Phytomonas sp. isolate EM1

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Phytomonas; unclassified Phytomonas

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6KQH8|W6KQH8_9TRYP Uncharacterized protein OS=Phytomonas sp. isolate EM1 OX=134006 GN=GSEM1_T00001649001 PE=4 SV=1
MM1 pKa = 7.57GCGSSLVYY9 pKa = 9.74TSNKK13 pKa = 8.23GASVGVNSSQTRR25 pKa = 11.84NVTDD29 pKa = 3.89ADD31 pKa = 3.89VNKK34 pKa = 10.48NDD36 pKa = 4.26DD37 pKa = 3.97NAPLSLLPGYY47 pKa = 10.62LDD49 pKa = 5.3DD50 pKa = 6.18DD51 pKa = 3.8FTGPVEE57 pKa = 4.59CYY59 pKa = 8.64TDD61 pKa = 3.24YY62 pKa = 10.91RR63 pKa = 11.84GYY65 pKa = 9.12VTTVSGGASKK75 pKa = 10.5RR76 pKa = 11.84RR77 pKa = 11.84ADD79 pKa = 4.74LLCMWLDD86 pKa = 3.53SALSILNNAGGTFFDD101 pKa = 4.79PEE103 pKa = 4.46SLEE106 pKa = 3.84AWPPDD111 pKa = 3.56LLVV114 pKa = 3.74

Molecular weight: 12.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6KF71|W6KF71_9TRYP Uncharacterized protein OS=Phytomonas sp. isolate EM1 OX=134006 GN=GSEM1_T00004330001 PE=4 SV=1
MM1 pKa = 7.07GRR3 pKa = 11.84WRR5 pKa = 11.84RR6 pKa = 11.84TSSRR10 pKa = 11.84ARR12 pKa = 11.84GRR14 pKa = 11.84SRR16 pKa = 11.84GRR18 pKa = 11.84MGRR21 pKa = 11.84SSPSRR26 pKa = 11.84WAPSRR31 pKa = 11.84RR32 pKa = 11.84FPFPPTRR39 pKa = 11.84MAARR43 pKa = 11.84RR44 pKa = 11.84AISSGSSLGCSTRR57 pKa = 11.84SSSTAARR64 pKa = 11.84ARR66 pKa = 11.84CGSCGINSRR75 pKa = 11.84RR76 pKa = 11.84TSPIAAWSSTRR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84IRR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84SASPSWWSGARR104 pKa = 11.84RR105 pKa = 3.41

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6361

0

6361

3125077

19

6664

491.3

54.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.816 ± 0.028

1.922 ± 0.014

5.061 ± 0.016

6.738 ± 0.03

3.843 ± 0.017

6.01 ± 0.026

2.704 ± 0.013

4.6 ± 0.023

4.722 ± 0.026

9.816 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.011

3.83 ± 0.017

5.313 ± 0.031

3.788 ± 0.017

6.732 ± 0.026

8.514 ± 0.041

5.38 ± 0.019

6.523 ± 0.022

1.078 ± 0.008

2.682 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski