Hubei narna-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIB5|A0A1L3KIB5_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 3 OX=1922957 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 5.32LLKK5 pKa = 10.93YY6 pKa = 8.54PQQRR10 pKa = 11.84VEE12 pKa = 4.65LEE14 pKa = 3.92TSTRR18 pKa = 11.84ALAGLVAQRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 10.62APLRR33 pKa = 11.84TDD35 pKa = 4.69DD36 pKa = 2.96SWLPGRR42 pKa = 11.84ISQIFGAYY50 pKa = 7.86WGPTRR55 pKa = 11.84IEE57 pKa = 3.76EE58 pKa = 4.01RR59 pKa = 11.84LLRR62 pKa = 11.84EE63 pKa = 3.79WEE65 pKa = 3.86AARR68 pKa = 11.84TEE70 pKa = 4.53GIVDD74 pKa = 4.11DD75 pKa = 5.07PCTEE79 pKa = 4.07TDD81 pKa = 4.26SLGNWFRR88 pKa = 11.84IAYY91 pKa = 8.19EE92 pKa = 4.21RR93 pKa = 11.84ALHH96 pKa = 6.04MADD99 pKa = 4.98AYY101 pKa = 9.82QEE103 pKa = 4.21CLLFFDD109 pKa = 5.07EE110 pKa = 5.57KK111 pKa = 11.16NFDD114 pKa = 3.4MSRR117 pKa = 11.84KK118 pKa = 9.67VFFQLVGKK126 pKa = 9.73LFSFGLIGEE135 pKa = 4.37MEE137 pKa = 4.48SYY139 pKa = 11.14LKK141 pKa = 11.01FEE143 pKa = 4.33TATLLAICQEE153 pKa = 4.53SEE155 pKa = 4.31TPPLPEE161 pKa = 3.84YY162 pKa = 10.67LSWNHH167 pKa = 6.07IPGTLFLGGLHH178 pKa = 6.21RR179 pKa = 11.84KK180 pKa = 7.61IWMRR184 pKa = 11.84ARR186 pKa = 11.84HH187 pKa = 5.59RR188 pKa = 11.84RR189 pKa = 11.84RR190 pKa = 11.84TQDD193 pKa = 2.67QSLFYY198 pKa = 10.79SLYY201 pKa = 8.68RR202 pKa = 11.84VKK204 pKa = 10.81DD205 pKa = 3.58VAHH208 pKa = 7.21PLLDD212 pKa = 3.59YY213 pKa = 10.59KK214 pKa = 9.44VQRR217 pKa = 11.84VLKK220 pKa = 10.2RR221 pKa = 11.84NRR223 pKa = 11.84EE224 pKa = 4.06LLTDD228 pKa = 4.01EE229 pKa = 4.81SLHH232 pKa = 6.35PLAATDD238 pKa = 3.89SDD240 pKa = 4.28PAILPGFDD248 pKa = 4.02DD249 pKa = 4.65RR250 pKa = 11.84LSEE253 pKa = 4.23MEE255 pKa = 3.8WQVRR259 pKa = 11.84RR260 pKa = 11.84TVRR263 pKa = 11.84EE264 pKa = 3.68VHH266 pKa = 6.16AMARR270 pKa = 11.84KK271 pKa = 7.51TGWRR275 pKa = 11.84FHH277 pKa = 6.69RR278 pKa = 11.84RR279 pKa = 11.84TPLPTTHH286 pKa = 7.17ASFHH290 pKa = 6.22FSRR293 pKa = 11.84KK294 pKa = 9.29KK295 pKa = 10.45GGAFSEE301 pKa = 4.51LLGSDD306 pKa = 3.82PEE308 pKa = 4.55SPLGFPFLVGVLDD321 pKa = 3.97YY322 pKa = 10.91RR323 pKa = 11.84GSRR326 pKa = 11.84QEE328 pKa = 4.04YY329 pKa = 9.53IYY331 pKa = 10.15TQTDD335 pKa = 3.21PDD337 pKa = 3.66EE338 pKa = 4.64VKK340 pKa = 10.58RR341 pKa = 11.84AMEE344 pKa = 4.11TLPRR348 pKa = 11.84TLDD351 pKa = 3.77YY352 pKa = 10.92ISVRR356 pKa = 11.84SEE358 pKa = 4.31PILEE362 pKa = 4.12PFKK365 pKa = 11.29VRR367 pKa = 11.84VVSCGDD373 pKa = 3.28AVPYY377 pKa = 10.15QLARR381 pKa = 11.84RR382 pKa = 11.84YY383 pKa = 9.69QKK385 pKa = 11.02GLWEE389 pKa = 4.33MLACTDD395 pKa = 3.34TFRR398 pKa = 11.84LVGSPFTEE406 pKa = 4.0HH407 pKa = 7.59DD408 pKa = 3.75VEE410 pKa = 4.56QCCLIPRR417 pKa = 11.84LTGIHH422 pKa = 5.71SVMVSGDD429 pKa = 3.1YY430 pKa = 10.85DD431 pKa = 3.5AATDD435 pKa = 3.71RR436 pKa = 11.84LHH438 pKa = 6.69PHH440 pKa = 7.21LAQSAMDD447 pKa = 3.67EE448 pKa = 4.36FCRR451 pKa = 11.84LEE453 pKa = 4.07GVPFEE458 pKa = 5.48DD459 pKa = 3.85RR460 pKa = 11.84VLLQRR465 pKa = 11.84SLTGHH470 pKa = 5.15TWKK473 pKa = 11.0DD474 pKa = 2.84GDD476 pKa = 3.78RR477 pKa = 11.84QTWGQLMGSFLSFPILNLCNAAEE500 pKa = 4.24TRR502 pKa = 11.84AVMEE506 pKa = 5.12DD507 pKa = 3.6AYY509 pKa = 10.74EE510 pKa = 4.01MYY512 pKa = 10.25IPLEE516 pKa = 3.88WSPLKK521 pKa = 11.28VNGDD525 pKa = 4.46DD526 pKa = 4.71ILFCIPPAAYY536 pKa = 9.23PEE538 pKa = 4.07WSRR541 pKa = 11.84RR542 pKa = 11.84MAQAGLIPSMGKK554 pKa = 9.27NYY556 pKa = 10.07VSRR559 pKa = 11.84RR560 pKa = 11.84YY561 pKa = 10.96AMINSQMYY569 pKa = 8.66EE570 pKa = 4.54LPTDD574 pKa = 3.03WDD576 pKa = 3.7RR577 pKa = 11.84AFRR580 pKa = 11.84GEE582 pKa = 4.36LPHH585 pKa = 7.12LVPTLKK591 pKa = 10.62LGLIRR596 pKa = 11.84GPSSPIQTRR605 pKa = 11.84RR606 pKa = 11.84GLSEE610 pKa = 3.55WMRR613 pKa = 11.84SPEE616 pKa = 4.39CQWSPYY622 pKa = 10.49NLGDD626 pKa = 4.28QMRR629 pKa = 11.84EE630 pKa = 3.61AAKK633 pKa = 10.87GFGRR637 pKa = 11.84DD638 pKa = 3.26MASRR642 pKa = 11.84IYY644 pKa = 10.7SSSLRR649 pKa = 11.84YY650 pKa = 9.46ALPLLQQLPPVSWFSHH666 pKa = 5.77PSKK669 pKa = 10.89GGLGLPTDD677 pKa = 4.8RR678 pKa = 11.84DD679 pKa = 3.86PEE681 pKa = 4.26SLIRR685 pKa = 11.84EE686 pKa = 3.97HH687 pKa = 6.63HH688 pKa = 6.07RR689 pKa = 11.84RR690 pKa = 11.84IASFLTCHH698 pKa = 6.46NFTHH702 pKa = 6.32PHH704 pKa = 5.89EE705 pKa = 4.29EE706 pKa = 4.15QIRR709 pKa = 11.84HH710 pKa = 5.96SIRR713 pKa = 11.84MSGSPLYY720 pKa = 10.77ASAAIAAVQDD730 pKa = 3.29SCDD733 pKa = 3.47RR734 pKa = 11.84LRR736 pKa = 11.84IPYY739 pKa = 8.37EE740 pKa = 4.04KK741 pKa = 10.57KK742 pKa = 10.66SILLDD747 pKa = 3.54KK748 pKa = 10.55EE749 pKa = 4.02EE750 pKa = 4.54CRR752 pKa = 11.84VEE754 pKa = 4.37SSLLSKK760 pKa = 9.78QLWGNSRR767 pKa = 11.84SCTALEE773 pKa = 4.17NDD775 pKa = 5.03DD776 pKa = 5.3DD777 pKa = 6.55RR778 pKa = 11.84EE779 pKa = 4.23GDD781 pKa = 3.92WIRR784 pKa = 11.84DD785 pKa = 3.33WTRR788 pKa = 11.84SYY790 pKa = 10.54WKK792 pKa = 7.89WTRR795 pKa = 11.84LAEE798 pKa = 4.34SVVRR802 pKa = 11.84PGCLLPMNAMNCILPWEE819 pKa = 4.42FRR821 pKa = 11.84WEE823 pKa = 3.89RR824 pKa = 11.84DD825 pKa = 3.24FSGVILTT832 pKa = 4.4

Molecular weight: 96.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIB5|A0A1L3KIB5_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 3 OX=1922957 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 5.32LLKK5 pKa = 10.93YY6 pKa = 8.54PQQRR10 pKa = 11.84VEE12 pKa = 4.65LEE14 pKa = 3.92TSTRR18 pKa = 11.84ALAGLVAQRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 10.62APLRR33 pKa = 11.84TDD35 pKa = 4.69DD36 pKa = 2.96SWLPGRR42 pKa = 11.84ISQIFGAYY50 pKa = 7.86WGPTRR55 pKa = 11.84IEE57 pKa = 3.76EE58 pKa = 4.01RR59 pKa = 11.84LLRR62 pKa = 11.84EE63 pKa = 3.79WEE65 pKa = 3.86AARR68 pKa = 11.84TEE70 pKa = 4.53GIVDD74 pKa = 4.11DD75 pKa = 5.07PCTEE79 pKa = 4.07TDD81 pKa = 4.26SLGNWFRR88 pKa = 11.84IAYY91 pKa = 8.19EE92 pKa = 4.21RR93 pKa = 11.84ALHH96 pKa = 6.04MADD99 pKa = 4.98AYY101 pKa = 9.82QEE103 pKa = 4.21CLLFFDD109 pKa = 5.07EE110 pKa = 5.57KK111 pKa = 11.16NFDD114 pKa = 3.4MSRR117 pKa = 11.84KK118 pKa = 9.67VFFQLVGKK126 pKa = 9.73LFSFGLIGEE135 pKa = 4.37MEE137 pKa = 4.48SYY139 pKa = 11.14LKK141 pKa = 11.01FEE143 pKa = 4.33TATLLAICQEE153 pKa = 4.53SEE155 pKa = 4.31TPPLPEE161 pKa = 3.84YY162 pKa = 10.67LSWNHH167 pKa = 6.07IPGTLFLGGLHH178 pKa = 6.21RR179 pKa = 11.84KK180 pKa = 7.61IWMRR184 pKa = 11.84ARR186 pKa = 11.84HH187 pKa = 5.59RR188 pKa = 11.84RR189 pKa = 11.84RR190 pKa = 11.84TQDD193 pKa = 2.67QSLFYY198 pKa = 10.79SLYY201 pKa = 8.68RR202 pKa = 11.84VKK204 pKa = 10.81DD205 pKa = 3.58VAHH208 pKa = 7.21PLLDD212 pKa = 3.59YY213 pKa = 10.59KK214 pKa = 9.44VQRR217 pKa = 11.84VLKK220 pKa = 10.2RR221 pKa = 11.84NRR223 pKa = 11.84EE224 pKa = 4.06LLTDD228 pKa = 4.01EE229 pKa = 4.81SLHH232 pKa = 6.35PLAATDD238 pKa = 3.89SDD240 pKa = 4.28PAILPGFDD248 pKa = 4.02DD249 pKa = 4.65RR250 pKa = 11.84LSEE253 pKa = 4.23MEE255 pKa = 3.8WQVRR259 pKa = 11.84RR260 pKa = 11.84TVRR263 pKa = 11.84EE264 pKa = 3.68VHH266 pKa = 6.16AMARR270 pKa = 11.84KK271 pKa = 7.51TGWRR275 pKa = 11.84FHH277 pKa = 6.69RR278 pKa = 11.84RR279 pKa = 11.84TPLPTTHH286 pKa = 7.17ASFHH290 pKa = 6.22FSRR293 pKa = 11.84KK294 pKa = 9.29KK295 pKa = 10.45GGAFSEE301 pKa = 4.51LLGSDD306 pKa = 3.82PEE308 pKa = 4.55SPLGFPFLVGVLDD321 pKa = 3.97YY322 pKa = 10.91RR323 pKa = 11.84GSRR326 pKa = 11.84QEE328 pKa = 4.04YY329 pKa = 9.53IYY331 pKa = 10.15TQTDD335 pKa = 3.21PDD337 pKa = 3.66EE338 pKa = 4.64VKK340 pKa = 10.58RR341 pKa = 11.84AMEE344 pKa = 4.11TLPRR348 pKa = 11.84TLDD351 pKa = 3.77YY352 pKa = 10.92ISVRR356 pKa = 11.84SEE358 pKa = 4.31PILEE362 pKa = 4.12PFKK365 pKa = 11.29VRR367 pKa = 11.84VVSCGDD373 pKa = 3.28AVPYY377 pKa = 10.15QLARR381 pKa = 11.84RR382 pKa = 11.84YY383 pKa = 9.69QKK385 pKa = 11.02GLWEE389 pKa = 4.33MLACTDD395 pKa = 3.34TFRR398 pKa = 11.84LVGSPFTEE406 pKa = 4.0HH407 pKa = 7.59DD408 pKa = 3.75VEE410 pKa = 4.56QCCLIPRR417 pKa = 11.84LTGIHH422 pKa = 5.71SVMVSGDD429 pKa = 3.1YY430 pKa = 10.85DD431 pKa = 3.5AATDD435 pKa = 3.71RR436 pKa = 11.84LHH438 pKa = 6.69PHH440 pKa = 7.21LAQSAMDD447 pKa = 3.67EE448 pKa = 4.36FCRR451 pKa = 11.84LEE453 pKa = 4.07GVPFEE458 pKa = 5.48DD459 pKa = 3.85RR460 pKa = 11.84VLLQRR465 pKa = 11.84SLTGHH470 pKa = 5.15TWKK473 pKa = 11.0DD474 pKa = 2.84GDD476 pKa = 3.78RR477 pKa = 11.84QTWGQLMGSFLSFPILNLCNAAEE500 pKa = 4.24TRR502 pKa = 11.84AVMEE506 pKa = 5.12DD507 pKa = 3.6AYY509 pKa = 10.74EE510 pKa = 4.01MYY512 pKa = 10.25IPLEE516 pKa = 3.88WSPLKK521 pKa = 11.28VNGDD525 pKa = 4.46DD526 pKa = 4.71ILFCIPPAAYY536 pKa = 9.23PEE538 pKa = 4.07WSRR541 pKa = 11.84RR542 pKa = 11.84MAQAGLIPSMGKK554 pKa = 9.27NYY556 pKa = 10.07VSRR559 pKa = 11.84RR560 pKa = 11.84YY561 pKa = 10.96AMINSQMYY569 pKa = 8.66EE570 pKa = 4.54LPTDD574 pKa = 3.03WDD576 pKa = 3.7RR577 pKa = 11.84AFRR580 pKa = 11.84GEE582 pKa = 4.36LPHH585 pKa = 7.12LVPTLKK591 pKa = 10.62LGLIRR596 pKa = 11.84GPSSPIQTRR605 pKa = 11.84RR606 pKa = 11.84GLSEE610 pKa = 3.55WMRR613 pKa = 11.84SPEE616 pKa = 4.39CQWSPYY622 pKa = 10.49NLGDD626 pKa = 4.28QMRR629 pKa = 11.84EE630 pKa = 3.61AAKK633 pKa = 10.87GFGRR637 pKa = 11.84DD638 pKa = 3.26MASRR642 pKa = 11.84IYY644 pKa = 10.7SSSLRR649 pKa = 11.84YY650 pKa = 9.46ALPLLQQLPPVSWFSHH666 pKa = 5.77PSKK669 pKa = 10.89GGLGLPTDD677 pKa = 4.8RR678 pKa = 11.84DD679 pKa = 3.86PEE681 pKa = 4.26SLIRR685 pKa = 11.84EE686 pKa = 3.97HH687 pKa = 6.63HH688 pKa = 6.07RR689 pKa = 11.84RR690 pKa = 11.84IASFLTCHH698 pKa = 6.46NFTHH702 pKa = 6.32PHH704 pKa = 5.89EE705 pKa = 4.29EE706 pKa = 4.15QIRR709 pKa = 11.84HH710 pKa = 5.96SIRR713 pKa = 11.84MSGSPLYY720 pKa = 10.77ASAAIAAVQDD730 pKa = 3.29SCDD733 pKa = 3.47RR734 pKa = 11.84LRR736 pKa = 11.84IPYY739 pKa = 8.37EE740 pKa = 4.04KK741 pKa = 10.57KK742 pKa = 10.66SILLDD747 pKa = 3.54KK748 pKa = 10.55EE749 pKa = 4.02EE750 pKa = 4.54CRR752 pKa = 11.84VEE754 pKa = 4.37SSLLSKK760 pKa = 9.78QLWGNSRR767 pKa = 11.84SCTALEE773 pKa = 4.17NDD775 pKa = 5.03DD776 pKa = 5.3DD777 pKa = 6.55RR778 pKa = 11.84EE779 pKa = 4.23GDD781 pKa = 3.92WIRR784 pKa = 11.84DD785 pKa = 3.33WTRR788 pKa = 11.84SYY790 pKa = 10.54WKK792 pKa = 7.89WTRR795 pKa = 11.84LAEE798 pKa = 4.34SVVRR802 pKa = 11.84PGCLLPMNAMNCILPWEE819 pKa = 4.42FRR821 pKa = 11.84WEE823 pKa = 3.89RR824 pKa = 11.84DD825 pKa = 3.24FSGVILTT832 pKa = 4.4

Molecular weight: 96.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

832

832

832

832.0

96.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.37 ± 0.0

2.043 ± 0.0

6.01 ± 0.0

6.971 ± 0.0

4.087 ± 0.0

5.769 ± 0.0

2.764 ± 0.0

4.087 ± 0.0

3.245 ± 0.0

11.298 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.885 ± 0.0

1.803 ± 0.0

6.37 ± 0.0

3.606 ± 0.0

9.495 ± 0.0

7.572 ± 0.0

5.048 ± 0.0

4.327 ± 0.0

2.885 ± 0.0

3.365 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski