Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / CCUG 14189 / LMG 1036 / NCIMB 9965 / AN6)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Morganellaceae; Xenorhabdus; Xenorhabdus nematophila

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4439 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3VH49|D3VH49_XENNA Putative Sarcosine oxidase OS=Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / CCUG 14189 / LMG 1036 / NCIMB 9965 / AN6) OX=406817 GN=XNC1_0246 PE=4 SV=1
MM1 pKa = 7.68ILPTLTLKK9 pKa = 11.01GYY11 pKa = 6.92TVCSLHH17 pKa = 7.18KK18 pKa = 10.36PDD20 pKa = 4.41VNPMKK25 pKa = 10.56YY26 pKa = 8.68DD27 pKa = 3.41TSEE30 pKa = 3.95LCDD33 pKa = 3.85IYY35 pKa = 11.2QEE37 pKa = 4.9DD38 pKa = 4.12INVVEE43 pKa = 4.46PLFSNFGGRR52 pKa = 11.84TSFGGQIITVKK63 pKa = 10.47CFEE66 pKa = 5.19DD67 pKa = 3.7NGLLYY72 pKa = 10.72DD73 pKa = 4.02LLEE76 pKa = 4.67EE77 pKa = 4.49NGHH80 pKa = 5.62GRR82 pKa = 11.84ILLVDD87 pKa = 3.85GGGSVRR93 pKa = 11.84KK94 pKa = 9.76ALVDD98 pKa = 3.5AEE100 pKa = 4.31LAQLAANNEE109 pKa = 3.91WEE111 pKa = 4.69GIVVYY116 pKa = 10.2GAVRR120 pKa = 11.84QVDD123 pKa = 4.15YY124 pKa = 11.19LSEE127 pKa = 4.52LDD129 pKa = 3.87IGIQAMAAIPAGSNSEE145 pKa = 4.17GVGEE149 pKa = 3.96SDD151 pKa = 3.17IRR153 pKa = 11.84VNFGGVTFFSGDD165 pKa = 3.38YY166 pKa = 10.53LYY168 pKa = 11.22ADD170 pKa = 3.32NTGIVLSDD178 pKa = 3.59VPLEE182 pKa = 4.08LDD184 pKa = 3.8EE185 pKa = 6.94DD186 pKa = 4.13NDD188 pKa = 4.47DD189 pKa = 3.67VV190 pKa = 4.68

Molecular weight: 20.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3VG89|D3VG89_XENNA Cytosine/purines uracil thiamine allantoin permease family protein (Modular protein) OS=Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / CCUG 14189 / LMG 1036 / NCIMB 9965 / AN6) OX=406817 GN=XNC1_0091 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4439

0

4439

1253860

20

5994

282.5

31.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.156 ± 0.045

1.137 ± 0.015

5.152 ± 0.03

6.005 ± 0.036

3.927 ± 0.027

6.734 ± 0.051

2.484 ± 0.022

6.751 ± 0.036

5.187 ± 0.044

10.575 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.022

4.408 ± 0.035

4.262 ± 0.03

4.723 ± 0.04

5.354 ± 0.037

6.375 ± 0.027

5.341 ± 0.03

6.298 ± 0.032

1.371 ± 0.016

3.205 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski