Lachnospiraceae bacterium TWA4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D0QS32|A0A0D0QS32_9FIRM TWA4_scaffold00001 whole genome shotgun sequence OS=Lachnospiraceae bacterium TWA4 OX=1392836 GN=P261_02618 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.2AYY4 pKa = 10.3VDD6 pKa = 3.44QDD8 pKa = 3.15ICIGCGLCAGMEE20 pKa = 4.15PNIFRR25 pKa = 11.84MNNEE29 pKa = 3.43GKK31 pKa = 10.39AEE33 pKa = 4.04TFAEE37 pKa = 4.47GDD39 pKa = 3.74DD40 pKa = 4.27EE41 pKa = 4.67NVQDD45 pKa = 5.41VIDD48 pKa = 4.18SCPVEE53 pKa = 5.0AISEE57 pKa = 4.25EE58 pKa = 4.23

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D0QNM5|A0A0D0QNM5_9FIRM TWA4_scaffold00001 whole genome shotgun sequence OS=Lachnospiraceae bacterium TWA4 OX=1392836 GN=P261_01403 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.76VLQARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2918

0

2918

819781

38

1974

280.9

31.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.447 ± 0.054

1.512 ± 0.019

5.642 ± 0.035

7.545 ± 0.05

4.15 ± 0.033

6.577 ± 0.04

1.759 ± 0.018

7.961 ± 0.043

7.876 ± 0.038

9.112 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.954 ± 0.02

4.837 ± 0.035

3.095 ± 0.024

3.111 ± 0.028

3.765 ± 0.031

6.155 ± 0.039

5.52 ± 0.037

6.791 ± 0.04

0.862 ± 0.015

4.328 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski