Gordonia phage Guacamole

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142KBN8|A0A142KBN8_9CAUD Minor tail protein OS=Gordonia phage Guacamole OX=1821553 GN=30 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.85GSGGEE26 pKa = 4.14SGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.86NPWYY45 pKa = 10.31DD46 pKa = 3.26PTYY49 pKa = 11.17NYY51 pKa = 11.17GLDD54 pKa = 4.0PNGHH58 pKa = 5.86TEE60 pKa = 4.02MTDD63 pKa = 2.45PWKK66 pKa = 10.7RR67 pKa = 11.84HH68 pKa = 3.99TEE70 pKa = 4.05FPEE73 pKa = 4.17VCSMGFGGPSIGLPTDD89 pKa = 3.96PPPPPVEE96 pKa = 4.3PEE98 pKa = 3.82PTPDD102 pKa = 4.39LEE104 pKa = 4.41EE105 pKa = 4.43PTDD108 pKa = 3.7GG109 pKa = 4.88

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142KBT6|A0A142KBT6_9CAUD HNH endonuclease OS=Gordonia phage Guacamole OX=1821553 GN=78 PE=4 SV=1
MM1 pKa = 7.57ARR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84PNPEE9 pKa = 3.24QRR11 pKa = 11.84GLGHH15 pKa = 6.69AHH17 pKa = 6.24KK18 pKa = 10.55QQVKK22 pKa = 9.27HH23 pKa = 5.94LKK25 pKa = 7.95TRR27 pKa = 11.84HH28 pKa = 5.47VDD30 pKa = 3.6GTPCWWCGKK39 pKa = 9.42PMYY42 pKa = 10.3LDD44 pKa = 3.19RR45 pKa = 11.84TRR47 pKa = 11.84NPDD50 pKa = 3.69YY51 pKa = 11.07NPTSPDD57 pKa = 3.55PASGSLAGDD66 pKa = 3.23HH67 pKa = 6.01TLARR71 pKa = 11.84THH73 pKa = 6.66GGTLADD79 pKa = 3.84RR80 pKa = 11.84LLHH83 pKa = 5.94GRR85 pKa = 11.84CNKK88 pKa = 9.38QRR90 pKa = 11.84GDD92 pKa = 3.25GRR94 pKa = 11.84HH95 pKa = 5.79DD96 pKa = 3.32HH97 pKa = 6.33TRR99 pKa = 11.84PALTGKK105 pKa = 8.91HH106 pKa = 4.65QPAPTTHH113 pKa = 7.03LAINCWPP120 pKa = 3.68

Molecular weight: 13.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16205

47

1840

207.8

22.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.651 ± 0.445

0.821 ± 0.141

6.955 ± 0.326

5.467 ± 0.341

2.826 ± 0.135

8.374 ± 0.407

2.073 ± 0.169

4.554 ± 0.162

3.314 ± 0.25

7.874 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.265 ± 0.191

2.832 ± 0.137

5.924 ± 0.237

3.382 ± 0.147

7.461 ± 0.358

5.227 ± 0.211

6.714 ± 0.244

7.893 ± 0.237

2.129 ± 0.117

2.265 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski