Sida leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2WCL6|Q2WCL6_9GEMI Transcriptional activator protein OS=Sida leaf curl virus OX=337823 GN=AC2 PE=3 SV=1
MM1 pKa = 7.43APSKK5 pKa = 10.57RR6 pKa = 11.84FNIYY10 pKa = 9.69CKK12 pKa = 10.48NYY14 pKa = 8.63FLTYY18 pKa = 9.03PKK20 pKa = 10.53CSLTKK25 pKa = 10.68EE26 pKa = 4.19EE27 pKa = 4.64TLSQIQNLNTPTNKK41 pKa = 10.14KK42 pKa = 9.03YY43 pKa = 10.74IKK45 pKa = 10.03VCRR48 pKa = 11.84EE49 pKa = 3.48LHH51 pKa = 6.31EE52 pKa = 4.82NGEE55 pKa = 4.21PHH57 pKa = 6.01IHH59 pKa = 6.22VLIQFEE65 pKa = 4.73GKK67 pKa = 9.95YY68 pKa = 9.77KK69 pKa = 10.46CQNQRR74 pKa = 11.84FFDD77 pKa = 3.9LVSPNRR83 pKa = 11.84SAHH86 pKa = 5.06FHH88 pKa = 6.56PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.8KK106 pKa = 11.28DD107 pKa = 3.26GDD109 pKa = 3.83TIEE112 pKa = 4.07WGEE115 pKa = 3.76FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.05AYY134 pKa = 10.67AAALNTGSKK143 pKa = 10.36PEE145 pKa = 3.84ALRR148 pKa = 11.84VLRR151 pKa = 11.84EE152 pKa = 4.28LAPKK156 pKa = 10.32DD157 pKa = 3.57YY158 pKa = 11.19VLQFHH163 pKa = 6.76NLNANLDD170 pKa = 4.31RR171 pKa = 11.84IFTPPMEE178 pKa = 4.56VYY180 pKa = 10.6VSPFSVSSFDD190 pKa = 3.59RR191 pKa = 11.84VPEE194 pKa = 3.9EE195 pKa = 3.98LEE197 pKa = 3.58EE198 pKa = 4.19WACEE202 pKa = 4.05NVVAAAARR210 pKa = 11.84PLRR213 pKa = 11.84PLSLVVEE220 pKa = 4.84GDD222 pKa = 3.31SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 4.79DD262 pKa = 4.33VDD264 pKa = 3.73PHH266 pKa = 6.08FLKK269 pKa = 10.67HH270 pKa = 5.9FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.07KK312 pKa = 10.49EE313 pKa = 3.93YY314 pKa = 10.94LDD316 pKa = 3.87EE317 pKa = 4.82EE318 pKa = 4.69KK319 pKa = 11.14NSALKK324 pKa = 9.03TWALKK329 pKa = 10.03NASFVTLTEE338 pKa = 3.89PLYY341 pKa = 11.27SGAHH345 pKa = 4.59QNRR348 pKa = 11.84ASEE351 pKa = 4.36GEE353 pKa = 4.25EE354 pKa = 3.6EE355 pKa = 4.41VQSQEE360 pKa = 3.92EE361 pKa = 4.49GGPP364 pKa = 3.47

Molecular weight: 41.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2WCL8|Q2WCL8_9GEMI Capsid protein OS=Sida leaf curl virus OX=337823 GN=AV1 PE=3 SV=1
MM1 pKa = 7.57SKK3 pKa = 10.33RR4 pKa = 11.84PASMAYY10 pKa = 9.83SSPISSARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.12SPRR27 pKa = 11.84PSVAAALTAPGIRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84WTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.85PRR54 pKa = 11.84FYY56 pKa = 11.08RR57 pKa = 11.84MYY59 pKa = 9.54RR60 pKa = 11.84TPDD63 pKa = 3.19VPKK66 pKa = 10.64GCEE69 pKa = 4.39GPCKK73 pKa = 10.37VQSFDD78 pKa = 3.49AKK80 pKa = 11.12DD81 pKa = 4.42DD82 pKa = 3.56IGHH85 pKa = 5.87MGKK88 pKa = 10.22VICLSDD94 pKa = 3.33VTRR97 pKa = 11.84GIGLTHH103 pKa = 6.67RR104 pKa = 11.84VGKK107 pKa = 9.78RR108 pKa = 11.84FCVKK112 pKa = 10.05SLYY115 pKa = 10.31FVGKK119 pKa = 9.14IWMDD123 pKa = 3.34EE124 pKa = 4.02NIKK127 pKa = 10.41VKK129 pKa = 10.64NHH131 pKa = 5.76TNTVMFWIVRR141 pKa = 11.84DD142 pKa = 3.82RR143 pKa = 11.84RR144 pKa = 11.84PTGTPLDD151 pKa = 3.85FQQVFNVYY159 pKa = 10.6DD160 pKa = 3.98NEE162 pKa = 4.32PATATVKK169 pKa = 10.43NDD171 pKa = 2.65KK172 pKa = 10.2RR173 pKa = 11.84DD174 pKa = 3.33RR175 pKa = 11.84FQVLRR180 pKa = 11.84RR181 pKa = 11.84FHH183 pKa = 6.07ATVTGGQYY191 pKa = 10.21AAKK194 pKa = 9.32EE195 pKa = 3.85SAIIRR200 pKa = 11.84KK201 pKa = 9.02FYY203 pKa = 10.59RR204 pKa = 11.84VNNYY208 pKa = 7.79VVYY211 pKa = 10.32NHH213 pKa = 6.0QEE215 pKa = 3.43AGKK218 pKa = 10.41YY219 pKa = 8.39EE220 pKa = 4.09NHH222 pKa = 6.52TEE224 pKa = 3.99NALLLYY230 pKa = 7.29MACTHH235 pKa = 7.07ASNPVYY241 pKa = 10.04ATLKK245 pKa = 9.26CRR247 pKa = 11.84SYY249 pKa = 10.95FYY251 pKa = 10.51DD252 pKa = 3.2TVTNN256 pKa = 4.1

Molecular weight: 29.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1113

100

364

185.5

21.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.66 ± 0.733

2.336 ± 0.529

4.672 ± 0.235

5.211 ± 1.056

4.582 ± 0.23

5.211 ± 0.476

3.504 ± 0.535

4.313 ± 0.682

5.481 ± 0.752

7.098 ± 0.794

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.066 ± 0.46

5.75 ± 0.55

5.93 ± 0.625

4.942 ± 0.675

7.457 ± 1.214

8.266 ± 1.179

5.84 ± 1.121

6.02 ± 0.713

1.438 ± 0.151

4.223 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski