Avon-Heathcote Estuary associated circular virus 8

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IAY1|A0A0C5IAY1_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 8 OX=1618259 PE=4 SV=1
MM1 pKa = 7.54YY2 pKa = 9.93MGDD5 pKa = 3.48SQLAVWDD12 pKa = 4.3FRR14 pKa = 11.84YY15 pKa = 10.11NASTFKK21 pKa = 11.0SEE23 pKa = 4.17SDD25 pKa = 3.19LKK27 pKa = 11.29AVLKK31 pKa = 10.84GIAKK35 pKa = 9.41KK36 pKa = 9.51WVFQLEE42 pKa = 4.37RR43 pKa = 11.84GDD45 pKa = 3.68EE46 pKa = 4.55GYY48 pKa = 10.51EE49 pKa = 4.0HH50 pKa = 6.2YY51 pKa = 10.05QGRR54 pKa = 11.84MSLIKK59 pKa = 10.28GRR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 10.2GEE65 pKa = 3.59KK66 pKa = 9.89HH67 pKa = 6.11ILLKK71 pKa = 10.68LFKK74 pKa = 8.77EE75 pKa = 4.57TPPNYY80 pKa = 10.33LEE82 pKa = 4.38PTSKK86 pKa = 9.73TEE88 pKa = 3.9ATKK91 pKa = 11.13GDD93 pKa = 3.7ALYY96 pKa = 10.69VIKK99 pKa = 10.48EE100 pKa = 4.09DD101 pKa = 3.94TRR103 pKa = 11.84LKK105 pKa = 11.0GPWTDD110 pKa = 3.06KK111 pKa = 11.59DD112 pKa = 3.76EE113 pKa = 4.8VKK115 pKa = 10.97VEE117 pKa = 4.06TTQLKK122 pKa = 9.9IFKK125 pKa = 9.9SLEE128 pKa = 3.41LRR130 pKa = 11.84PYY132 pKa = 9.29QRR134 pKa = 11.84KK135 pKa = 9.49LEE137 pKa = 4.11EE138 pKa = 3.82FARR141 pKa = 11.84TFDD144 pKa = 3.35MRR146 pKa = 11.84KK147 pKa = 9.1IDD149 pKa = 4.86IIYY152 pKa = 10.37DD153 pKa = 4.05PIGDD157 pKa = 3.9LGKK160 pKa = 10.69SLFSEE165 pKa = 3.62WMEE168 pKa = 3.94YY169 pKa = 10.63EE170 pKa = 4.18GLAEE174 pKa = 4.95EE175 pKa = 4.3IPPYY179 pKa = 10.86RR180 pKa = 11.84LMDD183 pKa = 6.69DD184 pKa = 3.62IFQWVATRR192 pKa = 11.84PIKK195 pKa = 10.07PCYY198 pKa = 8.94IVDD201 pKa = 3.69MPRR204 pKa = 11.84GMKK207 pKa = 9.53KK208 pKa = 10.41DD209 pKa = 3.5KK210 pKa = 11.36LGDD213 pKa = 3.75FYY215 pKa = 11.81SGLEE219 pKa = 4.05VIKK222 pKa = 11.18NGVAYY227 pKa = 10.04DD228 pKa = 3.46KK229 pKa = 10.61RR230 pKa = 11.84YY231 pKa = 9.74NATKK235 pKa = 10.15IRR237 pKa = 11.84FNRR240 pKa = 11.84PRR242 pKa = 11.84VFVFTNTLPEE252 pKa = 4.13FSLMSKK258 pKa = 10.25DD259 pKa = 3.21RR260 pKa = 11.84WNVWEE265 pKa = 4.04VQEE268 pKa = 5.96DD269 pKa = 3.99YY270 pKa = 11.25DD271 pKa = 3.83ILLRR275 pKa = 11.84NEE277 pKa = 3.96MGTLSMKK284 pKa = 10.6

Molecular weight: 33.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IAY1|A0A0C5IAY1_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 8 OX=1618259 PE=4 SV=1
MM1 pKa = 7.74PKK3 pKa = 10.29YY4 pKa = 10.15SGPRR8 pKa = 11.84MPSGRR13 pKa = 11.84YY14 pKa = 6.88YY15 pKa = 11.02SKK17 pKa = 10.95RR18 pKa = 11.84KK19 pKa = 9.48AGRR22 pKa = 11.84PKK24 pKa = 10.58GSKK27 pKa = 9.76NKK29 pKa = 9.71PKK31 pKa = 10.67AKK33 pKa = 10.07RR34 pKa = 11.84GGLNKK39 pKa = 10.13TEE41 pKa = 4.24VKK43 pKa = 7.69QTRR46 pKa = 11.84AIAKK50 pKa = 9.71RR51 pKa = 11.84VINSNAEE58 pKa = 3.81SKK60 pKa = 10.94YY61 pKa = 10.32FDD63 pKa = 3.93VRR65 pKa = 11.84TIDD68 pKa = 3.41QLSGSGGVGIVPTPARR84 pKa = 11.84LAYY87 pKa = 7.85TQLYY91 pKa = 8.2VQGFAVGEE99 pKa = 4.07NTIGGTALTYY109 pKa = 9.62GQTVIEE115 pKa = 5.03PINHH119 pKa = 5.22GRR121 pKa = 11.84IHH123 pKa = 7.19PSGNSDD129 pKa = 3.29NQNLEE134 pKa = 3.67GQYY137 pKa = 11.59AMPSLSITTFDD148 pKa = 3.18IQRR151 pKa = 11.84VIQTSDD157 pKa = 3.08LTLDD161 pKa = 4.26LARR164 pKa = 11.84NQTPFMVRR172 pKa = 11.84VLRR175 pKa = 11.84LVPRR179 pKa = 11.84PQKK182 pKa = 10.79GSSQGLDD189 pKa = 3.33PANDD193 pKa = 3.39AFVNEE198 pKa = 4.74SNQEE202 pKa = 3.84TGVADD207 pKa = 3.43SGFEE211 pKa = 4.02HH212 pKa = 6.53YY213 pKa = 10.45QLLFLKK219 pKa = 10.48ANSKK223 pKa = 10.17KK224 pKa = 10.55YY225 pKa = 8.68QVKK228 pKa = 9.8QDD230 pKa = 3.21FRR232 pKa = 11.84MVLNPPLSTTEE243 pKa = 3.65VAMQGNAGSTAYY255 pKa = 10.3SVANISNNFHH265 pKa = 6.24RR266 pKa = 11.84QMTFKK271 pKa = 10.84HH272 pKa = 6.83DD273 pKa = 3.12IGKK276 pKa = 9.92KK277 pKa = 9.98LFYY280 pKa = 10.42DD281 pKa = 3.87NPQSRR286 pKa = 11.84SHH288 pKa = 6.31PTDD291 pKa = 2.87GFKK294 pKa = 11.18NEE296 pKa = 4.97FILFHH301 pKa = 6.96IIPLGTANDD310 pKa = 4.06GGILPTSVRR319 pKa = 11.84IAAKK323 pKa = 10.13AVSTFKK329 pKa = 11.11DD330 pKa = 3.8FF331 pKa = 4.19

Molecular weight: 36.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

615

284

331

307.5

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.528 ± 1.045

0.163 ± 0.12

5.854 ± 0.972

5.528 ± 1.844

4.878 ± 0.033

7.48 ± 0.943

1.463 ± 0.479

5.528 ± 0.066

8.618 ± 1.228

7.805 ± 0.852

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.927 ± 0.597

4.878 ± 1.301

5.366 ± 0.498

4.228 ± 1.112

6.179 ± 0.323

6.341 ± 1.113

6.179 ± 0.566

5.528 ± 0.378

1.138 ± 0.837

4.39 ± 0.563
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski