Dickeya phage vB_DsoM_JA33

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Salmondvirus; unclassified Salmondvirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384ZZG0|A0A384ZZG0_9CAUD Uncharacterized protein OS=Dickeya phage vB_DsoM_JA33 OX=2283032 GN=JA33_260 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.76SMTRR6 pKa = 11.84VISILAGTDD15 pKa = 3.36EE16 pKa = 4.65LSEE19 pKa = 4.09AFISVLHH26 pKa = 6.36KK27 pKa = 10.91ADD29 pKa = 2.97VDD31 pKa = 4.28VNVDD35 pKa = 2.84IDD37 pKa = 4.96RR38 pKa = 11.84VILLNDD44 pKa = 3.49YY45 pKa = 11.04GSATLVPNDD54 pKa = 3.38NAVEE58 pKa = 4.1IEE60 pKa = 4.01SSNLHH65 pKa = 5.88GSRR68 pKa = 11.84VIINDD73 pKa = 3.63PVSALLFALYY83 pKa = 10.32RR84 pKa = 11.84LNLRR88 pKa = 11.84LDD90 pKa = 3.76VEE92 pKa = 5.19DD93 pKa = 4.21EE94 pKa = 4.21DD95 pKa = 4.95QIGVLPTGYY104 pKa = 9.38PVLPEE109 pKa = 4.07PRR111 pKa = 11.84CTFADD116 pKa = 4.01HH117 pKa = 6.5SAKK120 pKa = 10.52AYY122 pKa = 9.36TKK124 pKa = 10.44EE125 pKa = 3.95QMLEE129 pKa = 3.93YY130 pKa = 10.79ARR132 pKa = 11.84DD133 pKa = 3.35AVVRR137 pKa = 11.84FTGMAPFEE145 pKa = 4.43IEE147 pKa = 4.0SDD149 pKa = 3.62PVSFDD154 pKa = 4.37DD155 pKa = 4.14MYY157 pKa = 11.41KK158 pKa = 8.93EE159 pKa = 5.25HH160 pKa = 6.96AVEE163 pKa = 4.09WCDD166 pKa = 3.49HH167 pKa = 6.61CGGQIGDD174 pKa = 4.34DD175 pKa = 3.99CSCEE179 pKa = 4.2DD180 pKa = 3.96GEE182 pKa = 4.68ADD184 pKa = 3.61EE185 pKa = 6.6DD186 pKa = 4.05EE187 pKa = 6.05DD188 pKa = 5.68EE189 pKa = 4.99DD190 pKa = 5.4EE191 pKa = 4.28EE192 pKa = 5.43WEE194 pKa = 4.8DD195 pKa = 5.15ADD197 pKa = 5.83CEE199 pKa = 4.53HH200 pKa = 7.14CGCTNCDD207 pKa = 3.22CDD209 pKa = 4.6IDD211 pKa = 4.7EE212 pKa = 5.2DD213 pKa = 4.03EE214 pKa = 5.41CPRR217 pKa = 11.84CHH219 pKa = 7.78SSSCQCLPDD228 pKa = 4.29FDD230 pKa = 4.69GQEE233 pKa = 4.72DD234 pKa = 4.06PDD236 pKa = 3.56QRR238 pKa = 11.84KK239 pKa = 8.93

Molecular weight: 26.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384ZZF4|A0A384ZZF4_9CAUD Uncharacterized protein OS=Dickeya phage vB_DsoM_JA33 OX=2283032 GN=JA33_213 PE=4 SV=1
MM1 pKa = 7.9KK2 pKa = 10.06FRR4 pKa = 11.84KK5 pKa = 9.79LLTRR9 pKa = 11.84NKK11 pKa = 9.64NRR13 pKa = 11.84RR14 pKa = 11.84SISASCFIGRR24 pKa = 11.84LFFEE28 pKa = 4.44IGVGRR33 pKa = 11.84EE34 pKa = 3.36LWYY37 pKa = 8.43FTRR40 pKa = 11.84FGKK43 pKa = 10.53NNDD46 pKa = 2.75HH47 pKa = 7.27RR48 pKa = 11.84IYY50 pKa = 10.7HH51 pKa = 6.52LFHH54 pKa = 7.53VGVTRR59 pKa = 11.84LVNPSGEE66 pKa = 3.89AHH68 pKa = 6.47TDD70 pKa = 3.16VLVISFLLLNIKK82 pKa = 9.42FGWMTIKK89 pKa = 10.81DD90 pKa = 3.66KK91 pKa = 11.52

Molecular weight: 10.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

321

0

321

79750

33

1944

248.4

27.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.619 ± 0.148

1.105 ± 0.063

6.588 ± 0.145

6.83 ± 0.249

4.324 ± 0.082

5.949 ± 0.154

1.832 ± 0.069

6.13 ± 0.123

6.884 ± 0.216

8.221 ± 0.148

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.072

4.816 ± 0.103

4.217 ± 0.107

3.387 ± 0.074

5.32 ± 0.114

6.961 ± 0.11

5.797 ± 0.146

6.959 ± 0.109

1.05 ± 0.049

3.52 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski