Microbacterium sp. TS-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2YQA0|U2YQA0_9MICO ABC transporter ATP-binding protein OS=Microbacterium sp. TS-1 OX=1344956 GN=MTS1_03148 PE=4 SV=1
MM1 pKa = 7.88PSLTSPRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84TLLGVTVALAAALALSSCAAGTSSSEE36 pKa = 4.4DD37 pKa = 3.48GEE39 pKa = 4.31QTLVIGSQMASFPSLDD55 pKa = 3.17TGAITTAGYY64 pKa = 9.71EE65 pKa = 4.25GQRR68 pKa = 11.84LIGNLVYY75 pKa = 10.75EE76 pKa = 4.84GLTKK80 pKa = 10.05RR81 pKa = 11.84DD82 pKa = 3.39VSDD85 pKa = 4.2PNAPAGVAPALASSWEE101 pKa = 4.01VADD104 pKa = 5.68DD105 pKa = 3.91GLSYY109 pKa = 10.92TFTLQDD115 pKa = 2.9GVTFHH120 pKa = 7.87DD121 pKa = 4.3GTDD124 pKa = 3.11WDD126 pKa = 4.45ADD128 pKa = 3.57ALIYY132 pKa = 10.51NINRR136 pKa = 11.84YY137 pKa = 7.95MDD139 pKa = 3.79EE140 pKa = 4.19TDD142 pKa = 3.51PNYY145 pKa = 11.14DD146 pKa = 3.4PVLITYY152 pKa = 7.41YY153 pKa = 10.23TVLNFVDD160 pKa = 4.46SVEE163 pKa = 4.6KK164 pKa = 10.78VDD166 pKa = 5.38DD167 pKa = 3.64MTVTFTLNQPFAFFLADD184 pKa = 4.28LYY186 pKa = 11.36NVYY189 pKa = 9.94FASPTSLDD197 pKa = 3.3EE198 pKa = 5.76DD199 pKa = 4.25GAAGQATNPVGTGPFVFDD217 pKa = 4.69EE218 pKa = 4.32LQQNQSISFVRR229 pKa = 11.84NDD231 pKa = 4.13DD232 pKa = 3.3YY233 pKa = 11.14WGEE236 pKa = 4.2VPQLDD241 pKa = 4.03RR242 pKa = 11.84LTVEE246 pKa = 5.58LIPDD250 pKa = 4.17PSARR254 pKa = 11.84TAALRR259 pKa = 11.84SGRR262 pKa = 11.84VDD264 pKa = 3.34WIEE267 pKa = 3.77AAQPDD272 pKa = 4.54DD273 pKa = 4.01LASLEE278 pKa = 4.33SAGFVATGNAFDD290 pKa = 5.37WEE292 pKa = 4.5WSWQLMTGEE301 pKa = 4.96APFDD305 pKa = 4.89DD306 pKa = 3.36IRR308 pKa = 11.84VRR310 pKa = 11.84QAMNYY315 pKa = 10.07AIDD318 pKa = 3.87RR319 pKa = 11.84EE320 pKa = 4.47AIADD324 pKa = 3.99DD325 pKa = 4.55LLHH328 pKa = 5.92GTAVPAHH335 pKa = 5.78QMFASASLYY344 pKa = 8.79YY345 pKa = 10.52TEE347 pKa = 6.57DD348 pKa = 4.25DD349 pKa = 4.74DD350 pKa = 6.72LYY352 pKa = 11.32DD353 pKa = 4.31YY354 pKa = 11.39DD355 pKa = 4.13PEE357 pKa = 4.2KK358 pKa = 11.11AKK360 pKa = 10.96ALLAEE365 pKa = 4.61AGYY368 pKa = 9.9PDD370 pKa = 4.42GLDD373 pKa = 3.18VTVGYY378 pKa = 8.45ITAGSGAMQAKK389 pKa = 9.49SMNEE393 pKa = 3.52ALQTQLAAVGINVSLEE409 pKa = 3.98PVDD412 pKa = 4.11FSGMYY417 pKa = 10.38AKK419 pKa = 10.53LGEE422 pKa = 4.88GDD424 pKa = 4.24TGWQAANQAFSLEE437 pKa = 4.6QPSSWNGFWFGCDD450 pKa = 3.0GNFFGYY456 pKa = 10.46CNPEE460 pKa = 3.81AEE462 pKa = 4.32ALYY465 pKa = 10.44DD466 pKa = 3.68QALTTVDD473 pKa = 3.66DD474 pKa = 4.3EE475 pKa = 4.39EE476 pKa = 4.49RR477 pKa = 11.84AALLTEE483 pKa = 4.23VGHH486 pKa = 7.13LVTEE490 pKa = 4.48DD491 pKa = 3.64AAWLFVVNDD500 pKa = 3.84TAPRR504 pKa = 11.84AMSSAVTGYY513 pKa = 7.65EE514 pKa = 3.99QPKK517 pKa = 9.7SWWIDD522 pKa = 3.25FTGISMGDD530 pKa = 3.04

Molecular weight: 57.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2XNR2|U2XNR2_9MICO Dipeptide/oligopeptide/nickel ABC transporter permease protein OS=Microbacterium sp. TS-1 OX=1344956 GN=MTS1_02072 PE=4 SV=1
MM1 pKa = 7.7PARR4 pKa = 11.84AVLYY8 pKa = 9.86PRR10 pKa = 11.84VPGLKK15 pKa = 9.65RR16 pKa = 11.84SGDD19 pKa = 3.41RR20 pKa = 11.84RR21 pKa = 11.84SGVIPMSKK29 pKa = 8.84RR30 pKa = 11.84TFQPNNRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84AKK41 pKa = 10.04KK42 pKa = 8.86HH43 pKa = 4.36GFRR46 pKa = 11.84ARR48 pKa = 11.84MRR50 pKa = 11.84TRR52 pKa = 11.84AGRR55 pKa = 11.84AILSARR61 pKa = 11.84RR62 pKa = 11.84GKK64 pKa = 10.71GRR66 pKa = 11.84TEE68 pKa = 4.13LSAA71 pKa = 4.86

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3155

0

3155

1046449

40

2022

331.7

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.084 ± 0.061

0.466 ± 0.009

6.471 ± 0.044

5.511 ± 0.042

3.105 ± 0.027

9.003 ± 0.038

1.896 ± 0.021

4.49 ± 0.032

1.609 ± 0.033

9.961 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.736 ± 0.018

1.81 ± 0.022

5.492 ± 0.033

2.589 ± 0.024

7.684 ± 0.051

5.569 ± 0.029

5.944 ± 0.036

9.138 ± 0.043

1.522 ± 0.022

1.919 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski