Rhizobium sp. Pop5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0WDB9|K0WDB9_9RHIZ Serine acetyltransferase OS=Rhizobium sp. Pop5 OX=1223565 GN=RCCGEPOP_10216 PE=3 SV=1
MM1 pKa = 7.18TKK3 pKa = 10.5SLIAASLLAIGMASSAFAQSNPDD26 pKa = 4.02PIGPTNGGSTDD37 pKa = 3.82PSSGTYY43 pKa = 10.34SSDD46 pKa = 3.12YY47 pKa = 10.12TNSDD51 pKa = 3.11NGIHH55 pKa = 6.58PVPISPVDD63 pKa = 3.45PTTTQSIGQPQSMEE77 pKa = 4.29CPGMPQQMSGVDD89 pKa = 3.46TRR91 pKa = 11.84GGEE94 pKa = 4.14SGASISEE101 pKa = 3.99ACRR104 pKa = 11.84EE105 pKa = 3.98YY106 pKa = 11.55DD107 pKa = 3.19NN108 pKa = 5.41

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0VYP8|K0VYP8_9RHIZ Cytochrome bd ubiquinol oxidase subunit I OS=Rhizobium sp. Pop5 OX=1223565 GN=RCCGEPOP_16523 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.17GGRR28 pKa = 11.84KK29 pKa = 9.04VIAARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.21RR41 pKa = 11.84LSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7019

0

7019

1824631

17

2433

260.0

28.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.861 ± 0.039

0.81 ± 0.009

5.668 ± 0.025

5.847 ± 0.03

3.969 ± 0.02

8.238 ± 0.028

2.037 ± 0.016

5.791 ± 0.022

3.721 ± 0.023

9.953 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.013

2.864 ± 0.018

4.857 ± 0.022

3.085 ± 0.019

6.755 ± 0.033

5.912 ± 0.026

5.213 ± 0.022

7.238 ± 0.025

1.287 ± 0.012

2.341 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski