Amino acid dipepetide frequency for Thermococcus sibiricus (strain DSM 12597 / MM 739)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.872AlaAla: 3.872 ± 0.111	0.427AlaCys: 0.427 ± 0.033	2.573AlaAsp: 2.573 ± 0.071	4.81AlaGlu: 4.81 ± 0.103	3.35AlaPhe: 3.35 ± 0.076	4.356AlaGly: 4.356 ± 0.103	1.242AlaHis: 1.242 ± 0.048	6.169AlaIle: 6.169 ± 0.132	5.796AlaLys: 5.796 ± 0.107	8.489AlaLeu: 8.489 ± 0.155	1.893AlaMet: 1.893 ± 0.065	2.016AlaAsn: 2.016 ± 0.062	2.203AlaPro: 2.203 ± 0.071	1.559AlaGln: 1.559 ± 0.057	3.303AlaArg: 3.303 ± 0.072	3.204AlaSer: 3.204 ± 0.083	2.966AlaThr: 2.966 ± 0.082	4.981AlaVal: 4.981 ± 0.112	0.761AlaTrp: 0.761 ± 0.041	2.845AlaTyr: 2.845 ± 0.082	0.0AlaXaa: 0.0 ± 0.0
Cys	0.323CysAla: 0.323 ± 0.025	0.047CysCys: 0.047 ± 0.011	0.294CysAsp: 0.294 ± 0.022	0.398CysGlu: 0.398 ± 0.027	0.187CysPhe: 0.187 ± 0.016	0.759CysGly: 0.759 ± 0.046	0.121CysHis: 0.121 ± 0.014	0.371CysIle: 0.371 ± 0.031	0.368CysLys: 0.368 ± 0.027	0.415CysLeu: 0.415 ± 0.029	0.13CysMet: 0.13 ± 0.014	0.204CysAsn: 0.204 ± 0.019	0.66CysPro: 0.66 ± 0.046	0.148CysGln: 0.148 ± 0.017	0.276CysArg: 0.276 ± 0.025	0.334CysSer: 0.334 ± 0.029	0.265CysThr: 0.265 ± 0.023	0.344CysVal: 0.344 ± 0.028	0.07CysTrp: 0.07 ± 0.011	0.171CysTyr: 0.171 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	2.803AspAla: 2.803 ± 0.072	0.197AspCys: 0.197 ± 0.022	1.886AspAsp: 1.886 ± 0.063	4.347AspGlu: 4.347 ± 0.081	2.383AspPhe: 2.383 ± 0.065	3.086AspGly: 3.086 ± 0.085	0.685AspHis: 0.685 ± 0.034	4.173AspIle: 4.173 ± 0.077	3.025AspLys: 3.025 ± 0.076	4.792AspLeu: 4.792 ± 0.088	1.096AspMet: 1.096 ± 0.045	1.397AspAsn: 1.397 ± 0.053	2.241AspPro: 2.241 ± 0.068	0.69AspGln: 0.69 ± 0.041	1.864AspArg: 1.864 ± 0.061	1.92AspSer: 1.92 ± 0.064	1.81AspThr: 1.81 ± 0.058	4.123AspVal: 4.123 ± 0.09	0.635AspTrp: 0.635 ± 0.036	2.18AspTyr: 2.18 ± 0.069	0.0AspXaa: 0.0 ± 0.0
Glu	5.376GluAla: 5.376 ± 0.099	0.379GluCys: 0.379 ± 0.03	4.253GluAsp: 4.253 ± 0.098	9.578GluGlu: 9.578 ± 0.172	3.872GluPhe: 3.872 ± 0.092	5.554GluGly: 5.554 ± 0.104	1.403GluHis: 1.403 ± 0.055	8.289GluIle: 8.289 ± 0.154	8.985GluLys: 8.985 ± 0.165	8.967GluLeu: 8.967 ± 0.145	2.171GluMet: 2.171 ± 0.064	4.009GluAsn: 4.009 ± 0.089	2.437GluPro: 2.437 ± 0.065	1.347GluGln: 1.347 ± 0.055	4.662GluArg: 4.662 ± 0.099	3.276GluSer: 3.276 ± 0.075	3.25GluThr: 3.25 ± 0.086	6.95GluVal: 6.95 ± 0.107	1.042GluTrp: 1.042 ± 0.039	3.113GluTyr: 3.113 ± 0.087	0.0GluXaa: 0.0 ± 0.0
Phe	3.007PheAla: 3.007 ± 0.077	0.24PheCys: 0.24 ± 0.022	2.347PheAsp: 2.347 ± 0.06	3.932PheGlu: 3.932 ± 0.081	2.007PhePhe: 2.007 ± 0.072	3.867PheGly: 3.867 ± 0.096	0.718PheHis: 0.718 ± 0.039	3.431PheIle: 3.431 ± 0.08	3.133PheLys: 3.133 ± 0.079	4.862PheLeu: 4.862 ± 0.118	1.157PheMet: 1.157 ± 0.053	1.639PheAsn: 1.639 ± 0.059	1.731PhePro: 1.731 ± 0.061	0.804PheGln: 0.804 ± 0.035	1.933PheArg: 1.933 ± 0.06	2.607PheSer: 2.607 ± 0.071	1.985PheThr: 1.985 ± 0.06	3.337PheVal: 3.337 ± 0.075	0.55PheTrp: 0.55 ± 0.033	1.873PheTyr: 1.873 ± 0.061	0.0PheXaa: 0.0 ± 0.0
Gly	4.85GlyAla: 4.85 ± 0.115	0.548GlyCys: 0.548 ± 0.035	3.411GlyAsp: 3.411 ± 0.09	5.709GlyGlu: 5.709 ± 0.107	3.591GlyPhe: 3.591 ± 0.079	4.934GlyGly: 4.934 ± 0.124	1.21GlyHis: 1.21 ± 0.049	6.901GlyIle: 6.901 ± 0.124	6.149GlyLys: 6.149 ± 0.109	6.632GlyLeu: 6.632 ± 0.122	2.003GlyMet: 2.003 ± 0.07	2.518GlyAsn: 2.518 ± 0.083	1.846GlyPro: 1.846 ± 0.064	1.258GlyGln: 1.258 ± 0.054	3.407GlyArg: 3.407 ± 0.084	3.351GlySer: 3.351 ± 0.076	3.434GlyThr: 3.434 ± 0.089	5.895GlyVal: 5.895 ± 0.107	0.914GlyTrp: 0.914 ± 0.041	3.175GlyTyr: 3.175 ± 0.082	0.0GlyXaa: 0.0 ± 0.0
His	1.069HisAla: 1.069 ± 0.045	0.151HisCys: 0.151 ± 0.017	0.705HisAsp: 0.705 ± 0.032	1.23HisGlu: 1.23 ± 0.052	0.802HisPhe: 0.802 ± 0.036	1.271HisGly: 1.271 ± 0.053	0.386HisHis: 0.386 ± 0.028	1.276HisIle: 1.276 ± 0.054	1.024HisLys: 1.024 ± 0.047	1.778HisLeu: 1.778 ± 0.055	0.344HisMet: 0.344 ± 0.023	0.535HisAsn: 0.535 ± 0.035	1.006HisPro: 1.006 ± 0.046	0.312HisGln: 0.312 ± 0.024	0.811HisArg: 0.811 ± 0.04	0.829HisSer: 0.829 ± 0.035	0.696HisThr: 0.696 ± 0.034	1.208HisVal: 1.208 ± 0.047	0.227HisTrp: 0.227 ± 0.02	0.838HisTyr: 0.838 ± 0.044	0.0HisXaa: 0.0 ± 0.0
Ile	6.49IleAla: 6.49 ± 0.111	0.451IleCys: 0.451 ± 0.031	3.901IleAsp: 3.901 ± 0.081	7.519IleGlu: 7.519 ± 0.126	3.523IlePhe: 3.523 ± 0.084	6.261IleGly: 6.261 ± 0.126	1.356IleHis: 1.356 ± 0.053	6.851IleIle: 6.851 ± 0.127	6.977IleLys: 6.977 ± 0.123	8.39IleLeu: 8.39 ± 0.144	1.938IleMet: 1.938 ± 0.064	3.016IleAsn: 3.016 ± 0.073	4.071IlePro: 4.071 ± 0.082	1.709IleGln: 1.709 ± 0.057	3.939IleArg: 3.939 ± 0.093	4.635IleSer: 4.635 ± 0.099	4.249IleThr: 4.249 ± 0.094	6.088IleVal: 6.088 ± 0.094	0.764IleTrp: 0.764 ± 0.042	2.915IleTyr: 2.915 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	5.976LysAla: 5.976 ± 0.104	0.571LysCys: 0.571 ± 0.038	3.854LysAsp: 3.854 ± 0.088	8.859LysGlu: 8.859 ± 0.165	3.052LysPhe: 3.052 ± 0.075	5.369LysGly: 5.369 ± 0.114	1.156LysHis: 1.156 ± 0.044	7.213LysIle: 7.213 ± 0.125	7.036LysLys: 7.036 ± 0.153	7.676LysLeu: 7.676 ± 0.127	2.034LysMet: 2.034 ± 0.056	3.276LysAsn: 3.276 ± 0.086	3.041LysPro: 3.041 ± 0.072	1.417LysGln: 1.417 ± 0.053	4.487LysArg: 4.487 ± 0.117	3.36LysSer: 3.36 ± 0.079	3.696LysThr: 3.696 ± 0.093	6.474LysVal: 6.474 ± 0.108	1.056LysTrp: 1.056 ± 0.049	3.009LysTyr: 3.009 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	7.33LeuAla: 7.33 ± 0.137	0.485LeuCys: 0.485 ± 0.033	4.579LeuAsp: 4.579 ± 0.107	8.953LeuGlu: 8.953 ± 0.148	4.148LeuPhe: 4.148 ± 0.099	7.92LeuGly: 7.92 ± 0.144	1.498LeuHis: 1.498 ± 0.057	8.071LeuIle: 8.071 ± 0.131	9.063LeuLys: 9.063 ± 0.143	9.987LeuLeu: 9.987 ± 0.161	2.533LeuMet: 2.533 ± 0.063	3.842LeuAsn: 3.842 ± 0.102	4.191LeuPro: 4.191 ± 0.104	2.064LeuGln: 2.064 ± 0.066	5.187LeuArg: 5.187 ± 0.098	5.893LeuSer: 5.893 ± 0.108	4.658LeuThr: 4.658 ± 0.107	6.881LeuVal: 6.881 ± 0.123	1.082LeuTrp: 1.082 ± 0.046	3.517LeuTyr: 3.517 ± 0.085	0.0LeuXaa: 0.0 ± 0.0
Met	1.808MetAla: 1.808 ± 0.06	0.114MetCys: 0.114 ± 0.014	1.02MetAsp: 1.02 ± 0.039	2.129MetGlu: 2.129 ± 0.062	0.939MetPhe: 0.939 ± 0.046	1.81MetGly: 1.81 ± 0.067	0.402MetHis: 0.402 ± 0.024	1.96MetIle: 1.96 ± 0.065	2.363MetLys: 2.363 ± 0.067	2.41MetLeu: 2.41 ± 0.065	0.553MetMet: 0.553 ± 0.032	0.995MetAsn: 0.995 ± 0.037	1.083MetPro: 1.083 ± 0.049	0.49MetGln: 0.49 ± 0.031	1.431MetArg: 1.431 ± 0.046	1.233MetSer: 1.233 ± 0.049	0.993MetThr: 0.993 ± 0.048	1.783MetVal: 1.783 ± 0.053	0.224MetTrp: 0.224 ± 0.02	0.662MetTyr: 0.662 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	2.659AsnAla: 2.659 ± 0.07	0.222AsnCys: 0.222 ± 0.02	1.359AsnAsp: 1.359 ± 0.05	2.984AsnGlu: 2.984 ± 0.084	1.707AsnPhe: 1.707 ± 0.051	2.778AsnGly: 2.778 ± 0.092	0.546AsnHis: 0.546 ± 0.033	3.146AsnIle: 3.146 ± 0.072	2.311AsnLys: 2.311 ± 0.081	4.006AsnLeu: 4.006 ± 0.084	0.788AsnMet: 0.788 ± 0.035	1.206AsnAsn: 1.206 ± 0.048	2.36AsnPro: 2.36 ± 0.066	0.696AsnGln: 0.696 ± 0.035	1.422AsnArg: 1.422 ± 0.056	1.823AsnSer: 1.823 ± 0.061	1.595AsnThr: 1.595 ± 0.052	2.994AsnVal: 2.994 ± 0.067	0.44AsnTrp: 0.44 ± 0.03	1.482AsnTyr: 1.482 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.205ProAla: 2.205 ± 0.068	0.247ProCys: 0.247 ± 0.022	1.907ProAsp: 1.907 ± 0.052	4.071ProGlu: 4.071 ± 0.091	1.902ProPhe: 1.902 ± 0.054	2.542ProGly: 2.542 ± 0.075	0.874ProHis: 0.874 ± 0.038	3.369ProIle: 3.369 ± 0.079	3.353ProLys: 3.353 ± 0.088	3.952ProLeu: 3.952 ± 0.099	0.918ProMet: 0.918 ± 0.043	1.552ProAsn: 1.552 ± 0.052	1.7ProPro: 1.7 ± 0.059	1.083ProGln: 1.083 ± 0.042	1.844ProArg: 1.844 ± 0.062	2.104ProSer: 2.104 ± 0.067	2.088ProThr: 2.088 ± 0.058	2.814ProVal: 2.814 ± 0.08	0.503ProTrp: 0.503 ± 0.03	1.824ProTyr: 1.824 ± 0.061	0.0ProXaa: 0.0 ± 0.0
Gln	1.403GlnAla: 1.403 ± 0.054	0.128GlnCys: 0.128 ± 0.018	0.764GlnAsp: 0.764 ± 0.036	1.714GlnGlu: 1.714 ± 0.058	0.795GlnPhe: 0.795 ± 0.04	1.264GlnGly: 1.264 ± 0.04	0.332GlnHis: 0.332 ± 0.027	1.788GlnIle: 1.788 ± 0.056	1.947GlnLys: 1.947 ± 0.066	1.792GlnLeu: 1.792 ± 0.059	0.627GlnMet: 0.627 ± 0.034	0.867GlnAsn: 0.867 ± 0.042	0.595GlnPro: 0.595 ± 0.035	0.49GlnGln: 0.49 ± 0.031	1.235GlnArg: 1.235 ± 0.05	0.844GlnSer: 0.844 ± 0.042	0.903GlnThr: 0.903 ± 0.034	1.372GlnVal: 1.372 ± 0.052	0.222GlnTrp: 0.222 ± 0.021	0.734GlnTyr: 0.734 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.195ArgAla: 3.195 ± 0.079	0.332ArgCys: 0.332 ± 0.027	2.284ArgAsp: 2.284 ± 0.064	5.226ArgGlu: 5.226 ± 0.107	2.205ArgPhe: 2.205 ± 0.064	3.404ArgGly: 3.404 ± 0.084	0.739ArgHis: 0.739 ± 0.034	4.222ArgIle: 4.222 ± 0.084	4.303ArgLys: 4.303 ± 0.095	4.298ArgLeu: 4.298 ± 0.097	1.213ArgMet: 1.213 ± 0.046	1.7ArgAsn: 1.7 ± 0.057	1.709ArgPro: 1.709 ± 0.061	0.838ArgGln: 0.838 ± 0.038	2.796ArgArg: 2.796 ± 0.08	1.869ArgSer: 1.869 ± 0.06	2.014ArgThr: 2.014 ± 0.055	3.914ArgVal: 3.914 ± 0.089	0.534ArgTrp: 0.534 ± 0.033	1.869ArgTyr: 1.869 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	2.969SerAla: 2.969 ± 0.08	0.305SerCys: 0.305 ± 0.026	2.053SerAsp: 2.053 ± 0.061	3.874SerGlu: 3.874 ± 0.094	2.56SerPhe: 2.56 ± 0.073	3.849SerGly: 3.849 ± 0.091	0.903SerHis: 0.903 ± 0.044	4.06SerIle: 4.06 ± 0.093	3.703SerLys: 3.703 ± 0.079	5.538SerLeu: 5.538 ± 0.103	1.222SerMet: 1.222 ± 0.041	1.7SerAsn: 1.7 ± 0.061	2.154SerPro: 2.154 ± 0.066	1.244SerGln: 1.244 ± 0.042	2.199SerArg: 2.199 ± 0.063	2.784SerSer: 2.784 ± 0.082	2.43SerThr: 2.43 ± 0.069	3.101SerVal: 3.101 ± 0.08	0.579SerTrp: 0.579 ± 0.033	2.005SerTyr: 2.005 ± 0.065	0.0SerXaa: 0.0 ± 0.0
Thr	3.056ThrAla: 3.056 ± 0.074	0.283ThrCys: 0.283 ± 0.025	1.714ThrAsp: 1.714 ± 0.057	2.764ThrGlu: 2.764 ± 0.071	2.234ThrPhe: 2.234 ± 0.073	3.496ThrGly: 3.496 ± 0.081	0.826ThrHis: 0.826 ± 0.036	3.737ThrIle: 3.737 ± 0.08	3.07ThrLys: 3.07 ± 0.078	5.138ThrLeu: 5.138 ± 0.111	0.934ThrMet: 0.934 ± 0.04	1.585ThrAsn: 1.585 ± 0.061	2.526ThrPro: 2.526 ± 0.073	1.042ThrGln: 1.042 ± 0.045	1.891ThrArg: 1.891 ± 0.063	2.549ThrSer: 2.549 ± 0.074	2.524ThrThr: 2.524 ± 0.098	3.187ThrVal: 3.187 ± 0.084	0.604ThrTrp: 0.604 ± 0.035	1.832ThrTyr: 1.832 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	5.019ValAla: 5.019 ± 0.116	0.415ValCys: 0.415 ± 0.031	3.847ValAsp: 3.847 ± 0.092	6.6ValGlu: 6.6 ± 0.114	3.514ValPhe: 3.514 ± 0.084	5.371ValGly: 5.371 ± 0.12	1.172ValHis: 1.172 ± 0.041	6.285ValIle: 6.285 ± 0.104	6.364ValLys: 6.364 ± 0.121	7.437ValLeu: 7.437 ± 0.134	1.696ValMet: 1.696 ± 0.061	2.468ValAsn: 2.468 ± 0.061	3.088ValPro: 3.088 ± 0.068	1.487ValGln: 1.487 ± 0.057	3.562ValArg: 3.562 ± 0.085	3.795ValSer: 3.795 ± 0.077	3.186ValThr: 3.186 ± 0.08	5.8ValVal: 5.8 ± 0.123	0.737ValTrp: 0.737 ± 0.04	2.902ValTyr: 2.902 ± 0.069	0.0ValXaa: 0.0 ± 0.0
Trp	0.752TrpAla: 0.752 ± 0.04	0.072TrpCys: 0.072 ± 0.012	0.656TrpAsp: 0.656 ± 0.032	1.103TrpGlu: 1.103 ± 0.05	0.483TrpPhe: 0.483 ± 0.035	0.874TrpGly: 0.874 ± 0.039	0.233TrpHis: 0.233 ± 0.017	0.934TrpIle: 0.934 ± 0.045	1.04TrpLys: 1.04 ± 0.046	1.002TrpLeu: 1.002 ± 0.046	0.325TrpMet: 0.325 ± 0.028	0.49TrpAsn: 0.49 ± 0.035	0.315TrpPro: 0.315 ± 0.025	0.294TrpGln: 0.294 ± 0.033	0.629TrpArg: 0.629 ± 0.035	0.546TrpSer: 0.546 ± 0.033	0.409TrpThr: 0.409 ± 0.026	0.883TrpVal: 0.883 ± 0.051	0.24TrpTrp: 0.24 ± 0.024	0.436TrpTyr: 0.436 ± 0.031	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.652TyrAla: 2.652 ± 0.075	0.272TyrCys: 0.272 ± 0.025	1.924TyrAsp: 1.924 ± 0.062	3.113TyrGlu: 3.113 ± 0.077	1.904TyrPhe: 1.904 ± 0.061	2.975TyrGly: 2.975 ± 0.08	0.699TyrHis: 0.699 ± 0.036	2.796TyrIle: 2.796 ± 0.082	2.601TyrLys: 2.601 ± 0.076	4.321TyrLeu: 4.321 ± 0.089	0.827TyrMet: 0.827 ± 0.042	1.509TyrAsn: 1.509 ± 0.053	1.895TyrPro: 1.895 ± 0.058	0.842TyrGln: 0.842 ± 0.039	1.785TyrArg: 1.785 ± 0.053	2.279TyrSer: 2.279 ± 0.073	1.839TyrThr: 1.839 ± 0.064	2.614TyrVal: 2.614 ± 0.075	0.535TyrTrp: 0.535 ± 0.03	1.792TyrTyr: 1.792 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2035 proteins (554697 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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