5.454 ± 0.618
6.536AlaThr: 6.536 ± 0.521
8.652AlaVal: 8.652 ± 0.776
2.257AlaTrp: 2.257 ± 0.395
2.398AlaTyr: 2.398 ± 0.432
0.0AlaXaa: 0.0 ± 0.0
Cys
1.034CysAla: 1.034 ± 0.283
0.282CysCys: 0.282 ± 0.132
0.47CysAsp: 0.47 ± 0.139
0.282CysGlu: 0.282 ± 0.12
0.047CysPhe: 0.047 ± 0.049
1.129CysGly: 1.129 ± 0.297
0.329CysHis: 0.329 ± 0.135
0.141CysIle: 0.141 ± 0.087
0.0CysLys: 0.0 ± 0.0
0.705CysLeu: 0.705 ± 0.211
0.235CysMet: 0.235 ± 0.095
0.282CysAsn: 0.282 ± 0.123
0.752CysPro: 0.752 ± 0.181
0.282CysGln: 0.282 ± 0.111
1.599CysArg: 1.599 ± 0.314
0.705CysSer: 0.705 ± 0.193
0.611CysThr: 0.611 ± 0.245
0.658CysVal: 0.658 ± 0.207
0.094CysTrp: 0.094 ± 0.062
0.235CysTyr: 0.235 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
6.395AspAla: 6.395 ± 0.487
0.517AspCys: 0.517 ± 0.131
3.762AspAsp: 3.762 ± 0.515
4.843AspGlu: 4.843 ± 0.496
1.081AspPhe: 1.081 ± 0.275
4.608AspGly: 4.608 ± 0.505
1.411AspHis: 1.411 ± 0.27
1.881AspIle: 1.881 ± 0.295
1.223AspLys: 1.223 ± 0.169
5.549AspLeu: 5.549 ± 0.605
0.564AspMet: 0.564 ± 0.185
1.034AspAsn: 1.034 ± 0.233
3.715AspPro: 3.715 ± 0.427
2.539AspGln: 2.539 ± 0.376
5.078AspArg: 5.078 ± 0.669
2.163AspSer: 2.163 ± 0.292
3.15AspThr: 3.15 ± 0.42
4.89AspVal: 4.89 ± 0.537
1.176AspTrp: 1.176 ± 0.283
1.364AspTyr: 1.364 ± 0.268
0.0AspXaa: 0.0 ± 0.0
Glu
8.229GluAla: 8.229 ± 0.894
0.282GluCys: 0.282 ± 0.144
3.621GluAsp: 3.621 ± 0.428
4.984GluGlu: 4.984 ± 0.685
1.317GluPhe: 1.317 ± 0.393
4.984GluGly: 4.984 ± 0.586
1.552GluHis: 1.552 ± 0.236
2.586GluIle: 2.586 ± 0.327
1.928GluLys: 1.928 ± 0.322
6.442GluLeu: 6.442 ± 0.681
1.317GluMet: 1.317 ± 0.264
1.223GluAsn: 1.223 ± 0.245
3.668GluPro: 3.668 ± 0.454
3.433GluGln: 3.433 ± 0.473
6.066GluArg: 6.066 ± 0.725
2.445GluSer: 2.445 ± 0.357
3.291GluThr: 3.291 ± 0.489
4.937GluVal: 4.937 ± 0.505
1.646GluTrp: 1.646 ± 0.288
1.081GluTyr: 1.081 ± 0.27
0.0GluXaa: 0.0 ± 0.0
Phe
2.821PheAla: 2.821 ± 0.523
0.282PheCys: 0.282 ± 0.131
0.893PheAsp: 0.893 ± 0.192
0.987PheGlu: 0.987 ± 0.192
0.564PhePhe: 0.564 ± 0.123
2.727PheGly: 2.727 ± 0.421
0.517PheHis: 0.517 ± 0.161
0.611PheIle: 0.611 ± 0.182
0.376PheLys: 0.376 ± 0.135
1.364PheLeu: 1.364 ± 0.297
0.188PheMet: 0.188 ± 0.104
0.282PheAsn: 0.282 ± 0.137
1.505PhePro: 1.505 ± 0.255
0.752PheGln: 0.752 ± 0.211
1.74PheArg: 1.74 ± 0.289
1.223PheSer: 1.223 ± 0.294
1.27PheThr: 1.27 ± 0.326
1.975PheVal: 1.975 ± 0.326
0.282PheTrp: 0.282 ± 0.105
0.329PheTyr: 0.329 ± 0.111
0.0PheXaa: 0.0 ± 0.0
Gly
8.887GlyAla: 8.887 ± 0.989
1.129GlyCys: 1.129 ± 0.279
5.454GlyAsp: 5.454 ± 0.615
6.489GlyGlu: 6.489 ± 0.663
3.291GlyPhe: 3.291 ± 0.531
9.733GlyGly: 9.733 ± 1.404
2.398GlyHis: 2.398 ± 0.35
2.022GlyIle: 2.022 ± 0.263
1.975GlyLys: 1.975 ± 0.291
9.404GlyLeu: 9.404 ± 0.957
2.163GlyMet: 2.163 ± 0.427
1.081GlyAsn: 1.081 ± 0.243
4.843GlyPro: 4.843 ± 0.801
2.915GlyGln: 2.915 ± 0.346
9.733GlyArg: 9.733 ± 0.848
4.373GlySer: 4.373 ± 0.493
4.138GlyThr: 4.138 ± 0.602
7.806GlyVal: 7.806 ± 0.767
2.398GlyTrp: 2.398 ± 0.465
1.928GlyTyr: 1.928 ± 0.366
0.0GlyXaa: 0.0 ± 0.0
His
2.868HisAla: 2.868 ± 0.443
0.047HisCys: 0.047 ± 0.046
1.646HisAsp: 1.646 ± 0.373
1.505HisGlu: 1.505 ± 0.315
0.329HisPhe: 0.329 ± 0.101
2.633HisGly: 2.633 ± 0.406
1.129HisHis: 1.129 ± 0.329
1.223HisIle: 1.223 ± 0.206
0.658HisLys: 0.658 ± 0.169
2.633HisLeu: 2.633 ± 0.365
0.329HisMet: 0.329 ± 0.121
0.329HisAsn: 0.329 ± 0.119
1.881HisPro: 1.881 ± 0.382
1.081HisGln: 1.081 ± 0.325
2.727HisArg: 2.727 ± 0.528
1.317HisSer: 1.317 ± 0.224
1.505HisThr: 1.505 ± 0.307
1.834HisVal: 1.834 ± 0.311
0.799HisTrp: 0.799 ± 0.192
0.611HisTyr: 0.611 ± 0.161
0.0HisXaa: 0.0 ± 0.0
Ile
3.95IleAla: 3.95 ± 0.348
0.235IleCys: 0.235 ± 0.1
2.351IleAsp: 2.351 ± 0.298
1.928IleGlu: 1.928 ± 0.514
0.376IlePhe: 0.376 ± 0.13
2.398IleGly: 2.398 ± 0.355
1.034IleHis: 1.034 ± 0.202
1.364IleIle: 1.364 ± 0.275
0.752IleLys: 0.752 ± 0.211
2.539IleLeu: 2.539 ± 0.33
0.47IleMet: 0.47 ± 0.147
0.987IleAsn: 0.987 ± 0.193
2.257IlePro: 2.257 ± 0.332
1.081IleGln: 1.081 ± 0.271
3.715IleArg: 3.715 ± 0.463
2.022IleSer: 2.022 ± 0.299
2.727IleThr: 2.727 ± 0.395
2.774IleVal: 2.774 ± 0.35
0.611IleTrp: 0.611 ± 0.172
0.423IleTyr: 0.423 ± 0.152
0.0IleXaa: 0.0 ± 0.0
Lys
2.304LysAla: 2.304 ± 0.416
0.235LysCys: 0.235 ± 0.11
0.705LysAsp: 0.705 ± 0.179
1.27LysGlu: 1.27 ± 0.235
0.282LysPhe: 0.282 ± 0.186
1.646LysGly: 1.646 ± 0.325
0.658LysHis: 0.658 ± 0.152
1.129LysIle: 1.129 ± 0.247
0.893LysLys: 0.893 ± 0.235
1.881LysLeu: 1.881 ± 0.341
0.376LysMet: 0.376 ± 0.178
0.705LysAsn: 0.705 ± 0.162
1.599LysPro: 1.599 ± 0.27
1.364LysGln: 1.364 ± 0.234
2.68LysArg: 2.68 ± 0.351
1.176LysSer: 1.176 ± 0.215
1.646LysThr: 1.646 ± 0.305
1.505LysVal: 1.505 ± 0.294
0.423LysTrp: 0.423 ± 0.16
0.376LysTyr: 0.376 ± 0.129
0.0LysXaa: 0.0 ± 0.0
Leu
10.157LeuAla: 10.157 ± 0.938
0.705LeuCys: 0.705 ± 0.175
5.219LeuAsp: 5.219 ± 0.35
5.643LeuGlu: 5.643 ± 0.641
1.74LeuPhe: 1.74 ± 0.301
8.323LeuGly: 8.323 ± 0.814
2.586LeuHis: 2.586 ± 0.375
3.197LeuIle: 3.197 ± 0.394
1.834LeuLys: 1.834 ± 0.265
7.523LeuLeu: 7.523 ± 0.849
1.364LeuMet: 1.364 ± 0.29
1.928LeuAsn: 1.928 ± 0.263
5.831LeuPro: 5.831 ± 0.484
2.492LeuGln: 2.492 ± 0.416
8.699LeuArg: 8.699 ± 0.695
4.749LeuSer: 4.749 ± 0.437
5.501LeuThr: 5.501 ± 0.592
7.994LeuVal: 7.994 ± 0.52
0.94LeuTrp: 0.94 ± 0.265
1.881LeuTyr: 1.881 ± 0.358
0.0LeuXaa: 0.0 ± 0.0
Met
2.116MetAla: 2.116 ± 0.479
0.282MetCys: 0.282 ± 0.109
1.034MetAsp: 1.034 ± 0.24
0.611MetGlu: 0.611 ± 0.171
0.282MetPhe: 0.282 ± 0.09
1.411MetGly: 1.411 ± 0.293
0.47MetHis: 0.47 ± 0.146
0.423MetIle: 0.423 ± 0.153
0.376MetLys: 0.376 ± 0.119
1.317MetLeu: 1.317 ± 0.265
0.376MetMet: 0.376 ± 0.117
0.329MetAsn: 0.329 ± 0.125
0.846MetPro: 0.846 ± 0.229
0.423MetGln: 0.423 ± 0.121
1.787MetArg: 1.787 ± 0.362
1.176MetSer: 1.176 ± 0.26
1.552MetThr: 1.552 ± 0.28
1.458MetVal: 1.458 ± 0.246
0.094MetTrp: 0.094 ± 0.061
0.188MetTyr: 0.188 ± 0.088
0.0MetXaa: 0.0 ± 0.0
Asn
1.458AsnAla: 1.458 ± 0.231
0.047AsnCys: 0.047 ± 0.044
1.034AsnAsp: 1.034 ± 0.257
0.987AsnGlu: 0.987 ± 0.231
0.376AsnPhe: 0.376 ± 0.139
1.599AsnGly: 1.599 ± 0.319
0.611AsnHis: 0.611 ± 0.153
0.329AsnIle: 0.329 ± 0.096
0.282AsnLys: 0.282 ± 0.121
1.552AsnLeu: 1.552 ± 0.287
0.094AsnMet: 0.094 ± 0.066
0.564AsnAsn: 0.564 ± 0.188
2.304AsnPro: 2.304 ± 0.327
0.846AsnGln: 0.846 ± 0.171
2.398AsnArg: 2.398 ± 0.286
1.081AsnSer: 1.081 ± 0.23
1.787AsnThr: 1.787 ± 0.322
1.176AsnVal: 1.176 ± 0.202
0.423AsnTrp: 0.423 ± 0.17
0.188AsnTyr: 0.188 ± 0.086
0.0AsnXaa: 0.0 ± 0.0
Pro
7.335ProAla: 7.335 ± 0.74
0.47ProCys: 0.47 ± 0.158
3.997ProAsp: 3.997 ± 0.52
4.044ProGlu: 4.044 ± 0.448
1.27ProPhe: 1.27 ± 0.318
5.784ProGly: 5.784 ± 0.69
2.257ProHis: 2.257 ± 0.422
1.787ProIle: 1.787 ± 0.28
1.317ProLys: 1.317 ± 0.263
5.878ProLeu: 5.878 ± 0.684
1.081ProMet: 1.081 ± 0.197
1.646ProAsn: 1.646 ± 0.35
5.219ProPro: 5.219 ± 0.685
2.022ProGln: 2.022 ± 0.347
5.313ProArg: 5.313 ± 0.581
2.962ProSer: 2.962 ± 0.443
4.044ProThr: 4.044 ± 0.528
6.019ProVal: 6.019 ± 0.604
0.987ProTrp: 0.987 ± 0.241
1.034ProTyr: 1.034 ± 0.173
0.0ProXaa: 0.0 ± 0.0
Gln
4.42GlnAla: 4.42 ± 0.544
0.282GlnCys: 0.282 ± 0.122
1.129GlnAsp: 1.129 ± 0.223
2.022GlnGlu: 2.022 ± 0.321
0.94GlnPhe: 0.94 ± 0.204
3.15GlnGly: 3.15 ± 0.33
0.893GlnHis: 0.893 ± 0.287
1.364GlnIle: 1.364 ± 0.264
0.799GlnLys: 0.799 ± 0.181
3.339GlnLeu: 3.339 ± 0.466
0.47GlnMet: 0.47 ± 0.163
0.329GlnAsn: 0.329 ± 0.115
3.386GlnPro: 3.386 ± 0.506
2.539GlnGln: 2.539 ± 0.311
3.903GlnArg: 3.903 ± 0.582
1.787GlnSer: 1.787 ± 0.402
1.74GlnThr: 1.74 ± 0.347
3.103GlnVal: 3.103 ± 0.459
0.799GlnTrp: 0.799 ± 0.198
0.423GlnTyr: 0.423 ± 0.148
0.0GlnXaa: 0.0 ± 0.0
Arg
9.639ArgAla: 9.639 ± 0.77
1.505ArgCys: 1.505 ± 0.318
4.608ArgAsp: 4.608 ± 0.467
5.36ArgGlu: 5.36 ± 0.629
1.928ArgPhe: 1.928 ± 0.358
9.357ArgGly: 9.357 ± 0.841
3.009ArgHis: 3.009 ± 0.433
3.997ArgIle: 3.997 ± 0.394
2.915ArgLys: 2.915 ± 0.385
9.874ArgLeu: 9.874 ± 1.025
1.975ArgMet: 1.975 ± 0.352
2.351ArgAsn: 2.351 ± 0.381
6.536ArgPro: 6.536 ± 0.844
3.056ArgGln: 3.056 ± 0.353
11.144ArgArg: 11.144 ± 0.934
5.031ArgSer: 5.031 ± 0.921
5.407ArgThr: 5.407 ± 0.697
7.57ArgVal: 7.57 ± 0.661
2.445ArgTrp: 2.445 ± 0.356
2.304ArgTyr: 2.304 ± 0.379
0.0ArgXaa: 0.0 ± 0.0
Ser
4.937SerAla: 4.937 ± 0.565
0.47SerCys: 0.47 ± 0.16
2.304SerAsp: 2.304 ± 0.31
3.009SerGlu: 3.009 ± 0.449
1.223SerPhe: 1.223 ± 0.323
5.69SerGly: 5.69 ± 1.103
1.129SerHis: 1.129 ± 0.227
1.646SerIle: 1.646 ± 0.314
1.411SerLys: 1.411 ± 0.312
3.997SerLeu: 3.997 ± 0.389
1.034SerMet: 1.034 ± 0.178
0.893SerAsn: 0.893 ± 0.258
3.903SerPro: 3.903 ± 0.416
1.223SerGln: 1.223 ± 0.223
4.984SerArg: 4.984 ± 0.575
3.95SerSer: 3.95 ± 0.43
3.621SerThr: 3.621 ± 0.394
3.621SerVal: 3.621 ± 0.352
0.846SerTrp: 0.846 ± 0.229
1.176SerTyr: 1.176 ± 0.271
0.0SerXaa: 0.0 ± 0.0
Thr
5.596ThrAla: 5.596 ± 0.586
1.081ThrCys: 1.081 ± 0.236
2.633ThrAsp: 2.633 ± 0.383
3.95ThrGlu: 3.95 ± 0.529
0.658ThrPhe: 0.658 ± 0.194
5.125ThrGly: 5.125 ± 0.565
1.317ThrHis: 1.317 ± 0.234
3.15ThrIle: 3.15 ± 0.45
1.129ThrLys: 1.129 ± 0.312
4.655ThrLeu: 4.655 ± 0.43
1.034ThrMet: 1.034 ± 0.221
1.034ThrAsn: 1.034 ± 0.274
4.702ThrPro: 4.702 ± 0.691
2.351ThrGln: 2.351 ± 0.26
5.454ThrArg: 5.454 ± 0.822
3.762ThrSer: 3.762 ± 0.61
4.843ThrThr: 4.843 ± 0.597
4.984ThrVal: 4.984 ± 0.459
0.94ThrTrp: 0.94 ± 0.232
1.458ThrTyr: 1.458 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
10.157ValAla: 10.157 ± 0.68
0.752ValCys: 0.752 ± 0.222
6.489ValAsp: 6.489 ± 0.485
6.348ValGlu: 6.348 ± 0.582
1.787ValPhe: 1.787 ± 0.227
7.523ValGly: 7.523 ± 0.661
1.928ValHis: 1.928 ± 0.489
2.868ValIle: 2.868 ± 0.337
1.458ValLys: 1.458 ± 0.321
5.972ValLeu: 5.972 ± 0.462
0.94ValMet: 0.94 ± 0.22
1.74ValAsn: 1.74 ± 0.29
4.091ValPro: 4.091 ± 0.488
2.821ValGln: 2.821 ± 0.282
7.476ValArg: 7.476 ± 0.606
4.373ValSer: 4.373 ± 0.332
3.997ValThr: 3.997 ± 0.427
7.9ValVal: 7.9 ± 0.579
1.552ValTrp: 1.552 ± 0.304
1.787ValTyr: 1.787 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
2.022TrpAla: 2.022 ± 0.421
0.235TrpCys: 0.235 ± 0.148
1.223TrpAsp: 1.223 ± 0.269
1.176TrpGlu: 1.176 ± 0.243
0.423TrpPhe: 0.423 ± 0.156
1.881TrpGly: 1.881 ± 0.323
0.705TrpHis: 0.705 ± 0.194
0.94TrpIle: 0.94 ± 0.277
0.423TrpLys: 0.423 ± 0.144
2.492TrpLeu: 2.492 ± 0.357
0.47TrpMet: 0.47 ± 0.128
0.423TrpAsn: 0.423 ± 0.144
0.752TrpPro: 0.752 ± 0.185
1.034TrpGln: 1.034 ± 0.208
1.458TrpArg: 1.458 ± 0.238
0.517TrpSer: 0.517 ± 0.162
1.129TrpThr: 1.129 ± 0.229
1.458TrpVal: 1.458 ± 0.294
0.799TrpTrp: 0.799 ± 0.21
0.376TrpTyr: 0.376 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.351TyrAla: 2.351 ± 0.36
0.094TyrCys: 0.094 ± 0.072
1.129TyrAsp: 1.129 ± 0.214
1.223TyrGlu: 1.223 ± 0.314
0.094TyrPhe: 0.094 ± 0.067
1.599TyrGly: 1.599 ± 0.282
0.517TyrHis: 0.517 ± 0.166
0.564TyrIle: 0.564 ± 0.166
0.658TyrLys: 0.658 ± 0.219
1.27TyrLeu: 1.27 ± 0.3
0.141TyrMet: 0.141 ± 0.092
0.564TyrAsn: 0.564 ± 0.153
1.411TyrPro: 1.411 ± 0.272
1.034TyrGln: 1.034 ± 0.251
2.633TyrArg: 2.633 ± 0.405
0.799TyrSer: 0.799 ± 0.218
1.411TyrThr: 1.411 ± 0.263
1.646TyrVal: 1.646 ± 0.31
0.423TyrTrp: 0.423 ± 0.154
0.423TyrTyr: 0.423 ± 0.149
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (21268 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski