Microbulbifer thermotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Microbulbiferaceae; Microbulbifer

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143HN24|A0A143HN24_9GAMM Cysteine desulfurase IscS OS=Microbulbifer thermotolerans OX=252514 GN=iscS PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 9.45KK3 pKa = 9.21TILRR7 pKa = 11.84RR8 pKa = 11.84AALAAAISTTSSGAMAAGFYY28 pKa = 10.85LNEE31 pKa = 4.14TSISGLGRR39 pKa = 11.84AFAAEE44 pKa = 3.96NTVGDD49 pKa = 3.85NAAILARR56 pKa = 11.84NPAGSALFDD65 pKa = 4.37TITLSGGVIYY75 pKa = 10.1VNPEE79 pKa = 2.82IDD81 pKa = 3.5AEE83 pKa = 4.38GDD85 pKa = 3.42VTYY88 pKa = 9.99FAAPDD93 pKa = 3.74TPIATLSDD101 pKa = 3.6QKK103 pKa = 10.93ATDD106 pKa = 3.81YY107 pKa = 11.59APTAWVPNAYY117 pKa = 10.16LAVPFNEE124 pKa = 3.67NWSFGLAMYY133 pKa = 10.66SNYY136 pKa = 10.58GLEE139 pKa = 4.17TDD141 pKa = 5.88FDD143 pKa = 5.26SDD145 pKa = 3.81WPVTTIADD153 pKa = 3.72KK154 pKa = 11.37TEE156 pKa = 4.0LLTVNIAPSVAYY168 pKa = 10.04DD169 pKa = 3.62FNDD172 pKa = 3.12QFSIGFAVNFLYY184 pKa = 10.84ADD186 pKa = 3.67ATLKK190 pKa = 10.38TMVPTNFGLNPTVAGADD207 pKa = 3.01ILSYY211 pKa = 11.44DD212 pKa = 5.07DD213 pKa = 4.63ADD215 pKa = 3.6DD216 pKa = 4.5WEE218 pKa = 4.6VSWTIGALWNITDD231 pKa = 3.65RR232 pKa = 11.84TRR234 pKa = 11.84FGISYY239 pKa = 9.59HH240 pKa = 6.96AEE242 pKa = 3.88LDD244 pKa = 3.74PEE246 pKa = 4.53LEE248 pKa = 4.28GDD250 pKa = 3.97VNSDD254 pKa = 4.04LVPLPLSPFNDD265 pKa = 3.16AKK267 pKa = 11.33GKK269 pKa = 8.37VTLDD273 pKa = 3.59LPDD276 pKa = 3.69TLEE279 pKa = 4.16LGLYY283 pKa = 10.11HH284 pKa = 7.49RR285 pKa = 11.84FNEE288 pKa = 4.04NWGLALGALWTDD300 pKa = 2.5WDD302 pKa = 3.82DD303 pKa = 4.51FEE305 pKa = 5.98RR306 pKa = 11.84LEE308 pKa = 4.23AFIPAGGEE316 pKa = 4.03GFNPLLFKK324 pKa = 10.98EE325 pKa = 4.88EE326 pKa = 4.08NFQSGWRR333 pKa = 11.84YY334 pKa = 10.32SIAGEE339 pKa = 4.16YY340 pKa = 9.23YY341 pKa = 8.21PCEE344 pKa = 4.03EE345 pKa = 3.77VTLRR349 pKa = 11.84IGYY352 pKa = 10.23AYY354 pKa = 10.33DD355 pKa = 3.18EE356 pKa = 4.69GAARR360 pKa = 11.84DD361 pKa = 3.91GFNTGDD367 pKa = 3.59AAANAYY373 pKa = 8.37GLPITWRR380 pKa = 11.84TLSIPDD386 pKa = 3.6TDD388 pKa = 4.47RR389 pKa = 11.84NWVTIGGTYY398 pKa = 10.36KK399 pKa = 10.66FNNQLSIDD407 pKa = 3.88GGLAYY412 pKa = 10.61LWGDD416 pKa = 3.7DD417 pKa = 3.48EE418 pKa = 5.03TIQEE422 pKa = 4.57FTTVPAPTYY431 pKa = 10.64FDD433 pKa = 4.04GGTTNIEE440 pKa = 3.36AWLAGVSLNYY450 pKa = 10.71AFF452 pKa = 5.5

Molecular weight: 49.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143HR25|A0A143HR25_9GAMM Uncharacterized protein OS=Microbulbifer thermotolerans OX=252514 GN=A3224_15085 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3126

0

3126

1082839

44

3937

346.4

38.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.265 ± 0.052

1.047 ± 0.015

5.707 ± 0.051

6.661 ± 0.038

3.715 ± 0.023

7.941 ± 0.048

2.099 ± 0.023

5.105 ± 0.035

3.75 ± 0.038

10.745 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.023

3.39 ± 0.034

4.647 ± 0.031

4.107 ± 0.031

6.606 ± 0.046

5.929 ± 0.039

4.882 ± 0.033

6.856 ± 0.038

1.434 ± 0.019

2.951 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski