Tolumonas auensis (strain DSM 9187 / TA4)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales; Aeromonadaceae; Tolumonas; Tolumonas auensis

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4LAT8|C4LAT8_TOLAT Antitoxin OS=Tolumonas auensis (strain DSM 9187 / TA4) OX=595494 GN=Tola_0663 PE=3 SV=1
MM1 pKa = 7.35EE2 pKa = 4.64NQWAICHH9 pKa = 5.96VDD11 pKa = 3.09SSFDD15 pKa = 3.48ASLLGNRR22 pKa = 11.84GSQWHH27 pKa = 6.23WFHH30 pKa = 7.75SRR32 pKa = 11.84DD33 pKa = 4.48DD34 pKa = 4.48LIEE37 pKa = 4.11YY38 pKa = 10.14LLNEE42 pKa = 4.72YY43 pKa = 10.12IYY45 pKa = 11.07LLADD49 pKa = 3.58AGEE52 pKa = 4.21LDD54 pKa = 3.85EE55 pKa = 5.03EE56 pKa = 4.44QAEE59 pKa = 4.48SARR62 pKa = 11.84EE63 pKa = 3.75RR64 pKa = 11.84FEE66 pKa = 5.02LLIEE70 pKa = 4.04QSHH73 pKa = 7.46DD74 pKa = 3.42DD75 pKa = 3.63TVLAEE80 pKa = 4.08QLNDD84 pKa = 3.36LTDD87 pKa = 3.43SLRR90 pKa = 11.84RR91 pKa = 11.84IVWFGTLAQLAEE103 pKa = 4.48IDD105 pKa = 4.99DD106 pKa = 4.22EE107 pKa = 4.45FANALRR113 pKa = 11.84SYY115 pKa = 10.1FWNDD119 pKa = 2.95YY120 pKa = 10.63GDD122 pKa = 4.83NEE124 pKa = 5.21DD125 pKa = 4.5DD126 pKa = 4.22PEE128 pKa = 5.24AAVPEE133 pKa = 4.25EE134 pKa = 4.31LWPEE138 pKa = 3.78FADD141 pKa = 4.56TIDD144 pKa = 3.37EE145 pKa = 4.14FLIEE149 pKa = 4.89GEE151 pKa = 4.44YY152 pKa = 10.84CC153 pKa = 3.79

Molecular weight: 17.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|C4LEG3|AROA_TOLAT 3-phosphoshikimate 1-carboxyvinyltransferase OS=Tolumonas auensis (strain DSM 9187 / TA4) OX=595494 GN=aroA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84ARR14 pKa = 11.84NHH16 pKa = 5.51GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.05VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.24GRR39 pKa = 11.84ARR41 pKa = 11.84LTTVV45 pKa = 2.7

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3126

0

3126

1023253

32

1656

327.3

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.451 ± 0.053

1.128 ± 0.016

5.33 ± 0.035

5.87 ± 0.042

3.884 ± 0.03

7.058 ± 0.039

2.391 ± 0.02

6.12 ± 0.036

4.602 ± 0.039

11.089 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.02

3.685 ± 0.025

4.37 ± 0.022

4.868 ± 0.051

5.247 ± 0.037

5.952 ± 0.03

5.198 ± 0.029

6.838 ± 0.035

1.362 ± 0.021

2.913 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski