Amino acid dipepetide frequency for Myotis lucifugus (Little brown bat)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.74AlaAla: 6.74 ± 0.048
1.458AlaCys: 1.458 ± 0.015
2.935AlaAsp: 2.935 ± 0.019
4.702AlaGlu: 4.702 ± 0.026
2.63AlaPhe: 2.63 ± 0.019
4.845AlaGly: 4.845 ± 0.032
1.612AlaHis: 1.612 ± 0.013
2.797AlaIle: 2.797 ± 0.018
3.401AlaLys: 3.401 ± 0.025
7.274AlaLeu: 7.274 ± 0.039
1.518AlaMet: 1.518 ± 0.014
2.004AlaAsn: 2.004 ± 0.014
4.345AlaPro: 4.345 ± 0.035
3.329AlaGln: 3.329 ± 0.027
3.707AlaArg: 3.707 ± 0.026
5.716AlaSer: 5.716 ± 0.03
3.503AlaThr: 3.503 ± 0.02
4.705AlaVal: 4.705 ± 0.026
0.827AlaTrp: 0.827 ± 0.01
1.539AlaTyr: 1.539 ± 0.015
0.002AlaXaa: 0.002 ± 0.001
Cys
1.353CysAla: 1.353 ± 0.014
0.718CysCys: 0.718 ± 0.02
1.018CysAsp: 1.018 ± 0.015
1.266CysGlu: 1.266 ± 0.015
0.883CysPhe: 0.883 ± 0.01
1.667CysGly: 1.667 ± 0.019
0.69CysHis: 0.69 ± 0.01
0.964CysIle: 0.964 ± 0.011
1.128CysLys: 1.128 ± 0.012
2.233CysLeu: 2.233 ± 0.018
0.416CysMet: 0.416 ± 0.007
0.795CysAsn: 0.795 ± 0.011
1.468CysPro: 1.468 ± 0.019
1.103CysGln: 1.103 ± 0.016
1.356CysArg: 1.356 ± 0.014
2.053CysSer: 2.053 ± 0.018
1.138CysThr: 1.138 ± 0.014
1.333CysVal: 1.333 ± 0.012
0.316CysTrp: 0.316 ± 0.006
0.617CysTyr: 0.617 ± 0.01
0.001CysXaa: 0.001 ± 0.0
Asp
2.827AspAla: 2.827 ± 0.02
1.05AspCys: 1.05 ± 0.013
2.527AspAsp: 2.527 ± 0.021
3.319AspGlu: 3.319 ± 0.022
2.109AspPhe: 2.109 ± 0.018
3.256AspGly: 3.256 ± 0.025
1.159AspHis: 1.159 ± 0.011
2.6AspIle: 2.6 ± 0.019
2.485AspLys: 2.485 ± 0.018
4.989AspLeu: 4.989 ± 0.024
1.115AspMet: 1.115 ± 0.011
1.645AspAsn: 1.645 ± 0.017
2.967AspPro: 2.967 ± 0.022
1.843AspGln: 1.843 ± 0.014
2.462AspArg: 2.462 ± 0.02
4.106AspSer: 4.106 ± 0.026
2.498AspThr: 2.498 ± 0.016
3.011AspVal: 3.011 ± 0.027
0.633AspTrp: 0.633 ± 0.01
1.453AspTyr: 1.453 ± 0.015
0.001AspXaa: 0.001 ± 0.0
Glu
5.246GluAla: 5.246 ± 0.034
1.304GluCys: 1.304 ± 0.019
4.389GluAsp: 4.389 ± 0.027
7.81GluGlu: 7.81 ± 0.05
2.047GluPhe: 2.047 ± 0.013
4.238GluGly: 4.238 ± 0.026
1.5GluHis: 1.5 ± 0.012
3.08GluIle: 3.08 ± 0.024
5.173GluLys: 5.173 ± 0.036
6.385GluLeu: 6.385 ± 0.038
1.638GluMet: 1.638 ± 0.015
3.054GluAsn: 3.054 ± 0.021
3.301GluPro: 3.301 ± 0.029
3.114GluGln: 3.114 ± 0.024
3.877GluArg: 3.877 ± 0.03
4.274GluSer: 4.274 ± 0.028
3.266GluThr: 3.266 ± 0.019
4.161GluVal: 4.161 ± 0.026
0.734GluTrp: 0.734 ± 0.009
1.549GluTyr: 1.549 ± 0.017
0.002GluXaa: 0.002 ± 0.0
Phe
1.983PheAla: 1.983 ± 0.017
0.995PheCys: 0.995 ± 0.011
1.659PheAsp: 1.659 ± 0.014
1.98PheGlu: 1.98 ± 0.016
1.702PhePhe: 1.702 ± 0.016
2.235PheGly: 2.235 ± 0.018
1.066PheHis: 1.066 ± 0.011
1.872PheIle: 1.872 ± 0.017
1.793PheLys: 1.793 ± 0.015
4.313PheLeu: 4.313 ± 0.027
0.762PheMet: 0.762 ± 0.008
1.325PheAsn: 1.325 ± 0.013
2.098PhePro: 2.098 ± 0.017
1.845PheGln: 1.845 ± 0.014
2.008PheArg: 2.008 ± 0.018
3.5PheSer: 3.5 ± 0.021
2.034PheThr: 2.034 ± 0.017
2.177PheVal: 2.177 ± 0.017
0.508PheTrp: 0.508 ± 0.007
1.25PheTyr: 1.25 ± 0.012
0.001PheXaa: 0.001 ± 0.0
Gly
4.643GlyAla: 4.643 ± 0.035
1.328GlyCys: 1.328 ± 0.014
3.124GlyAsp: 3.124 ± 0.021
4.077GlyGlu: 4.077 ± 0.029
2.47GlyPhe: 2.47 ± 0.02
5.127GlyGly: 5.127 ± 0.05
1.757GlyHis: 1.757 ± 0.017
2.716GlyIle: 2.716 ± 0.023
3.739GlyLys: 3.739 ± 0.027
6.092GlyLeu: 6.092 ± 0.035
1.274GlyMet: 1.274 ± 0.012
2.36GlyAsn: 2.36 ± 0.018
4.481GlyPro: 4.481 ± 0.055
2.806GlyGln: 2.806 ± 0.022
3.847GlyArg: 3.847 ± 0.025
5.846GlySer: 5.846 ± 0.031
3.614GlyThr: 3.614 ± 0.023
3.638GlyVal: 3.638 ± 0.023
0.825GlyTrp: 0.825 ± 0.011
1.733GlyTyr: 1.733 ± 0.018
0.002GlyXaa: 0.002 ± 0.0
His
1.334HisAla: 1.334 ± 0.014
0.741HisCys: 0.741 ± 0.01
0.866HisAsp: 0.866 ± 0.01
1.26HisGlu: 1.26 ± 0.012
1.157HisPhe: 1.157 ± 0.012
1.568HisGly: 1.568 ± 0.014
0.876HisHis: 0.876 ± 0.012
1.263HisIle: 1.263 ± 0.012
1.234HisLys: 1.234 ± 0.013
3.003HisLeu: 3.003 ± 0.02
0.584HisMet: 0.584 ± 0.009
0.862HisAsn: 0.862 ± 0.01
1.725HisPro: 1.725 ± 0.015
1.305HisGln: 1.305 ± 0.015
1.658HisArg: 1.658 ± 0.014
2.276HisSer: 2.276 ± 0.018
1.449HisThr: 1.449 ± 0.014
1.524HisVal: 1.524 ± 0.013
0.368HisTrp: 0.368 ± 0.006
0.824HisTyr: 0.824 ± 0.009
0.001HisXaa: 0.001 ± 0.0
Ile
2.549IleAla: 2.549 ± 0.019
1.101IleCys: 1.101 ± 0.012
1.968IleAsp: 1.968 ± 0.017
2.497IleGlu: 2.497 ± 0.02
1.93IlePhe: 1.93 ± 0.019
2.182IleGly: 2.182 ± 0.02
1.315IleHis: 1.315 ± 0.012
2.362IleIle: 2.362 ± 0.024
2.56IleLys: 2.56 ± 0.022
4.643IleLeu: 4.643 ± 0.029
0.983IleMet: 0.983 ± 0.009
1.78IleAsn: 1.78 ± 0.017
2.589IlePro: 2.589 ± 0.017
2.265IleGln: 2.265 ± 0.015
2.368IleArg: 2.368 ± 0.018
3.689IleSer: 3.689 ± 0.023
2.525IleThr: 2.525 ± 0.021
2.476IleVal: 2.476 ± 0.021
0.549IleTrp: 0.549 ± 0.009
1.42IleTyr: 1.42 ± 0.011
0.001IleXaa: 0.001 ± 0.0
Lys
3.998LysAla: 3.998 ± 0.028
1.09LysCys: 1.09 ± 0.013
3.095LysAsp: 3.095 ± 0.025
4.981LysGlu: 4.981 ± 0.037
1.73LysPhe: 1.73 ± 0.016
3.312LysGly: 3.312 ± 0.032
1.392LysHis: 1.392 ± 0.013
2.735LysIle: 2.735 ± 0.019
4.705LysLys: 4.705 ± 0.04
5.088LysLeu: 5.088 ± 0.026
1.439LysMet: 1.439 ± 0.015
2.373LysAsn: 2.373 ± 0.019
3.043LysPro: 3.043 ± 0.028
2.558LysGln: 2.558 ± 0.022
3.273LysArg: 3.273 ± 0.021
3.801LysSer: 3.801 ± 0.029
3.046LysThr: 3.046 ± 0.022
3.455LysVal: 3.455 ± 0.032
0.597LysTrp: 0.597 ± 0.008
1.58LysTyr: 1.58 ± 0.015
0.001LysXaa: 0.001 ± 0.0
Leu
6.911LeuAla: 6.911 ± 0.037
2.29LeuCys: 2.29 ± 0.018
4.749LeuAsp: 4.749 ± 0.026
7.118LeuGlu: 7.118 ± 0.04
3.539LeuPhe: 3.539 ± 0.024
6.068LeuGly: 6.068 ± 0.033
2.752LeuHis: 2.752 ± 0.016
3.885LeuIle: 3.885 ± 0.028
5.793LeuLys: 5.793 ± 0.035
11.052LeuLeu: 11.052 ± 0.065
1.957LeuMet: 1.957 ± 0.015
3.477LeuAsn: 3.477 ± 0.023
6.151LeuPro: 6.151 ± 0.035
5.839LeuGln: 5.839 ± 0.038
6.071LeuArg: 6.071 ± 0.035
8.205LeuSer: 8.205 ± 0.033
5.15LeuThr: 5.15 ± 0.023
5.726LeuVal: 5.726 ± 0.033
1.195LeuTrp: 1.195 ± 0.013
2.582LeuTyr: 2.582 ± 0.018
0.003LeuXaa: 0.003 ± 0.0
Met
1.901MetAla: 1.901 ± 0.016
0.405MetCys: 0.405 ± 0.007
1.22MetAsp: 1.22 ± 0.012
1.806MetGlu: 1.806 ± 0.014
0.729MetPhe: 0.729 ± 0.009
1.278MetGly: 1.278 ± 0.013
0.451MetHis: 0.451 ± 0.007
0.816MetIle: 0.816 ± 0.009
1.419MetLys: 1.419 ± 0.014
1.991MetLeu: 1.991 ± 0.018
0.535MetMet: 0.535 ± 0.009
0.88MetAsn: 0.88 ± 0.009
1.074MetPro: 1.074 ± 0.016
0.902MetGln: 0.902 ± 0.01
1.0MetArg: 1.0 ± 0.01
1.545MetSer: 1.545 ± 0.015
1.089MetThr: 1.089 ± 0.012
1.361MetVal: 1.361 ± 0.014
0.24MetTrp: 0.24 ± 0.005
0.617MetTyr: 0.617 ± 0.01
0.001MetXaa: 0.001 ± 0.0
Asn
2.037AsnAla: 2.037 ± 0.015
0.853AsnCys: 0.853 ± 0.01
1.48AsnAsp: 1.48 ± 0.014
2.157AsnGlu: 2.157 ± 0.019
1.487AsnPhe: 1.487 ± 0.013
2.357AsnGly: 2.357 ± 0.018
0.926AsnHis: 0.926 ± 0.01
2.081AsnIle: 2.081 ± 0.017
2.181AsnLys: 2.181 ± 0.017
3.712AsnLeu: 3.712 ± 0.022
0.884AsnMet: 0.884 ± 0.009
1.504AsnAsn: 1.504 ± 0.016
2.189AsnPro: 2.189 ± 0.017
1.632AsnGln: 1.632 ± 0.015
1.815AsnArg: 1.815 ± 0.013
2.998AsnSer: 2.998 ± 0.022
1.94AsnThr: 1.94 ± 0.014
2.188AsnVal: 2.188 ± 0.015
0.455AsnTrp: 0.455 ± 0.007
1.123AsnTyr: 1.123 ± 0.012
0.001AsnXaa: 0.001 ± 0.0
Pro
4.923ProAla: 4.923 ± 0.034
1.207ProCys: 1.207 ± 0.017
2.763ProAsp: 2.763 ± 0.022
4.487ProGlu: 4.487 ± 0.031
1.949ProPhe: 1.949 ± 0.015
5.589ProGly: 5.589 ± 0.076
1.467ProHis: 1.467 ± 0.014
1.88ProIle: 1.88 ± 0.018
2.851ProLys: 2.851 ± 0.033
5.401ProLeu: 5.401 ± 0.03
1.082ProMet: 1.082 ± 0.012
1.808ProAsn: 1.808 ± 0.013
6.111ProPro: 6.111 ± 0.058
2.88ProGln: 2.88 ± 0.024
3.538ProArg: 3.538 ± 0.027
5.731ProSer: 5.731 ± 0.035
3.089ProThr: 3.089 ± 0.024
3.858ProVal: 3.858 ± 0.026
0.738ProTrp: 0.738 ± 0.01
1.423ProTyr: 1.423 ± 0.015
0.002ProXaa: 0.002 ± 0.0
Gln
3.598GlnAla: 3.598 ± 0.025
0.95GlnCys: 0.95 ± 0.014
2.35GlnAsp: 2.35 ± 0.019
3.93GlnGlu: 3.93 ± 0.03
1.364GlnPhe: 1.364 ± 0.012
2.957GlnGly: 2.957 ± 0.023
1.294GlnHis: 1.294 ± 0.014
1.951GlnIle: 1.951 ± 0.014
2.912GlnLys: 2.912 ± 0.024
4.789GlnLeu: 4.789 ± 0.034
1.107GlnMet: 1.107 ± 0.012
1.84GlnAsn: 1.84 ± 0.019
2.827GlnPro: 2.827 ± 0.028
3.01GlnGln: 3.01 ± 0.039
2.97GlnArg: 2.97 ± 0.023
3.053GlnSer: 3.053 ± 0.024
2.275GlnThr: 2.275 ± 0.017
2.923GlnVal: 2.923 ± 0.019
0.552GlnTrp: 0.552 ± 0.008
1.131GlnTyr: 1.131 ± 0.012
0.001GlnXaa: 0.001 ± 0.0
Arg
3.96ArgAla: 3.96 ± 0.027
1.277ArgCys: 1.277 ± 0.017
2.793ArgAsp: 2.793 ± 0.021
3.992ArgGlu: 3.992 ± 0.029
1.925ArgPhe: 1.925 ± 0.016
3.754ArgGly: 3.754 ± 0.031
1.552ArgHis: 1.552 ± 0.015
2.341ArgIle: 2.341 ± 0.018
3.716ArgLys: 3.716 ± 0.023
5.449ArgLeu: 5.449 ± 0.029
1.143ArgMet: 1.143 ± 0.012
2.117ArgAsn: 2.117 ± 0.014
3.331ArgPro: 3.331 ± 0.025
2.624ArgGln: 2.624 ± 0.02
4.356ArgArg: 4.356 ± 0.04
4.186ArgSer: 4.186 ± 0.035
2.784ArgThr: 2.784 ± 0.02
3.192ArgVal: 3.192 ± 0.022
0.731ArgTrp: 0.731 ± 0.009
1.494ArgTyr: 1.494 ± 0.013
0.002ArgXaa: 0.002 ± 0.0
Ser
5.279SerAla: 5.279 ± 0.027
1.922SerCys: 1.922 ± 0.021
3.828SerAsp: 3.828 ± 0.027
5.048SerGlu: 5.048 ± 0.031
3.085SerPhe: 3.085 ± 0.019
5.627SerGly: 5.627 ± 0.029
2.138SerHis: 2.138 ± 0.019
3.174SerIle: 3.174 ± 0.021
3.995SerLys: 3.995 ± 0.027
8.39SerLeu: 8.39 ± 0.037
1.538SerMet: 1.538 ± 0.012
2.635SerAsn: 2.635 ± 0.019
5.998SerPro: 5.998 ± 0.041
3.849SerGln: 3.849 ± 0.027
4.516SerArg: 4.516 ± 0.035
8.974SerSer: 8.974 ± 0.066
4.297SerThr: 4.297 ± 0.026
4.907SerVal: 4.907 ± 0.027
1.114SerTrp: 1.114 ± 0.013
2.098SerTyr: 2.098 ± 0.017
0.002SerXaa: 0.002 ± 0.0
Thr
3.696ThrAla: 3.696 ± 0.023
1.373ThrCys: 1.373 ± 0.017
2.424ThrAsp: 2.424 ± 0.017
3.449ThrGlu: 3.449 ± 0.021
2.149ThrPhe: 2.149 ± 0.016
3.486ThrGly: 3.486 ± 0.027
1.282ThrHis: 1.282 ± 0.014
2.388ThrIle: 2.388 ± 0.023
2.62ThrLys: 2.62 ± 0.02
5.328ThrLeu: 5.328 ± 0.027
1.074ThrMet: 1.074 ± 0.01
1.702ThrAsn: 1.702 ± 0.016
3.494ThrPro: 3.494 ± 0.027
2.279ThrGln: 2.279 ± 0.018
2.44ThrArg: 2.44 ± 0.018
4.486ThrSer: 4.486 ± 0.027
2.854ThrThr: 2.854 ± 0.027
3.867ThrVal: 3.867 ± 0.028
0.709ThrTrp: 0.709 ± 0.009
1.456ThrTyr: 1.456 ± 0.015
0.002ThrXaa: 0.002 ± 0.0
Val
4.299ValAla: 4.299 ± 0.024
1.478ValCys: 1.478 ± 0.012
2.91ValAsp: 2.91 ± 0.021
3.803ValGlu: 3.803 ± 0.027
2.455ValPhe: 2.455 ± 0.018
3.463ValGly: 3.463 ± 0.023
1.581ValHis: 1.581 ± 0.014
2.913ValIle: 2.913 ± 0.023
3.348ValLys: 3.348 ± 0.027
6.393ValLeu: 6.393 ± 0.034
1.308ValMet: 1.308 ± 0.013
2.196ValAsn: 2.196 ± 0.019
3.737ValPro: 3.737 ± 0.03
2.797ValGln: 2.797 ± 0.017
3.076ValArg: 3.076 ± 0.022
4.881ValSer: 4.881 ± 0.026
3.833ValThr: 3.833 ± 0.035
3.996ValVal: 3.996 ± 0.03
0.712ValTrp: 0.712 ± 0.009
1.659ValTyr: 1.659 ± 0.015
0.001ValXaa: 0.001 ± 0.0
Trp
0.867TrpAla: 0.867 ± 0.009
0.255TrpCys: 0.255 ± 0.005
0.668TrpAsp: 0.668 ± 0.011
0.836TrpGlu: 0.836 ± 0.011
0.455TrpPhe: 0.455 ± 0.007
0.784TrpGly: 0.784 ± 0.011
0.319TrpHis: 0.319 ± 0.006
0.565TrpIle: 0.565 ± 0.007
0.803TrpLys: 0.803 ± 0.01
1.245TrpLeu: 1.245 ± 0.013
0.313TrpMet: 0.313 ± 0.005
0.535TrpAsn: 0.535 ± 0.009
0.534TrpPro: 0.534 ± 0.007
0.562TrpGln: 0.562 ± 0.008
0.755TrpArg: 0.755 ± 0.009
0.89TrpSer: 0.89 ± 0.01
0.685TrpThr: 0.685 ± 0.009
0.759TrpVal: 0.759 ± 0.009
0.198TrpTrp: 0.198 ± 0.005
0.353TrpTyr: 0.353 ± 0.006
0.001TrpXaa: 0.001 ± 0.0
Tyr
1.443TyrAla: 1.443 ± 0.013
0.719TyrCys: 0.719 ± 0.01
1.287TyrAsp: 1.287 ± 0.014
1.624TyrGlu: 1.624 ± 0.017
1.309TyrPhe: 1.309 ± 0.013
1.703TyrGly: 1.703 ± 0.016
0.751TyrHis: 0.751 ± 0.01
1.368TyrIle: 1.368 ± 0.012
1.435TyrLys: 1.435 ± 0.018
2.739TyrLeu: 2.739 ± 0.019
0.589TyrMet: 0.589 ± 0.009
1.107TyrAsn: 1.107 ± 0.012
1.31TyrPro: 1.31 ± 0.014
1.241TyrGln: 1.241 ± 0.012
1.624TyrArg: 1.624 ± 0.015
2.202TyrSer: 2.202 ± 0.021
1.489TyrThr: 1.489 ± 0.017
1.584TyrVal: 1.584 ± 0.015
0.377TyrTrp: 0.377 ± 0.008
0.998TyrTyr: 0.998 ± 0.012
0.001TyrXaa: 0.001 ± 0.0
Xaa
0.002XaaAla: 0.002 ± 0.0
0.001XaaCys: 0.001 ± 0.0
0.001XaaAsp: 0.001 ± 0.0
0.001XaaGlu: 0.001 ± 0.0
0.001XaaPhe: 0.001 ± 0.0
0.002XaaGly: 0.002 ± 0.001
0.001XaaHis: 0.001 ± 0.0
0.001XaaIle: 0.001 ± 0.0
0.001XaaLys: 0.001 ± 0.0
0.002XaaLeu: 0.002 ± 0.0
0.001XaaMet: 0.001 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.003XaaPro: 0.003 ± 0.001
0.002XaaGln: 0.002 ± 0.0
0.002XaaArg: 0.002 ± 0.0
0.003XaaSer: 0.003 ± 0.001
0.001XaaThr: 0.001 ± 0.0
0.001XaaVal: 0.001 ± 0.0
0.001XaaTrp: 0.001 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.007XaaXaa: 0.007 ± 0.002
Statistics based on 20651 proteins (10579164 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski