Clostridium oryzae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V4IXE4|A0A1V4IXE4_9CLOT Uncharacterized protein OS=Clostridium oryzae OX=1450648 GN=CLORY_06280 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 5.08NEE4 pKa = 4.55QLLQMDD10 pKa = 4.61AAILLSMVNMKK21 pKa = 10.46LRR23 pKa = 11.84DD24 pKa = 3.89YY25 pKa = 11.05YY26 pKa = 11.55SSLDD30 pKa = 4.02SYY32 pKa = 11.69CDD34 pKa = 3.6DD35 pKa = 4.3ANIDD39 pKa = 3.32IDD41 pKa = 3.96ILINKK46 pKa = 8.99LDD48 pKa = 4.31TIGYY52 pKa = 9.8IYY54 pKa = 10.67DD55 pKa = 3.67PEE57 pKa = 4.51LNKK60 pKa = 10.29FIYY63 pKa = 9.98KK64 pKa = 10.22

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V4IMQ1|A0A1V4IMQ1_9CLOT Fumarate hydratase class II OS=Clostridium oryzae OX=1450648 GN=fumC PE=4 SV=1
MM1 pKa = 7.38SRR3 pKa = 11.84FSSVIALNVNLGKK16 pKa = 9.67QACNFTIFSNFDD28 pKa = 3.09NLAPTRR34 pKa = 11.84NFRR37 pKa = 11.84AKK39 pKa = 10.5NYY41 pKa = 10.26

Molecular weight: 4.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4505

0

4505

1424398

29

2924

316.2

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.102 ± 0.036

1.251 ± 0.012

5.557 ± 0.031

6.693 ± 0.047

4.466 ± 0.028

6.206 ± 0.031

1.495 ± 0.014

9.388 ± 0.043

8.745 ± 0.037

8.794 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.017

6.03 ± 0.031

2.829 ± 0.021

2.656 ± 0.018

3.627 ± 0.022

6.652 ± 0.033

5.031 ± 0.034

6.431 ± 0.027

0.851 ± 0.013

4.451 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski