Hubei mosquito virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHU0|A0A1L3KHU0_9VIRU Uncharacterized protein OS=Hubei mosquito virus 3 OX=1922927 PE=4 SV=1
MM1 pKa = 6.83QRR3 pKa = 11.84DD4 pKa = 3.53MSGRR8 pKa = 11.84RR9 pKa = 11.84TPEE12 pKa = 4.22GSCSEE17 pKa = 4.05PSRR20 pKa = 11.84ARR22 pKa = 11.84FSVPPCEE29 pKa = 4.81PPDD32 pKa = 3.79GFCPVVQGSTLSKK45 pKa = 9.75TRR47 pKa = 11.84RR48 pKa = 11.84QTLRR52 pKa = 11.84GWITCRR58 pKa = 11.84PCGGIDD64 pKa = 3.0TSMILVLHH72 pKa = 5.56VQHH75 pKa = 6.69SKK77 pKa = 11.08LVFCSLSCEE86 pKa = 3.91MRR88 pKa = 11.84LCLSQGTATWNVYY101 pKa = 7.8VVYY104 pKa = 10.52LPAQASDD111 pKa = 3.63FSRR114 pKa = 11.84TIQGAEE120 pKa = 3.59FPMRR124 pKa = 11.84IEE126 pKa = 4.16AVPFIDD132 pKa = 3.55VTEE135 pKa = 4.36LLGSPIVVLPRR146 pKa = 11.84HH147 pKa = 6.4RR148 pKa = 11.84GQDD151 pKa = 3.37SASVEE156 pKa = 4.23MFSPNHH162 pKa = 6.2LGAEE166 pKa = 4.43CAEE169 pKa = 4.33LPGVEE174 pKa = 4.63CFPGGLHH181 pKa = 6.2EE182 pKa = 4.93AQFCGIIRR190 pKa = 11.84PPLLLRR196 pKa = 11.84VGGVCQSIVTAHH208 pKa = 6.52NEE210 pKa = 3.77FLHH213 pKa = 7.04AGLSDD218 pKa = 4.31DD219 pKa = 5.26RR220 pKa = 11.84GIPQAKK226 pKa = 8.98KK227 pKa = 9.96RR228 pKa = 11.84KK229 pKa = 9.03RR230 pKa = 11.84DD231 pKa = 3.53QGPHH235 pKa = 6.57KK236 pKa = 10.43GPPALPKK243 pKa = 10.53

Molecular weight: 26.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHU0|A0A1L3KHU0_9VIRU Uncharacterized protein OS=Hubei mosquito virus 3 OX=1922927 PE=4 SV=1
MM1 pKa = 7.25VRR3 pKa = 11.84RR4 pKa = 11.84AYY6 pKa = 9.93DD7 pKa = 3.56LKK9 pKa = 11.09RR10 pKa = 11.84PLPYY14 pKa = 10.44SQVEE18 pKa = 3.95AAKK21 pKa = 10.15PCAHH25 pKa = 6.22QVKK28 pKa = 8.01TWKK31 pKa = 10.45EE32 pKa = 3.78FTNFLLDD39 pKa = 3.89LVAHH43 pKa = 6.72GKK45 pKa = 9.33SALVRR50 pKa = 11.84RR51 pKa = 11.84PGSTPFTRR59 pKa = 11.84SFTSFIVSRR68 pKa = 11.84CAWSRR73 pKa = 11.84GRR75 pKa = 11.84GKK77 pKa = 9.2WASIAFSLGQLKK89 pKa = 10.13RR90 pKa = 11.84CFPALPDD97 pKa = 3.43EE98 pKa = 4.55MRR100 pKa = 11.84RR101 pKa = 11.84EE102 pKa = 4.19ALDD105 pKa = 3.16KK106 pKa = 10.94HH107 pKa = 6.13QEE109 pKa = 4.18LLGSAAEE116 pKa = 4.29PFVEE120 pKa = 5.15FPIVDD125 pKa = 5.01RR126 pKa = 11.84IIQQYY131 pKa = 9.58FPVNWARR138 pKa = 11.84KK139 pKa = 7.23TSVKK143 pKa = 10.32VYY145 pKa = 10.62NAIPNTACIEE155 pKa = 4.37GGSCRR160 pKa = 11.84DD161 pKa = 3.69YY162 pKa = 11.21LASTGPKK169 pKa = 8.65PARR172 pKa = 11.84GCTPIPSSGQILAKK186 pKa = 9.95RR187 pKa = 11.84RR188 pKa = 11.84YY189 pKa = 9.2EE190 pKa = 4.31SIFDD194 pKa = 3.75KK195 pKa = 11.19LYY197 pKa = 8.86MQRR200 pKa = 11.84LQPLPTLKK208 pKa = 10.64AVAVPDD214 pKa = 3.71AGKK217 pKa = 10.25FRR219 pKa = 11.84IITIDD224 pKa = 3.58SVSAKK229 pKa = 9.98CLQPVQRR236 pKa = 11.84ALLSHH241 pKa = 7.09LSRR244 pKa = 11.84FPEE247 pKa = 4.41FEE249 pKa = 4.05FCTSTFQGEE258 pKa = 4.59LPPKK262 pKa = 7.45WGKK265 pKa = 7.05MAKK268 pKa = 9.91GEE270 pKa = 4.14KK271 pKa = 9.82LLSVDD276 pKa = 3.99YY277 pKa = 11.53SNATDD282 pKa = 3.95GVDD285 pKa = 3.19GSFMSEE291 pKa = 3.69LMDD294 pKa = 5.59RR295 pKa = 11.84IIDD298 pKa = 3.53RR299 pKa = 11.84SGSFEE304 pKa = 4.22LLALRR309 pKa = 11.84EE310 pKa = 4.02QAVQEE315 pKa = 4.3TTLGRR320 pKa = 11.84RR321 pKa = 11.84ISNDD325 pKa = 2.83LCSFWQRR332 pKa = 11.84RR333 pKa = 11.84GTLMGSLVSFPLLCLWNASIIRR355 pKa = 11.84EE356 pKa = 3.99AGMKK360 pKa = 10.2KK361 pKa = 10.54FIVCGDD367 pKa = 3.69DD368 pKa = 3.11ALAYY372 pKa = 7.74ATNTQKK378 pKa = 10.57KK379 pKa = 8.18RR380 pKa = 11.84WADD383 pKa = 3.45YY384 pKa = 9.88SAKK387 pKa = 10.34LGLVQSPGKK396 pKa = 8.36TFHH399 pKa = 6.22SRR401 pKa = 11.84KK402 pKa = 9.86FGTFCSKK409 pKa = 10.59VVGTEE414 pKa = 3.57HH415 pKa = 7.03LNAGRR420 pKa = 11.84ILAPMSGEE428 pKa = 4.01NYY430 pKa = 10.12NRR432 pKa = 11.84TPKK435 pKa = 10.06QFRR438 pKa = 11.84HH439 pKa = 5.26IYY441 pKa = 9.83KK442 pKa = 9.85RR443 pKa = 11.84YY444 pKa = 8.5CFYY447 pKa = 10.59PHH449 pKa = 6.93GEE451 pKa = 3.94FRR453 pKa = 11.84SLYY456 pKa = 9.11GPRR459 pKa = 11.84EE460 pKa = 3.74IGGLGGEE467 pKa = 4.36VDD469 pKa = 5.37DD470 pKa = 5.15IDD472 pKa = 4.16VPSRR476 pKa = 11.84RR477 pKa = 11.84ALRR480 pKa = 11.84KK481 pKa = 9.37AQAHH485 pKa = 6.83LARR488 pKa = 11.84QGTKK492 pKa = 9.98YY493 pKa = 10.1QLGMLDD499 pKa = 3.64MQDD502 pKa = 3.55EE503 pKa = 4.44NHH505 pKa = 6.5AGIYY509 pKa = 5.33TTAWSACDD517 pKa = 3.54PTPQRR522 pKa = 11.84LTPRR526 pKa = 11.84LRR528 pKa = 11.84KK529 pKa = 9.97GGSLDD534 pKa = 3.58YY535 pKa = 9.46RR536 pKa = 11.84TKK538 pKa = 9.95PIRR541 pKa = 11.84WFTRR545 pKa = 11.84WDD547 pKa = 3.39RR548 pKa = 11.84KK549 pKa = 9.53SRR551 pKa = 11.84SRR553 pKa = 11.84RR554 pKa = 11.84FF555 pKa = 3.14

Molecular weight: 62.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

798

243

555

399.0

44.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.268 ± 0.746

3.258 ± 0.832

4.386 ± 0.338

4.637 ± 0.353

4.887 ± 0.382

7.769 ± 0.636

2.256 ± 0.513

4.511 ± 0.008

5.263 ± 1.179

8.897 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.168

1.88 ± 0.319

7.018 ± 1.212

4.386 ± 0.273

8.897 ± 0.534

7.895 ± 0.166

5.138 ± 0.303

5.388 ± 0.999

1.629 ± 0.399

2.506 ± 0.833
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski