Candidatus Accumulibacter sp. BA-91

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter; unclassified Candidatus Accumulibacter

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084Y6J2|A0A084Y6J2_9PROT 50S ribosomal protein L4 OS=Candidatus Accumulibacter sp. BA-91 OX=1454002 GN=rplD PE=3 SV=1
MM1 pKa = 7.57TIANLTLAQVLDD13 pKa = 3.8QLNSGRR19 pKa = 11.84SWSGASISYY28 pKa = 9.32SFPNTAPGLYY38 pKa = 9.92SQGEE42 pKa = 4.03AAAFRR47 pKa = 11.84AFDD50 pKa = 3.82ADD52 pKa = 3.54QRR54 pKa = 11.84SMMTLAMVTWDD65 pKa = 5.05DD66 pKa = 4.57LISPNFVQGTAGTTHH81 pKa = 7.43IEE83 pKa = 4.03FGYY86 pKa = 6.12TTSXIGFAHH95 pKa = 7.55AYY97 pKa = 8.33YY98 pKa = 10.17PEE100 pKa = 4.68IFGPRR105 pKa = 11.84YY106 pKa = 8.57AAPYY110 pKa = 8.72YY111 pKa = 10.46SSEE114 pKa = 4.02VMQADD119 pKa = 3.47WTGSDD124 pKa = 2.88GRR126 pKa = 11.84TYY128 pKa = 10.86SAQTPMLNDD137 pKa = 2.99VMAIQAIYY145 pKa = 9.65GASTSTRR152 pKa = 11.84LDD154 pKa = 3.11NTVYY158 pKa = 10.77GFGSNIVDD166 pKa = 3.79STRR169 pKa = 11.84AIYY172 pKa = 10.36DD173 pKa = 3.62FSLNAHH179 pKa = 7.61PILTLYY185 pKa = 10.61DD186 pKa = 3.54AGGLDD191 pKa = 3.97TLNLSGWSTPSRR203 pKa = 11.84IDD205 pKa = 3.02LHH207 pKa = 8.18AGAFSSGNDD216 pKa = 3.21MSNNIAIAYY225 pKa = 7.55NATIEE230 pKa = 4.05NAEE233 pKa = 4.18GGGGNDD239 pKa = 3.69VITGNDD245 pKa = 3.31TANLLRR251 pKa = 11.84GAAGNDD257 pKa = 3.36EE258 pKa = 5.1LYY260 pKa = 11.15GSAGDD265 pKa = 3.75DD266 pKa = 3.52TLIGGTGNDD275 pKa = 4.36DD276 pKa = 3.56LRR278 pKa = 11.84GGAGTDD284 pKa = 3.16TAVFEE289 pKa = 4.85GSFASYY295 pKa = 9.63TISSSGGVLTISSSASGSDD314 pKa = 3.09RR315 pKa = 11.84ISEE318 pKa = 4.07VEE320 pKa = 3.85RR321 pKa = 11.84FQFADD326 pKa = 3.24STRR329 pKa = 11.84TLDD332 pKa = 3.89QLSADD337 pKa = 4.23SDD339 pKa = 3.79TTAPQLVSLNPLDD352 pKa = 5.18DD353 pKa = 4.47GSNVAVGANLVLSFDD368 pKa = 4.08EE369 pKa = 5.53AIKK372 pKa = 10.75AGNGTISIYY381 pKa = 11.05NSDD384 pKa = 3.31GSLARR389 pKa = 11.84SINAVDD395 pKa = 3.44ATQVRR400 pKa = 11.84INGSTVIVDD409 pKa = 4.03PATDD413 pKa = 4.16LLASRR418 pKa = 11.84GYY420 pKa = 10.92YY421 pKa = 8.08ITLSAGALTDD431 pKa = 4.49LADD434 pKa = 3.84NPFAGISGATRR445 pKa = 11.84WNFNTGSTDD454 pKa = 2.79SSAPQIVALTPADD467 pKa = 3.64DD468 pKa = 4.11SGNVARR474 pKa = 11.84NANLVIVFNEE484 pKa = 4.13SVRR487 pKa = 11.84TGSGNLNIRR496 pKa = 11.84DD497 pKa = 3.77ALGEE501 pKa = 4.04LRR503 pKa = 11.84TIAVTDD509 pKa = 4.29ASQVTIDD516 pKa = 4.07GSTVTINPTADD527 pKa = 3.42LAAGASYY534 pKa = 9.78TITVDD539 pKa = 3.0AGAFHH544 pKa = 7.54DD545 pKa = 5.04LAGNPHH551 pKa = 6.49GGIMTTSAWNFSTSATTVTDD571 pKa = 4.35DD572 pKa = 3.74YY573 pKa = 10.92PYY575 pKa = 10.28STDD578 pKa = 3.21TPGLVVVNGASASGTIEE595 pKa = 3.99VPNDD599 pKa = 3.01QDD601 pKa = 3.91LLRR604 pKa = 11.84VEE606 pKa = 4.99LLAGVNYY613 pKa = 9.63TFDD616 pKa = 3.94LQRR619 pKa = 11.84KK620 pKa = 8.82AGGLADD626 pKa = 3.97PFLVLFSPTITQVAFDD642 pKa = 5.02DD643 pKa = 4.67DD644 pKa = 4.5SGGSGNARR652 pKa = 11.84ISYY655 pKa = 7.73TALTTGSYY663 pKa = 10.17YY664 pKa = 10.77LAVVDD669 pKa = 4.23YY670 pKa = 11.17GIGTGGYY677 pKa = 6.43TLRR680 pKa = 11.84ATTADD685 pKa = 3.39SVAPTLVNRR694 pKa = 11.84TPADD698 pKa = 3.36DD699 pKa = 3.83TQAVSVSADD708 pKa = 3.37LVLDD712 pKa = 4.26FSEE715 pKa = 4.7TVLAGSGSLRR725 pKa = 11.84IYY727 pKa = 10.48NSNGSLVRR735 pKa = 11.84EE736 pKa = 4.15IHH738 pKa = 6.49ASDD741 pKa = 3.44SAGVRR746 pKa = 11.84IAGNRR751 pKa = 11.84VTLNPGEE758 pKa = 4.17NLPAGTSFYY767 pKa = 11.8VNIDD771 pKa = 3.05ANAFRR776 pKa = 11.84DD777 pKa = 3.88ASGNTYY783 pKa = 10.9AGLFDD788 pKa = 3.52TSSWNFSTAAVTSTDD803 pKa = 4.64DD804 pKa = 3.71YY805 pKa = 11.26PLSLNTTGMVTVNGPALNARR825 pKa = 11.84IDD827 pKa = 3.63SSNDD831 pKa = 2.53GDD833 pKa = 4.29LFKK836 pKa = 11.09VDD838 pKa = 4.41LSTGVTYY845 pKa = 10.71LFDD848 pKa = 4.44MISPLSSAVDD858 pKa = 3.73PFLVLYY864 pKa = 10.34GMQPEE869 pKa = 4.6VNLITYY875 pKa = 10.39DD876 pKa = 3.88DD877 pKa = 4.85DD878 pKa = 5.38GGPLPLDD885 pKa = 3.12SRR887 pKa = 11.84IYY889 pKa = 7.97YY890 pKa = 8.53TPSTSGTYY898 pKa = 10.42YY899 pKa = 10.81LAAYY903 pKa = 9.49DD904 pKa = 3.91YY905 pKa = 11.77AEE907 pKa = 4.18ATGTYY912 pKa = 9.39SISATVPVDD921 pKa = 3.92DD922 pKa = 4.84YY923 pKa = 11.65LGSTATSGRR932 pKa = 11.84LVSGGAVLTGSIGVPSDD949 pKa = 2.66IDD951 pKa = 3.6MFALSTMAGVQYY963 pKa = 10.94SVDD966 pKa = 3.65LRR968 pKa = 11.84STGLTDD974 pKa = 4.24PYY976 pKa = 11.21LVLLDD981 pKa = 4.3ADD983 pKa = 4.19GTAIAYY989 pKa = 10.1DD990 pKa = 4.19DD991 pKa = 5.21DD992 pKa = 4.89SLGSLNAEE1000 pKa = 3.9ITFTAQTSGTMFLAVSDD1017 pKa = 4.21FDD1019 pKa = 4.24VGTGAYY1025 pKa = 9.76SINAFPRR1032 pKa = 11.84VANITGTSGGDD1043 pKa = 3.49KK1044 pKa = 10.71LIGGADD1050 pKa = 3.28SDD1052 pKa = 4.97LIYY1055 pKa = 11.11GLGGNDD1061 pKa = 3.66TLTGGAGNDD1070 pKa = 3.7TLGGGDD1076 pKa = 4.63GIDD1079 pKa = 3.73TINYY1083 pKa = 7.14GGPASRR1089 pKa = 11.84YY1090 pKa = 7.81VLGVPGTGWSVQDD1103 pKa = 3.6TSSSEE1108 pKa = 4.03GRR1110 pKa = 11.84DD1111 pKa = 3.33LLYY1114 pKa = 10.88DD1115 pKa = 3.34FEE1117 pKa = 6.91RR1118 pKa = 11.84IHH1120 pKa = 7.38FSDD1123 pKa = 3.77YY1124 pKa = 11.34NLAVDD1129 pKa = 4.05IDD1131 pKa = 4.02GHH1133 pKa = 6.92AGTTAKK1139 pKa = 10.45ILGAVFGATAVHH1151 pKa = 5.85NRR1153 pKa = 11.84TYY1155 pKa = 11.26VGIALGLLDD1164 pKa = 5.47GGMSDD1169 pKa = 3.73QSLMQLALDD1178 pKa = 3.91VRR1180 pKa = 11.84LGTAASHH1187 pKa = 5.46QAVASLLYY1195 pKa = 10.23TNVIGSAPPPDD1206 pKa = 3.5ALAYY1210 pKa = 7.61FTSLLDD1216 pKa = 3.83NGVLTPSGLGVLAAEE1231 pKa = 4.61TTQNAVNINLVGLYY1245 pKa = 9.89EE1246 pKa = 4.28SGIEE1250 pKa = 4.16FVV1252 pKa = 4.05

Molecular weight: 129.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A080LYM5|A0A080LYM5_9PROT Uncharacterized protein OS=Candidatus Accumulibacter sp. BA-91 OX=1454002 GN=AW09_000759 PE=4 SV=1
MM1 pKa = 7.67LGQLARR7 pKa = 11.84RR8 pKa = 11.84ARR10 pKa = 11.84VDD12 pKa = 3.21WRR14 pKa = 11.84DD15 pKa = 2.75HH16 pKa = 5.82WGFEE20 pKa = 4.16LLRR23 pKa = 11.84LEE25 pKa = 4.41TFVDD29 pKa = 4.11PRR31 pKa = 11.84HH32 pKa = 6.11HH33 pKa = 7.55AGTCYY38 pKa = 10.21LAAGWQLLGVTSRR51 pKa = 11.84RR52 pKa = 11.84GLARR56 pKa = 11.84PSQSYY61 pKa = 10.47HH62 pKa = 4.3STPRR66 pKa = 11.84QVWVKK71 pKa = 9.53PLTSFRR77 pKa = 11.84LLRR80 pKa = 11.84DD81 pKa = 2.99LDD83 pKa = 4.12LAHH86 pKa = 7.3LEE88 pKa = 4.12QAHH91 pKa = 5.77VDD93 pKa = 3.67LVKK96 pKa = 10.75RR97 pKa = 11.84LIRR100 pKa = 11.84GKK102 pKa = 8.74SFRR105 pKa = 11.84RR106 pKa = 11.84YY107 pKa = 9.9LINHH111 pKa = 7.18CDD113 pKa = 3.88PIAIDD118 pKa = 3.95GSLAIPLGGGTPSAPSARR136 pKa = 11.84TKK138 pKa = 10.48PVTPSICSCSTDD150 pKa = 3.16STPTGRR156 pKa = 4.5

Molecular weight: 17.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4449

0

4449

1187751

29

1810

267.0

29.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.926 ± 0.048

1.19 ± 0.013

5.436 ± 0.034

5.677 ± 0.038

3.604 ± 0.025

7.991 ± 0.041

2.435 ± 0.023

4.737 ± 0.027

2.972 ± 0.032

11.201 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.144 ± 0.018

2.573 ± 0.028

5.062 ± 0.036

4.163 ± 0.025

7.778 ± 0.046

5.588 ± 0.043

4.769 ± 0.035

7.139 ± 0.036

1.349 ± 0.018

2.153 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski