Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geobacter; Geobacter metallireducens

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3502 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q39Q13|Q39Q13_GEOMG Lipoprotein putative OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=Gmet_3452 PE=4 SV=1
MM1 pKa = 7.29TKK3 pKa = 10.71APLVDD8 pKa = 3.65KK9 pKa = 10.08DD10 pKa = 3.73VCISCGLCVDD20 pKa = 5.13NIPDD24 pKa = 3.57VFRR27 pKa = 11.84FDD29 pKa = 4.86DD30 pKa = 4.31DD31 pKa = 4.21NKK33 pKa = 11.32AEE35 pKa = 4.29CFDD38 pKa = 4.58PNGAAEE44 pKa = 5.58DD45 pKa = 4.37VIQSDD50 pKa = 5.32AIDD53 pKa = 4.18ACPVSCISWSTDD65 pKa = 2.77PDD67 pKa = 3.89YY68 pKa = 11.56LKK70 pKa = 11.18SFAA73 pKa = 4.93

Molecular weight: 7.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q39PS3|Q39PS3_GEOMG Pentapeptide repeat domain protein OS=Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15) OX=269799 GN=Gmet_3546 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNTSRR12 pKa = 11.84KK13 pKa = 7.36RR14 pKa = 11.84THH16 pKa = 6.11GFRR19 pKa = 11.84VRR21 pKa = 11.84MSTKK25 pKa = 10.14NGRR28 pKa = 11.84LVIKK32 pKa = 10.32RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.73RR41 pKa = 11.84LAVTIAGKK49 pKa = 10.28

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3502

0

3502

1188476

20

3507

339.4

37.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.842 ± 0.048

1.247 ± 0.02

5.24 ± 0.03

6.658 ± 0.055

4.026 ± 0.03

8.415 ± 0.04

2.041 ± 0.02

5.761 ± 0.033

4.677 ± 0.039

9.998 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.018

3.125 ± 0.034

4.72 ± 0.029

2.846 ± 0.023

6.613 ± 0.047

5.431 ± 0.036

5.528 ± 0.061

7.602 ± 0.036

1.039 ± 0.016

2.743 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski