Hyphomicrobium sulfonivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Hyphomicrobium

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A109BPF2|A0A109BPF2_HYPSL Transcriptional regulator GntR family domain OS=Hyphomicrobium sulfonivorans OX=121290 GN=APY04_0079 PE=3 SV=1
MM1 pKa = 6.95VLAVAASLASALGVHH16 pKa = 6.94ADD18 pKa = 3.86ALASSITNRR27 pKa = 11.84DD28 pKa = 2.99DD29 pKa = 3.1TAYY32 pKa = 9.77KK33 pKa = 10.23ISIVEE38 pKa = 3.99EE39 pKa = 3.93NAVAEE44 pKa = 4.48HH45 pKa = 6.34NLAPSATVTGICAGGCVLHH64 pKa = 6.84LNRR67 pKa = 11.84DD68 pKa = 3.83PDD70 pKa = 3.66HH71 pKa = 7.6DD72 pKa = 4.53GYY74 pKa = 11.56NLQGTDD80 pKa = 3.22VVSIEE85 pKa = 5.13DD86 pKa = 3.33GLLYY90 pKa = 10.75YY91 pKa = 9.94DD92 pKa = 5.17APPAAAAPAVGDD104 pKa = 3.7DD105 pKa = 3.69ADD107 pKa = 4.21RR108 pKa = 11.84PEE110 pKa = 4.21VPRR113 pKa = 11.84NEE115 pKa = 4.03

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A109BM08|A0A109BM08_HYPSL Uncharacterized protein OS=Hyphomicrobium sulfonivorans OX=121290 GN=APY04_0505 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.26RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.73GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MQTVGGVRR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3495

0

3495

1023349

37

2002

292.8

31.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.72 ± 0.067

0.861 ± 0.017

5.695 ± 0.027

5.681 ± 0.036

3.604 ± 0.026

8.366 ± 0.043

2.02 ± 0.022

5.171 ± 0.032

3.545 ± 0.033

9.607 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.02

2.883 ± 0.026

5.209 ± 0.034

3.292 ± 0.026

7.018 ± 0.039

5.698 ± 0.032

5.341 ± 0.033

7.318 ± 0.041

1.291 ± 0.017

2.294 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski