Gordonia phage Hedwig

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hedwigvirus; Gordonia virus Hedwig

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C9EHW3|A0A1C9EHW3_9CAUD Uncharacterized protein OS=Gordonia phage Hedwig OX=1887648 GN=49 PE=4 SV=1
MM1 pKa = 7.81MYY3 pKa = 10.3PPDD6 pKa = 3.83HH7 pKa = 6.88VGPDD11 pKa = 3.6EE12 pKa = 5.96DD13 pKa = 5.63DD14 pKa = 3.36GTMSDD19 pKa = 3.38NVADD23 pKa = 4.7LRR25 pKa = 11.84DD26 pKa = 3.53AVIGRR31 pKa = 11.84RR32 pKa = 11.84IVSAEE37 pKa = 3.75QADD40 pKa = 4.27FRR42 pKa = 11.84AADD45 pKa = 4.47FVASEE50 pKa = 4.21HH51 pKa = 4.88TAYY54 pKa = 9.72WSSFDD59 pKa = 3.98GNGLVLTLDD68 pKa = 3.67DD69 pKa = 4.0GRR71 pKa = 11.84RR72 pKa = 11.84VALVDD77 pKa = 3.53TSSCCAYY84 pKa = 9.86TDD86 pKa = 3.7LQEE89 pKa = 5.09FFLDD93 pKa = 3.78PDD95 pKa = 4.18LVDD98 pKa = 3.54HH99 pKa = 7.36AITGVATTDD108 pKa = 4.75GYY110 pKa = 6.7TTWHH114 pKa = 6.94IYY116 pKa = 11.07ADD118 pKa = 3.92LGDD121 pKa = 3.57IMRR124 pKa = 11.84LKK126 pKa = 10.83VGWSAGNPFYY136 pKa = 10.89YY137 pKa = 10.41GYY139 pKa = 10.91GFDD142 pKa = 4.0IGVSTTIEE150 pKa = 4.05GSLALPEE157 pKa = 4.32IEE159 pKa = 5.3GPSHH163 pKa = 7.46DD164 pKa = 3.78

Molecular weight: 17.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C9EHR0|A0A1C9EHR0_9CAUD Lysin B OS=Gordonia phage Hedwig OX=1887648 GN=28 PE=4 SV=1
MM1 pKa = 7.4HH2 pKa = 7.74KK3 pKa = 10.43DD4 pKa = 2.84IEE6 pKa = 4.45MEE8 pKa = 4.29SKK10 pKa = 10.87VIEE13 pKa = 3.83NWITHH18 pKa = 6.72LYY20 pKa = 11.01AEE22 pKa = 4.96GKK24 pKa = 9.64SNRR27 pKa = 11.84TVKK30 pKa = 10.42DD31 pKa = 3.07RR32 pKa = 11.84RR33 pKa = 11.84IVLRR37 pKa = 11.84RR38 pKa = 11.84FEE40 pKa = 4.58RR41 pKa = 11.84DD42 pKa = 2.39IGTPILAANTGQIAAWLGRR61 pKa = 11.84DD62 pKa = 3.35DD63 pKa = 4.62LAPVTRR69 pKa = 11.84SVYY72 pKa = 10.54HH73 pKa = 6.59SILAAFFRR81 pKa = 11.84WAIAVGLRR89 pKa = 11.84DD90 pKa = 4.92DD91 pKa = 5.1NPVSPIKK98 pKa = 10.11AAKK101 pKa = 9.59RR102 pKa = 11.84PRR104 pKa = 11.84RR105 pKa = 11.84QPRR108 pKa = 11.84PVTPEE113 pKa = 3.38QYY115 pKa = 10.36RR116 pKa = 11.84RR117 pKa = 11.84LLDD120 pKa = 3.92GAAGDD125 pKa = 4.21HH126 pKa = 7.31PITAMLLLAGLQGLRR141 pKa = 11.84VHH143 pKa = 7.11EE144 pKa = 4.1IARR147 pKa = 11.84FHH149 pKa = 6.78SRR151 pKa = 11.84HH152 pKa = 6.03LDD154 pKa = 2.99IEE156 pKa = 4.25ARR158 pKa = 11.84TLEE161 pKa = 4.17VTGKK165 pKa = 10.73GGTSYY170 pKa = 11.24VLPASQPILDD180 pKa = 4.45HH181 pKa = 6.45ARR183 pKa = 11.84NMPQTGFWFPSQRR196 pKa = 11.84AKK198 pKa = 10.86HH199 pKa = 5.51LGGRR203 pKa = 11.84TVSEE207 pKa = 4.67RR208 pKa = 11.84IHH210 pKa = 7.67LHH212 pKa = 5.49MIRR215 pKa = 11.84HH216 pKa = 5.78RR217 pKa = 11.84VPGTPHH223 pKa = 7.29CLRR226 pKa = 11.84HH227 pKa = 5.95HH228 pKa = 7.24FGTQLVSNGADD239 pKa = 2.95LRR241 pKa = 11.84VAQEE245 pKa = 4.07LLRR248 pKa = 11.84HH249 pKa = 5.97SSLQTTAIYY258 pKa = 10.64VATSDD263 pKa = 3.5DD264 pKa = 3.86RR265 pKa = 11.84KK266 pKa = 10.58RR267 pKa = 11.84EE268 pKa = 4.32AIDD271 pKa = 3.35RR272 pKa = 11.84LVAA275 pKa = 4.14

Molecular weight: 31.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

14207

46

1670

203.0

22.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.346 ± 0.526

0.824 ± 0.148

6.659 ± 0.333

5.744 ± 0.332

2.816 ± 0.212

8.651 ± 0.386

1.922 ± 0.164

4.533 ± 0.279

2.872 ± 0.157

7.883 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.161 ± 0.135

2.597 ± 0.142

6.011 ± 0.402

3.576 ± 0.187

7.475 ± 0.413

5.793 ± 0.206

6.687 ± 0.288

7.37 ± 0.245

2.041 ± 0.145

2.041 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski