Leporid alphaherpesvirus 4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9R053|J9R053_9ALPH DNA packaging tegument protein UL17 OS=Leporid alphaherpesvirus 4 OX=481315 GN=UL17a PE=4 SV=1
MM1 pKa = 7.51VSLTSDD7 pKa = 3.25EE8 pKa = 4.3FDD10 pKa = 3.56QVDD13 pKa = 3.92ISPTSSDD20 pKa = 2.87EE21 pKa = 4.5FYY23 pKa = 10.98LVPAEE28 pKa = 4.04MQKK31 pKa = 10.99KK32 pKa = 8.77YY33 pKa = 6.99MTGRR37 pKa = 11.84GYY39 pKa = 9.14TRR41 pKa = 11.84IEE43 pKa = 4.08SSPEE47 pKa = 3.63KK48 pKa = 10.23PADD51 pKa = 3.98RR52 pKa = 11.84PSSSPVSPQPP62 pKa = 3.37

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9QYL4|J9QYL4_9ALPH Uracil-DNA glycosylase OS=Leporid alphaherpesvirus 4 OX=481315 GN=UL2 PE=3 SV=1
MM1 pKa = 7.5PAHH4 pKa = 6.95RR5 pKa = 11.84PCAAVRR11 pKa = 11.84EE12 pKa = 4.46AAPVTPNPVLRR23 pKa = 11.84GTRR26 pKa = 11.84HH27 pKa = 4.67SRR29 pKa = 11.84VYY31 pKa = 10.33RR32 pKa = 11.84VGPAEE37 pKa = 3.96GVPFARR43 pKa = 11.84HH44 pKa = 4.97TSASHH49 pKa = 6.78RR50 pKa = 11.84PPAPLPARR58 pKa = 11.84LTAGLFDD65 pKa = 4.38TLNLTAPRR73 pKa = 11.84VGDD76 pKa = 3.69PGRR79 pKa = 11.84VQVIVAEE86 pKa = 4.33RR87 pKa = 11.84DD88 pKa = 3.1RR89 pKa = 11.84LTRR92 pKa = 11.84DD93 pKa = 3.11VPSNDD98 pKa = 2.43EE99 pKa = 4.27RR100 pKa = 11.84IARR103 pKa = 11.84RR104 pKa = 11.84CVRR107 pKa = 11.84GPPHH111 pKa = 6.95PGGPGTAAISCASPRR126 pKa = 11.84RR127 pKa = 11.84AGGRR131 pKa = 11.84VPIATGPPSGGGVGSLRR148 pKa = 11.84RR149 pKa = 11.84GGGSLGTSRR158 pKa = 11.84RR159 pKa = 11.84HH160 pKa = 4.7SRR162 pKa = 11.84LVRR165 pKa = 11.84GVASAAPGASSAPRR179 pKa = 11.84GPPPHH184 pKa = 6.88SPWAPPPHH192 pKa = 6.64RR193 pKa = 11.84RR194 pKa = 11.84LPPVRR199 pKa = 11.84PLPSRR204 pKa = 11.84GARR207 pKa = 11.84RR208 pKa = 11.84ARR210 pKa = 11.84KK211 pKa = 8.44PPPAARR217 pKa = 11.84RR218 pKa = 11.84PARR221 pKa = 11.84RR222 pKa = 11.84GRR224 pKa = 11.84PGGGARR230 pKa = 11.84ARR232 pKa = 11.84IPPGARR238 pKa = 11.84PDD240 pKa = 3.74PAPPPRR246 pKa = 11.84APPRR250 pKa = 11.84VSRR253 pKa = 11.84GPPPGARR260 pKa = 11.84GVAQSSRR267 pKa = 11.84GRR269 pKa = 11.84GRR271 pKa = 11.84GAQTEE276 pKa = 4.43EE277 pKa = 4.02SRR279 pKa = 11.84SPCSCEE285 pKa = 3.6GQAALLGPSAPPRR298 pKa = 11.84KK299 pKa = 9.81GPPPRR304 pKa = 11.84GARR307 pKa = 11.84APRR310 pKa = 11.84PSAAPPPTGPWRR322 pKa = 11.84GGPRR326 pKa = 11.84RR327 pKa = 11.84TPSGCSPAAAGPRR340 pKa = 11.84PPPARR345 pKa = 11.84AARR348 pKa = 11.84PRR350 pKa = 11.84GPARR354 pKa = 11.84GARR357 pKa = 11.84PRR359 pKa = 11.84RR360 pKa = 11.84TRR362 pKa = 11.84AGPRR366 pKa = 11.84ARR368 pKa = 11.84PRR370 pKa = 11.84AISARR375 pKa = 11.84RR376 pKa = 11.84RR377 pKa = 11.84AAGRR381 pKa = 11.84GTRR384 pKa = 11.84GGARR388 pKa = 11.84RR389 pKa = 11.84RR390 pKa = 11.84APSARR395 pKa = 11.84RR396 pKa = 11.84RR397 pKa = 11.84GASAPRR403 pKa = 11.84RR404 pKa = 11.84PGSPRR409 pKa = 11.84RR410 pKa = 11.84PGPWPPRR417 pKa = 11.84RR418 pKa = 11.84RR419 pKa = 11.84WWRR422 pKa = 11.84RR423 pKa = 11.84RR424 pKa = 11.84ASRR427 pKa = 11.84ARR429 pKa = 11.84ARR431 pKa = 11.84RR432 pKa = 11.84RR433 pKa = 11.84DD434 pKa = 3.36GTRR437 pKa = 11.84AARR440 pKa = 11.84GARR443 pKa = 11.84AWPRR447 pKa = 11.84RR448 pKa = 11.84RR449 pKa = 11.84ACARR453 pKa = 11.84RR454 pKa = 11.84SARR457 pKa = 11.84RR458 pKa = 11.84RR459 pKa = 11.84RR460 pKa = 11.84GRR462 pKa = 11.84GAGAARR468 pKa = 11.84RR469 pKa = 11.84GSRR472 pKa = 11.84ARR474 pKa = 11.84ARR476 pKa = 11.84PRR478 pKa = 11.84RR479 pKa = 11.84ARR481 pKa = 11.84RR482 pKa = 11.84ARR484 pKa = 11.84ARR486 pKa = 11.84RR487 pKa = 11.84RR488 pKa = 11.84RR489 pKa = 11.84APRR492 pKa = 11.84ARR494 pKa = 11.84RR495 pKa = 11.84RR496 pKa = 11.84AGRR499 pKa = 11.84RR500 pKa = 11.84RR501 pKa = 11.84RR502 pKa = 11.84ASARR506 pKa = 11.84RR507 pKa = 11.84TGPRR511 pKa = 11.84GAPRR515 pKa = 11.84RR516 pKa = 11.84PGTRR520 pKa = 11.84AARR523 pKa = 11.84RR524 pKa = 11.84RR525 pKa = 11.84PGRR528 pKa = 11.84PRR530 pKa = 11.84AXASAGTALTTTSFRR545 pKa = 11.84PAAPASSPRR554 pKa = 11.84NSTAPAKK561 pKa = 10.33ARR563 pKa = 11.84SRR565 pKa = 11.84VEE567 pKa = 3.83SSSTPRR573 pKa = 11.84AFSAEE578 pKa = 4.09TSGGPVQLPAQAAEE592 pKa = 4.23SGVQRR597 pKa = 11.84RR598 pKa = 11.84LASAASRR605 pKa = 11.84NEE607 pKa = 3.85SPPRR611 pKa = 11.84SVVXLXGARR620 pKa = 11.84ARR622 pKa = 11.84ARR624 pKa = 11.84RR625 pKa = 11.84APRR628 pKa = 11.84RR629 pKa = 11.84AAARR633 pKa = 11.84RR634 pKa = 11.84ARR636 pKa = 11.84ARR638 pKa = 11.84ARR640 pKa = 11.84RR641 pKa = 11.84PGLAPGASTRR651 pKa = 11.84RR652 pKa = 11.84RR653 pKa = 11.84GAAGPRR659 pKa = 11.84RR660 pKa = 11.84AAGRR664 pKa = 11.84KK665 pKa = 9.13AGAAPALLACSRR677 pKa = 11.84ASSGAA682 pKa = 3.27

Molecular weight: 71.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

1

79

37865

41

2772

479.3

51.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.0 ± 0.484

1.997 ± 0.143

5.145 ± 0.172

4.838 ± 0.208

3.193 ± 0.157

7.558 ± 0.3

2.469 ± 0.098

3.251 ± 0.175

1.957 ± 0.134

8.818 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.108

2.113 ± 0.118

7.656 ± 0.416

2.673 ± 0.124

9.867 ± 0.537

7.099 ± 0.256

4.944 ± 0.148

6.626 ± 0.231

1.062 ± 0.067

2.496 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski