Botrytis cinerea mitovirus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus; unclassified Mitovirus

Average proteome isoelectric point is 8.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S4GA85|A0A0S4GA85_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 4 OX=1629667 GN=RdRp PE=4 SV=1
MM1 pKa = 7.42SNKK4 pKa = 9.58IINIIIIRR12 pKa = 11.84IIRR15 pKa = 11.84LCFNYY20 pKa = 10.38NEE22 pKa = 4.58STWEE26 pKa = 3.63INEE29 pKa = 3.97FLNIFNNMRR38 pKa = 11.84KK39 pKa = 9.71KK40 pKa = 10.69SGLKK44 pKa = 8.67YY45 pKa = 8.58TIKK48 pKa = 10.49YY49 pKa = 7.87YY50 pKa = 10.74KK51 pKa = 10.03AVKK54 pKa = 9.98LHH56 pKa = 4.82ITRR59 pKa = 11.84YY60 pKa = 9.05ICGSPLLSNKK70 pKa = 10.39DD71 pKa = 3.51GVSVDD76 pKa = 3.21ASGWPTKK83 pKa = 10.53FIFLKK88 pKa = 10.71KK89 pKa = 10.42FIKK92 pKa = 9.27TRR94 pKa = 11.84QGLRR98 pKa = 11.84ILLTLLSFTRR108 pKa = 11.84TVVPTKK114 pKa = 10.4QEE116 pKa = 3.87EE117 pKa = 4.39LKK119 pKa = 10.65IKK121 pKa = 10.05PDD123 pKa = 3.26YY124 pKa = 10.33STIDD128 pKa = 3.11KK129 pKa = 10.84LYY131 pKa = 8.7TGKK134 pKa = 10.51NYY136 pKa = 8.37TIPAWFIKK144 pKa = 10.26SWINKK149 pKa = 8.85HH150 pKa = 4.87NLKK153 pKa = 10.74AKK155 pKa = 9.84IPTYY159 pKa = 9.91TKK161 pKa = 8.87EE162 pKa = 3.73DD163 pKa = 3.86HH164 pKa = 5.92YY165 pKa = 11.88VSMKK169 pKa = 10.46GSPNGPATYY178 pKa = 10.43SSLWSILSLSYY189 pKa = 10.28PQLQNISTMLGDD201 pKa = 3.77YY202 pKa = 10.32RR203 pKa = 11.84DD204 pKa = 3.95EE205 pKa = 4.05FFKK208 pKa = 10.66FYY210 pKa = 8.96KK211 pKa = 9.24TAWEE215 pKa = 4.19NNFGNDD221 pKa = 3.96SSIKK225 pKa = 8.29SSKK228 pKa = 6.63WTGYY232 pKa = 7.77TGKK235 pKa = 10.61LSIVKK240 pKa = 10.24DD241 pKa = 3.69PEE243 pKa = 3.88LKK245 pKa = 10.27RR246 pKa = 11.84RR247 pKa = 11.84VIAMVDD253 pKa = 3.16YY254 pKa = 10.83HH255 pKa = 6.82SQFTLKK261 pKa = 10.17PIHH264 pKa = 6.83EE265 pKa = 4.35MLLNKK270 pKa = 9.89LRR272 pKa = 11.84TLKK275 pKa = 10.44CDD277 pKa = 3.05RR278 pKa = 11.84TFTQDD283 pKa = 4.13PKK285 pKa = 10.98HH286 pKa = 5.65SWYY289 pKa = 10.96VNNEE293 pKa = 3.79KK294 pKa = 10.47FFSLDD299 pKa = 3.02LSAATDD305 pKa = 3.96RR306 pKa = 11.84FPLQLQKK313 pKa = 11.35KK314 pKa = 8.25LLSYY318 pKa = 10.27IYY320 pKa = 9.76EE321 pKa = 4.29NKK323 pKa = 10.31DD324 pKa = 3.3FCDD327 pKa = 3.16AWADD331 pKa = 3.81LLTSRR336 pKa = 11.84VYY338 pKa = 10.45IDD340 pKa = 4.66SDD342 pKa = 3.94GLQHH346 pKa = 7.08KK347 pKa = 9.93YY348 pKa = 10.81NVGQPMGAYY357 pKa = 9.89SSWAAFTITHH367 pKa = 6.31HH368 pKa = 7.05LVVAWAAHH376 pKa = 5.7LCGEE380 pKa = 4.62YY381 pKa = 10.72NFSQYY386 pKa = 10.7IILGDD391 pKa = 4.59DD392 pKa = 3.31IVIKK396 pKa = 10.53NNKK399 pKa = 7.97VANKK403 pKa = 9.9YY404 pKa = 7.31ITLMTRR410 pKa = 11.84LGVEE414 pKa = 3.87ISLHH418 pKa = 5.06KK419 pKa = 9.43THH421 pKa = 6.28VSKK424 pKa = 10.14DD425 pKa = 3.14TYY427 pKa = 10.9EE428 pKa = 3.97FAKK431 pKa = 10.31RR432 pKa = 11.84WIKK435 pKa = 10.86DD436 pKa = 3.54GIEE439 pKa = 3.85VSGIPLKK446 pKa = 11.08GILNQWKK453 pKa = 9.43FPGVVYY459 pKa = 7.53TTLEE463 pKa = 4.01SFFDD467 pKa = 3.93KK468 pKa = 11.24NPIQPKK474 pKa = 10.21SLIDD478 pKa = 4.57LICGLYY484 pKa = 10.57KK485 pKa = 10.53NLPLGKK491 pKa = 9.52RR492 pKa = 11.84RR493 pKa = 11.84MSYY496 pKa = 9.03NQIYY500 pKa = 9.96KK501 pKa = 10.66LLYY504 pKa = 9.8DD505 pKa = 3.43YY506 pKa = 10.54HH507 pKa = 7.85HH508 pKa = 7.3AMRR511 pKa = 11.84YY512 pKa = 8.49SLNKK516 pKa = 8.22ITYY519 pKa = 8.87DD520 pKa = 3.26EE521 pKa = 4.26LRR523 pKa = 11.84AYY525 pKa = 10.44LCSKK529 pKa = 10.24CKK531 pKa = 9.95EE532 pKa = 4.09DD533 pKa = 5.1SFVLPYY539 pKa = 10.74EE540 pKa = 4.66SISLHH545 pKa = 5.51FMKK548 pKa = 10.85LLLSGGMVSEE558 pKa = 4.35AEE560 pKa = 4.12KK561 pKa = 10.51VSRR564 pKa = 11.84FILSEE569 pKa = 3.8YY570 pKa = 10.12TKK572 pKa = 10.34IEE574 pKa = 4.08NKK576 pKa = 10.28FKK578 pKa = 11.0DD579 pKa = 3.62SYY581 pKa = 11.65SDD583 pKa = 4.09LNILSGYY590 pKa = 9.03PLLNGYY596 pKa = 8.75YY597 pKa = 10.24NHH599 pKa = 6.59LQSMQGKK606 pKa = 8.93ILDD609 pKa = 3.83WEE611 pKa = 4.23KK612 pKa = 11.35DD613 pKa = 3.63PNVTLVDD620 pKa = 3.62SALSLRR626 pKa = 11.84IEE628 pKa = 4.26KK629 pKa = 9.96FDD631 pKa = 5.36KK632 pKa = 10.28ISSMNRR638 pKa = 11.84DD639 pKa = 2.71KK640 pKa = 11.04SVRR643 pKa = 11.84VSTLSGLWKK652 pKa = 9.65TSMKK656 pKa = 10.2RR657 pKa = 11.84LWVEE661 pKa = 4.06RR662 pKa = 11.84IEE664 pKa = 5.88DD665 pKa = 3.75DD666 pKa = 3.74FEE668 pKa = 5.34YY669 pKa = 8.29MTFFSRR675 pKa = 11.84INKK678 pKa = 8.66DD679 pKa = 3.01QHH681 pKa = 8.09DD682 pKa = 4.3SMLPVHH688 pKa = 6.14GWEE691 pKa = 4.4GVLDD695 pKa = 3.97NNIQFTLNQLKK706 pKa = 9.83PLISGTIVKK715 pKa = 9.71VEE717 pKa = 4.01KK718 pKa = 10.92NSWEE722 pKa = 3.96NLDD725 pKa = 3.43WGDD728 pKa = 4.03FKK730 pKa = 11.81VV731 pKa = 3.4

Molecular weight:
85.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0S4GA85|A0A0S4GA85_9VIRU RNA dependent RNA polymerase OS=Botrytis cinerea mitovirus 4 OX=1629667 GN=RdRp PE=4 SV=1
MM1 pKa = 7.42SNKK4 pKa = 9.58IINIIIIRR12 pKa = 11.84IIRR15 pKa = 11.84LCFNYY20 pKa = 10.38NEE22 pKa = 4.58STWEE26 pKa = 3.63INEE29 pKa = 3.97FLNIFNNMRR38 pKa = 11.84KK39 pKa = 9.71KK40 pKa = 10.69SGLKK44 pKa = 8.67YY45 pKa = 8.58TIKK48 pKa = 10.49YY49 pKa = 7.87YY50 pKa = 10.74KK51 pKa = 10.03AVKK54 pKa = 9.98LHH56 pKa = 4.82ITRR59 pKa = 11.84YY60 pKa = 9.05ICGSPLLSNKK70 pKa = 10.39DD71 pKa = 3.51GVSVDD76 pKa = 3.21ASGWPTKK83 pKa = 10.53FIFLKK88 pKa = 10.71KK89 pKa = 10.42FIKK92 pKa = 9.27TRR94 pKa = 11.84QGLRR98 pKa = 11.84ILLTLLSFTRR108 pKa = 11.84TVVPTKK114 pKa = 10.4QEE116 pKa = 3.87EE117 pKa = 4.39LKK119 pKa = 10.65IKK121 pKa = 10.05PDD123 pKa = 3.26YY124 pKa = 10.33STIDD128 pKa = 3.11KK129 pKa = 10.84LYY131 pKa = 8.7TGKK134 pKa = 10.51NYY136 pKa = 8.37TIPAWFIKK144 pKa = 10.26SWINKK149 pKa = 8.85HH150 pKa = 4.87NLKK153 pKa = 10.74AKK155 pKa = 9.84IPTYY159 pKa = 9.91TKK161 pKa = 8.87EE162 pKa = 3.73DD163 pKa = 3.86HH164 pKa = 5.92YY165 pKa = 11.88VSMKK169 pKa = 10.46GSPNGPATYY178 pKa = 10.43SSLWSILSLSYY189 pKa = 10.28PQLQNISTMLGDD201 pKa = 3.77YY202 pKa = 10.32RR203 pKa = 11.84DD204 pKa = 3.95EE205 pKa = 4.05FFKK208 pKa = 10.66FYY210 pKa = 8.96KK211 pKa = 9.24TAWEE215 pKa = 4.19NNFGNDD221 pKa = 3.96SSIKK225 pKa = 8.29SSKK228 pKa = 6.63WTGYY232 pKa = 7.77TGKK235 pKa = 10.61LSIVKK240 pKa = 10.24DD241 pKa = 3.69PEE243 pKa = 3.88LKK245 pKa = 10.27RR246 pKa = 11.84RR247 pKa = 11.84VIAMVDD253 pKa = 3.16YY254 pKa = 10.83HH255 pKa = 6.82SQFTLKK261 pKa = 10.17PIHH264 pKa = 6.83EE265 pKa = 4.35MLLNKK270 pKa = 9.89LRR272 pKa = 11.84TLKK275 pKa = 10.44CDD277 pKa = 3.05RR278 pKa = 11.84TFTQDD283 pKa = 4.13PKK285 pKa = 10.98HH286 pKa = 5.65SWYY289 pKa = 10.96VNNEE293 pKa = 3.79KK294 pKa = 10.47FFSLDD299 pKa = 3.02LSAATDD305 pKa = 3.96RR306 pKa = 11.84FPLQLQKK313 pKa = 11.35KK314 pKa = 8.25LLSYY318 pKa = 10.27IYY320 pKa = 9.76EE321 pKa = 4.29NKK323 pKa = 10.31DD324 pKa = 3.3FCDD327 pKa = 3.16AWADD331 pKa = 3.81LLTSRR336 pKa = 11.84VYY338 pKa = 10.45IDD340 pKa = 4.66SDD342 pKa = 3.94GLQHH346 pKa = 7.08KK347 pKa = 9.93YY348 pKa = 10.81NVGQPMGAYY357 pKa = 9.89SSWAAFTITHH367 pKa = 6.31HH368 pKa = 7.05LVVAWAAHH376 pKa = 5.7LCGEE380 pKa = 4.62YY381 pKa = 10.72NFSQYY386 pKa = 10.7IILGDD391 pKa = 4.59DD392 pKa = 3.31IVIKK396 pKa = 10.53NNKK399 pKa = 7.97VANKK403 pKa = 9.9YY404 pKa = 7.31ITLMTRR410 pKa = 11.84LGVEE414 pKa = 3.87ISLHH418 pKa = 5.06KK419 pKa = 9.43THH421 pKa = 6.28VSKK424 pKa = 10.14DD425 pKa = 3.14TYY427 pKa = 10.9EE428 pKa = 3.97FAKK431 pKa = 10.31RR432 pKa = 11.84WIKK435 pKa = 10.86DD436 pKa = 3.54GIEE439 pKa = 3.85VSGIPLKK446 pKa = 11.08GILNQWKK453 pKa = 9.43FPGVVYY459 pKa = 7.53TTLEE463 pKa = 4.01SFFDD467 pKa = 3.93KK468 pKa = 11.24NPIQPKK474 pKa = 10.21SLIDD478 pKa = 4.57LICGLYY484 pKa = 10.57KK485 pKa = 10.53NLPLGKK491 pKa = 9.52RR492 pKa = 11.84RR493 pKa = 11.84MSYY496 pKa = 9.03NQIYY500 pKa = 9.96KK501 pKa = 10.66LLYY504 pKa = 9.8DD505 pKa = 3.43YY506 pKa = 10.54HH507 pKa = 7.85HH508 pKa = 7.3AMRR511 pKa = 11.84YY512 pKa = 8.49SLNKK516 pKa = 8.22ITYY519 pKa = 8.87DD520 pKa = 3.26EE521 pKa = 4.26LRR523 pKa = 11.84AYY525 pKa = 10.44LCSKK529 pKa = 10.24CKK531 pKa = 9.95EE532 pKa = 4.09DD533 pKa = 5.1SFVLPYY539 pKa = 10.74EE540 pKa = 4.66SISLHH545 pKa = 5.51FMKK548 pKa = 10.85LLLSGGMVSEE558 pKa = 4.35AEE560 pKa = 4.12KK561 pKa = 10.51VSRR564 pKa = 11.84FILSEE569 pKa = 3.8YY570 pKa = 10.12TKK572 pKa = 10.34IEE574 pKa = 4.08NKK576 pKa = 10.28FKK578 pKa = 11.0DD579 pKa = 3.62SYY581 pKa = 11.65SDD583 pKa = 4.09LNILSGYY590 pKa = 9.03PLLNGYY596 pKa = 8.75YY597 pKa = 10.24NHH599 pKa = 6.59LQSMQGKK606 pKa = 8.93ILDD609 pKa = 3.83WEE611 pKa = 4.23KK612 pKa = 11.35DD613 pKa = 3.63PNVTLVDD620 pKa = 3.62SALSLRR626 pKa = 11.84IEE628 pKa = 4.26KK629 pKa = 9.96FDD631 pKa = 5.36KK632 pKa = 10.28ISSMNRR638 pKa = 11.84DD639 pKa = 2.71KK640 pKa = 11.04SVRR643 pKa = 11.84VSTLSGLWKK652 pKa = 9.65TSMKK656 pKa = 10.2RR657 pKa = 11.84LWVEE661 pKa = 4.06RR662 pKa = 11.84IEE664 pKa = 5.88DD665 pKa = 3.75DD666 pKa = 3.74FEE668 pKa = 5.34YY669 pKa = 8.29MTFFSRR675 pKa = 11.84INKK678 pKa = 8.66DD679 pKa = 3.01QHH681 pKa = 8.09DD682 pKa = 4.3SMLPVHH688 pKa = 6.14GWEE691 pKa = 4.4GVLDD695 pKa = 3.97NNIQFTLNQLKK706 pKa = 9.83PLISGTIVKK715 pKa = 9.71VEE717 pKa = 4.01KK718 pKa = 10.92NSWEE722 pKa = 3.96NLDD725 pKa = 3.43WGDD728 pKa = 4.03FKK730 pKa = 11.81VV731 pKa = 3.4

Molecular weight:
85.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

731

731

731

731.0

85.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.146 ± 0.0

1.094 ± 0.0

5.746 ± 0.0

4.378 ± 0.0

4.651 ± 0.0

4.651 ± 0.0

2.462 ± 0.0

7.934 ± 0.0

9.986 ± 0.0

10.807 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.0

5.882 ± 0.0

3.283 ± 0.0

2.599 ± 0.0

3.694 ± 0.0

8.755 ± 0.0

5.609 ± 0.0

4.651 ± 0.0

2.599 ± 0.0

5.746 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski