Mycobacterium phage Sbash

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Chenonavirus; Mycobacterium virus Sbash

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7RVR2|A0A0A7RVR2_9CAUD Uncharacterized protein OS=Mycobacterium phage Sbash OX=1567475 GN=58 PE=4 SV=1
MM1 pKa = 7.7INPTDD6 pKa = 3.73AEE8 pKa = 4.33DD9 pKa = 3.91YY10 pKa = 10.76EE11 pKa = 4.45FAFYY15 pKa = 10.3FSVKK19 pKa = 9.2GVKK22 pKa = 8.39VTGPGDD28 pKa = 3.98PDD30 pKa = 2.73TWYY33 pKa = 10.24PNIQYY38 pKa = 10.75GLRR41 pKa = 11.84GVGEE45 pKa = 4.46APPPLILQAYY55 pKa = 8.48LMNVGLNSDD64 pKa = 3.7PQSVRR69 pKa = 11.84FAILYY74 pKa = 6.72TEE76 pKa = 4.41PVTWTAVSPEE86 pKa = 4.04EE87 pKa = 4.22VAALPGSVEE96 pKa = 4.55DD97 pKa = 3.61YY98 pKa = 11.17SFAIFGDD105 pKa = 3.97VPADD109 pKa = 3.54EE110 pKa = 4.99NGNAFRR116 pKa = 11.84LGLVGSGEE124 pKa = 4.2YY125 pKa = 10.43GRR127 pKa = 11.84EE128 pKa = 3.87VSLLTLPIYY137 pKa = 10.79LDD139 pKa = 3.51TQGQEE144 pKa = 4.07GALHH148 pKa = 7.14DD149 pKa = 4.25VALCYY154 pKa = 10.34APRR157 pKa = 11.84VNWSVWEE164 pKa = 3.94VDD166 pKa = 3.14AA167 pKa = 6.61

Molecular weight: 18.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7RVM2|A0A0A7RVM2_9CAUD Uncharacterized protein OS=Mycobacterium phage Sbash OX=1567475 GN=23 PE=4 SV=1
MM1 pKa = 7.52TEE3 pKa = 4.16ARR5 pKa = 11.84WALLVSVLTLVVNTIAVVQNRR26 pKa = 11.84KK27 pKa = 8.58PRR29 pKa = 11.84PGKK32 pKa = 8.71RR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.41RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84HH40 pKa = 4.45RR41 pKa = 11.84KK42 pKa = 8.82RR43 pKa = 3.23

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

18090

29

1221

203.3

22.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.653 ± 0.405

1.139 ± 0.157

6.633 ± 0.164

5.948 ± 0.292

2.648 ± 0.177

8.894 ± 0.562

2.117 ± 0.24

4.229 ± 0.14

3.19 ± 0.211

8.292 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.084 ± 0.112

2.88 ± 0.183

6.49 ± 0.233

3.615 ± 0.176

7.358 ± 0.359

4.981 ± 0.209

6.368 ± 0.201

6.971 ± 0.225

2.095 ± 0.142

2.416 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski