Ascobolus immersus RN42

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Pezizomycetes; Pezizales; Ascobolaceae; Ascobolus; Ascobolus immersus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N4HX14|A0A3N4HX14_ASCIM F-box domain-containing protein OS=Ascobolus immersus RN42 OX=1160509 GN=BJ508DRAFT_156332 PE=4 SV=1
MM1 pKa = 7.55PRR3 pKa = 11.84DD4 pKa = 3.75STPPLSPDD12 pKa = 3.31LSTFSPALSSRR23 pKa = 11.84PTFDD27 pKa = 4.44FDD29 pKa = 4.58GSLPDD34 pKa = 3.58LVEE37 pKa = 4.63DD38 pKa = 3.6VTHH41 pKa = 7.24FLTCGHH47 pKa = 6.94CGASDD52 pKa = 3.77DD53 pKa = 4.52PEE55 pKa = 5.79LEE57 pKa = 4.03VVEE60 pKa = 4.39THH62 pKa = 6.69EE63 pKa = 5.22GCCPYY68 pKa = 10.41CPHH71 pKa = 6.43YY72 pKa = 10.13WVGDD76 pKa = 3.84HH77 pKa = 7.05LPPPLQQALAPPAEE91 pKa = 4.95DD92 pKa = 3.69SSHH95 pKa = 5.52VPLWRR100 pKa = 11.84ADD102 pKa = 3.85RR103 pKa = 11.84AGSQSPLMLPGFWTPGPSRR122 pKa = 11.84SPSPEE127 pKa = 3.87PSPATPSSPDD137 pKa = 3.36EE138 pKa = 4.23PQSPQEE144 pKa = 4.1APGGPGMSVLMDD156 pKa = 3.52IDD158 pKa = 4.13GQDD161 pKa = 3.55EE162 pKa = 4.41EE163 pKa = 5.84GDD165 pKa = 3.91EE166 pKa = 4.7ADD168 pKa = 5.54LPLAPPSSPGDD179 pKa = 3.29TTMGDD184 pKa = 3.51EE185 pKa = 4.17

Molecular weight: 19.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N4I1G5|A0A3N4I1G5_ASCIM Alkyl transferase OS=Ascobolus immersus RN42 OX=1160509 GN=BJ508DRAFT_416778 PE=3 SV=1
MM1 pKa = 7.43WLISAPNSSILVTPLPSALSRR22 pKa = 11.84LPLLPPGSFRR32 pKa = 11.84SLQFPSAPSRR42 pKa = 11.84FPPLPPGSLRR52 pKa = 11.84SLQVHH57 pKa = 5.94SAPSRR62 pKa = 11.84FPPLPPGSLRR72 pKa = 11.84SLQVPSAPSRR82 pKa = 11.84FPPLPPGSLRR92 pKa = 11.84SLQVSSVPSRR102 pKa = 11.84FLPFPPGSLRR112 pKa = 11.84SLQVPSAALNKK123 pKa = 9.69TSAA126 pKa = 3.63

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17778

0

17778

7298591

49

10312

410.5

45.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.734 ± 0.015

1.359 ± 0.007

5.578 ± 0.014

7.066 ± 0.022

3.667 ± 0.012

6.449 ± 0.019

2.446 ± 0.008

4.666 ± 0.013

5.483 ± 0.019

8.654 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.079 ± 0.007

3.626 ± 0.011

6.477 ± 0.021

3.907 ± 0.017

6.457 ± 0.018

8.099 ± 0.021

6.216 ± 0.02

5.86 ± 0.012

1.391 ± 0.007

2.785 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski