Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Oligoflexia; Bdellovibrionales; Bdellovibrionaceae; Bdellovibrio; Bdellovibrio bacteriovorus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3583 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6MR45|Q6MR45_BDEBA Excinuclease ABC subunit C OS=Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100) OX=264462 GN=uvrC PE=4 SV=1
MM1 pKa = 7.63ASCPSCHH8 pKa = 6.2QPVEE12 pKa = 4.22ILDD15 pKa = 3.7KK16 pKa = 11.19HH17 pKa = 6.67LGTLFTCSHH26 pKa = 6.58CNAVFFVDD34 pKa = 3.59WNGQPEE40 pKa = 4.39MAQHH44 pKa = 5.9EE45 pKa = 4.68PEE47 pKa = 5.1PEE49 pKa = 4.0TPAEE53 pKa = 4.29SFSEE57 pKa = 4.21PAQSFQNGTDD67 pKa = 3.81FQGGQDD73 pKa = 3.77FQQPGNDD80 pKa = 3.88FVPPIQDD87 pKa = 3.45FSAPQEE93 pKa = 4.2TFTPDD98 pKa = 2.8NSIPEE103 pKa = 3.92QSYY106 pKa = 10.86QDD108 pKa = 3.14QSYY111 pKa = 11.63SNEE114 pKa = 3.72QQYY117 pKa = 11.3AEE119 pKa = 4.04QAYY122 pKa = 9.76EE123 pKa = 3.99PSQDD127 pKa = 3.96PGMMAPPQDD136 pKa = 3.46NVSYY140 pKa = 10.61AEE142 pKa = 3.95PVEE145 pKa = 4.49VPGEE149 pKa = 4.1VPPMEE154 pKa = 4.28EE155 pKa = 3.49TPYY158 pKa = 11.26DD159 pKa = 4.05FSQTLDD165 pKa = 3.62SVNSQPLNEE174 pKa = 4.5PAPMSSDD181 pKa = 3.02TADD184 pKa = 3.6FSDD187 pKa = 3.63VTDD190 pKa = 4.48FANADD195 pKa = 3.78TAVGPLAYY203 pKa = 9.01TVTIDD208 pKa = 4.17GIEE211 pKa = 4.14SSRR214 pKa = 11.84LLAQLRR220 pKa = 11.84EE221 pKa = 4.02AMTDD225 pKa = 3.39SRR227 pKa = 11.84FGWDD231 pKa = 3.17VADD234 pKa = 4.61LLSHH238 pKa = 6.22VGGGRR243 pKa = 11.84LILHH247 pKa = 7.1GLTPAKK253 pKa = 10.45ASVLINRR260 pKa = 11.84IKK262 pKa = 10.64YY263 pKa = 10.35LPFKK267 pKa = 10.54ISWRR271 pKa = 11.84QDD273 pKa = 3.29VLSGSS278 pKa = 3.67

Molecular weight: 30.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6ML37|Q6ML37_BDEBA Histidine kinase OS=Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100) OX=264462 GN=Bd2184 PE=4 SV=1
MM1 pKa = 7.34QKK3 pKa = 10.1QKK5 pKa = 11.02NIKK8 pKa = 9.58QRR10 pKa = 11.84VTRR13 pKa = 11.84AGTFKK18 pKa = 9.9KK19 pKa = 9.13TKK21 pKa = 10.24AKK23 pKa = 10.43LFLVMRR29 pKa = 11.84ALSRR33 pKa = 11.84GLL35 pKa = 3.39

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3583

0

3583

1168751

30

2828

326.2

36.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.48 ± 0.041

0.96 ± 0.018

5.132 ± 0.031

6.29 ± 0.046

4.484 ± 0.029

7.215 ± 0.047

1.899 ± 0.019

5.603 ± 0.034

6.54 ± 0.048

9.778 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.019

4.139 ± 0.031

4.198 ± 0.029

3.95 ± 0.032

4.768 ± 0.027

6.935 ± 0.039

5.566 ± 0.052

7.108 ± 0.031

1.207 ± 0.017

3.057 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski