Bacillus salarius

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R9P520|A0A3R9P520_9BACI RNA methyltransferase OS=Bacillus salarius OX=284579 GN=D7Z54_13290 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 10.36QLLQKK7 pKa = 10.55EE8 pKa = 4.51SGVTLIEE15 pKa = 4.02LLATIVISSIVIGLVTSVLVSSLNFNDD42 pKa = 3.58KK43 pKa = 9.03TQSHH47 pKa = 6.36INLRR51 pKa = 11.84QEE53 pKa = 3.74ANIIITEE60 pKa = 4.31LRR62 pKa = 11.84QQHH65 pKa = 5.28QEE67 pKa = 3.91GEE69 pKa = 4.36YY70 pKa = 9.14TLCPEE75 pKa = 4.99DD76 pKa = 4.09VFSSDD81 pKa = 3.18RR82 pKa = 11.84FRR84 pKa = 11.84AVQRR88 pKa = 11.84DD89 pKa = 3.36IRR91 pKa = 11.84NDD93 pKa = 2.71EE94 pKa = 4.31HH95 pKa = 7.01MITSCNTVDD104 pKa = 3.33SQFPLEE110 pKa = 4.18VQFTLEE116 pKa = 4.24DD117 pKa = 3.93DD118 pKa = 4.08EE119 pKa = 6.22NNDD122 pKa = 3.38FTIDD126 pKa = 3.66TIIEE130 pKa = 4.09GEE132 pKa = 4.17RR133 pKa = 11.84QNGDD137 pKa = 3.57TNVSIDD143 pKa = 3.74PPGDD147 pKa = 3.51EE148 pKa = 4.44SDD150 pKa = 3.88SFPTYY155 pKa = 11.02VEE157 pKa = 4.3DD158 pKa = 4.06EE159 pKa = 4.28NVFVYY164 pKa = 10.31GSQFTFQGSDD174 pKa = 3.3VNGPGASMVIKK185 pKa = 10.65GPLDD189 pKa = 3.19MSEE192 pKa = 4.52FNGGSKK198 pKa = 9.77TNVSNIYY205 pKa = 10.12VDD207 pKa = 3.99GPIDD211 pKa = 3.77FSGGGQDD218 pKa = 3.0LGSYY222 pKa = 9.09EE223 pKa = 4.5EE224 pKa = 4.77PGEE227 pKa = 3.77IHH229 pKa = 7.16INGDD233 pKa = 3.18FDD235 pKa = 4.07TGGGSHH241 pKa = 7.28NIYY244 pKa = 9.85GDD246 pKa = 3.66VYY248 pKa = 10.9VEE250 pKa = 4.16EE251 pKa = 5.24DD252 pKa = 3.46FHH254 pKa = 9.34LEE256 pKa = 3.69GANIYY261 pKa = 10.84GDD263 pKa = 3.77VYY265 pKa = 11.63VNGDD269 pKa = 3.49VTLSDD274 pKa = 4.23YY275 pKa = 11.63YY276 pKa = 11.36SIAKK280 pKa = 9.06NASIHH285 pKa = 4.76YY286 pKa = 7.83TGSLPYY292 pKa = 8.86PDD294 pKa = 4.0HH295 pKa = 7.25FEE297 pKa = 4.71RR298 pKa = 11.84SDD300 pKa = 3.42FDD302 pKa = 4.25SLVKK306 pKa = 10.51QEE308 pKa = 4.52SVPNAEE314 pKa = 4.84IPDD317 pKa = 3.76QEE319 pKa = 4.53VPSSKK324 pKa = 10.61SEE326 pKa = 3.28NWYY329 pKa = 10.72AEE331 pKa = 3.69NGYY334 pKa = 6.69TQEE337 pKa = 4.01IQEE340 pKa = 5.15DD341 pKa = 3.94GMKK344 pKa = 9.82IYY346 pKa = 10.88DD347 pKa = 3.8SDD349 pKa = 4.25VVIEE353 pKa = 4.46DD354 pKa = 4.03NVNGSYY360 pKa = 10.86QDD362 pKa = 3.37TFTDD366 pKa = 3.51SVVVSEE372 pKa = 4.4GDD374 pKa = 3.24ITISGGNLSMTGVLYY389 pKa = 10.78APNGEE394 pKa = 4.12ITFEE398 pKa = 4.15GASFEE403 pKa = 4.35GTVIAKK409 pKa = 10.44DD410 pKa = 3.63GFNVDD415 pKa = 3.86SGGTDD420 pKa = 2.71ITFVGVEE427 pKa = 4.01EE428 pKa = 4.71YY429 pKa = 10.71INNRR433 pKa = 11.84DD434 pKa = 3.84DD435 pKa = 3.81YY436 pKa = 11.32PFF438 pKa = 5.07

Molecular weight: 48.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3R9RCE8|A0A3R9RCE8_9BACI 3-isopropylmalate dehydrogenase OS=Bacillus salarius OX=284579 GN=leuB PE=3 SV=1
MM1 pKa = 7.67GKK3 pKa = 7.99PTFTPNNRR11 pKa = 11.84KK12 pKa = 9.22RR13 pKa = 11.84KK14 pKa = 7.99KK15 pKa = 8.43VHH17 pKa = 5.59GFRR20 pKa = 11.84EE21 pKa = 4.09RR22 pKa = 11.84MSTKK26 pKa = 10.29NGRR29 pKa = 11.84QVLKK33 pKa = 10.21SRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.09GRR40 pKa = 11.84KK41 pKa = 9.06VISAA45 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6761

0

6761

1924827

24

2295

284.7

31.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.091 ± 0.034

0.675 ± 0.008

5.608 ± 0.029

8.26 ± 0.041

4.391 ± 0.024

6.932 ± 0.028

2.189 ± 0.015

7.207 ± 0.029

5.997 ± 0.029

9.242 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.966 ± 0.016

4.43 ± 0.02

3.651 ± 0.016

3.931 ± 0.023

4.063 ± 0.024

6.24 ± 0.024

5.636 ± 0.019

7.008 ± 0.024

1.087 ± 0.011

3.396 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski