Flavobacterium phage vB_FspS_tooticki6-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Muminvirus; Flavobacterium virus Tooticki

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LPE1|A0A6B9LPE1_9CAUD DNA methyltransferase OS=Flavobacterium phage vB_FspS_tooticki6-1 OX=2686279 GN=tooticki61_gp005 PE=4 SV=1
MM1 pKa = 7.67NDD3 pKa = 3.11SIIRR7 pKa = 11.84FKK9 pKa = 11.46NSLNDD14 pKa = 4.29ALNLDD19 pKa = 4.43LNDD22 pKa = 3.76NAILGYY28 pKa = 10.96ANQIVLNPFKK38 pKa = 10.48FYY40 pKa = 8.43TQKK43 pKa = 9.79TPIEE47 pKa = 4.21MIFEE51 pKa = 4.37GEE53 pKa = 4.24YY54 pKa = 9.23TCYY57 pKa = 10.54ISDD60 pKa = 3.39ICGDD64 pKa = 3.7NLQDD68 pKa = 3.64ITEE71 pKa = 4.07HH72 pKa = 6.25TYY74 pKa = 10.49ISEE77 pKa = 4.18NTNGNYY83 pKa = 9.4IEE85 pKa = 4.65FATGVDD91 pKa = 4.24FQRR94 pKa = 11.84KK95 pKa = 8.21LVLIKK100 pKa = 10.45LVNNINPLEE109 pKa = 3.95IWYY112 pKa = 9.41SNPMFITEE120 pKa = 4.31NVNLTTEE127 pKa = 4.42FDD129 pKa = 3.99YY130 pKa = 11.66KK131 pKa = 10.86NVSDD135 pKa = 5.88AYY137 pKa = 8.13MQSVALEE144 pKa = 4.25CFFTRR149 pKa = 11.84SIQEE153 pKa = 4.29SEE155 pKa = 4.23VKK157 pKa = 10.53SYY159 pKa = 10.39VQEE162 pKa = 4.2TGTKK166 pKa = 9.48VSGKK170 pKa = 8.87ATFIEE175 pKa = 3.93MRR177 pKa = 11.84KK178 pKa = 9.81FIFEE182 pKa = 3.94MLDD185 pKa = 3.02NFVYY189 pKa = 10.36RR190 pKa = 11.84RR191 pKa = 11.84LNLILAKK198 pKa = 9.25TQLYY202 pKa = 8.12VQEE205 pKa = 4.43IRR207 pKa = 11.84VTDD210 pKa = 3.75KK211 pKa = 11.14PLLKK215 pKa = 10.87DD216 pKa = 3.68SDD218 pKa = 3.6IQGNQNTFSSEE229 pKa = 3.45FTGAVNYY236 pKa = 10.62NDD238 pKa = 3.69TYY240 pKa = 11.42TRR242 pKa = 11.84TLQIAQPLALVSNYY256 pKa = 9.77PNAIYY261 pKa = 9.83TLDD264 pKa = 4.15TISDD268 pKa = 5.03LIQLVFNHH276 pKa = 7.07DD277 pKa = 3.33VDD279 pKa = 4.02TDD281 pKa = 3.93NEE283 pKa = 4.13DD284 pKa = 3.77LQISLYY290 pKa = 10.02KK291 pKa = 10.7DD292 pKa = 3.56EE293 pKa = 5.43IFISYY298 pKa = 10.65LDD300 pKa = 4.17LIKK303 pKa = 10.69INFTTFEE310 pKa = 3.74QVYY313 pKa = 9.26NFVSNGEE320 pKa = 4.02YY321 pKa = 10.44KK322 pKa = 10.37IIIPANKK329 pKa = 9.46YY330 pKa = 10.6SSILYY335 pKa = 10.22GSLPYY340 pKa = 10.25TEE342 pKa = 4.73LTFTILGGEE351 pKa = 4.35YY352 pKa = 10.64EE353 pKa = 4.11NTEE356 pKa = 4.14YY357 pKa = 11.2NDD359 pKa = 3.59EE360 pKa = 4.16YY361 pKa = 11.52LLNN364 pKa = 4.17

Molecular weight: 42.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LP10|A0A6B9LP10_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_tooticki6-1 OX=2686279 GN=tooticki61_gp038 PE=4 SV=1
MM1 pKa = 7.51SKK3 pKa = 10.45LSKK6 pKa = 10.2RR7 pKa = 11.84KK8 pKa = 10.25SPVLVGNRR16 pKa = 11.84IDD18 pKa = 3.47TTYY21 pKa = 10.81EE22 pKa = 3.51RR23 pKa = 11.84QRR25 pKa = 11.84NITQTAKK32 pKa = 10.36EE33 pKa = 4.08VAEE36 pKa = 4.23RR37 pKa = 11.84TSEE40 pKa = 4.08EE41 pKa = 3.98IKK43 pKa = 10.91NKK45 pKa = 10.09NIRR48 pKa = 11.84YY49 pKa = 9.08DD50 pKa = 3.14IKK52 pKa = 10.88RR53 pKa = 3.47

Molecular weight: 6.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

11722

35

930

180.3

20.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.905 ± 0.35

1.177 ± 0.185

5.648 ± 0.279

7.166 ± 0.321

5.255 ± 0.244

4.718 ± 0.248

1.322 ± 0.178

8.045 ± 0.281

9.469 ± 0.571

8.966 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.122

8.164 ± 0.316

2.423 ± 0.193

3.924 ± 0.318

3.046 ± 0.184

6.501 ± 0.276

6.637 ± 0.367

5.332 ± 0.207

0.896 ± 0.079

4.283 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski