Rose leaf rosette-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088MTG5|A0A088MTG5_9CLOS HSP 70h OS=Rose leaf rosette-associated virus OX=1543207 PE=3 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84GSRR5 pKa = 11.84YY6 pKa = 9.54VFIWGLFTVWIYY18 pKa = 11.66SDD20 pKa = 3.61TTFPAYY26 pKa = 9.9GICGEE31 pKa = 4.63KK32 pKa = 10.07IPLFRR37 pKa = 11.84VEE39 pKa = 5.02DD40 pKa = 4.25KK41 pKa = 11.52YY42 pKa = 11.72GLTCPITMFGHH53 pKa = 7.12ILTGTKK59 pKa = 10.59DD60 pKa = 3.22PTDD63 pKa = 3.7YY64 pKa = 10.67MDD66 pKa = 4.32LEE68 pKa = 4.17EE69 pKa = 4.65TIEE72 pKa = 4.21HH73 pKa = 6.38KK74 pKa = 10.77CAVGGQSTGPEE85 pKa = 3.74VDD87 pKa = 3.68CNVVNDD93 pKa = 3.86VVSQDD98 pKa = 3.5GATSTVTAEE107 pKa = 4.48HH108 pKa = 6.87EE109 pKa = 4.29MTAA112 pKa = 3.85

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088MGH6|A0A088MGH6_9CLOS Uncharacterized protein OS=Rose leaf rosette-associated virus OX=1543207 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 4.6CAEE5 pKa = 4.48RR6 pKa = 11.84SFLDD10 pKa = 3.35FTIGVVCLFVTGLVIAFFLYY30 pKa = 10.3LYY32 pKa = 7.91KK33 pKa = 10.13TRR35 pKa = 11.84KK36 pKa = 9.29IVGQFAARR44 pKa = 11.84RR45 pKa = 11.84DD46 pKa = 3.69LRR48 pKa = 11.84EE49 pKa = 4.11PQPARR54 pKa = 11.84ALNGGGFAAVVV65 pKa = 3.33

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

5702

64

2781

438.6

48.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.19 ± 0.453

2.403 ± 0.178

5.612 ± 0.504

5.63 ± 0.356

5.489 ± 0.459

6.051 ± 0.384

1.964 ± 0.3

4.472 ± 0.165

5.454 ± 0.466

9.383 ± 0.503

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.316

3.472 ± 0.232

4.227 ± 0.422

1.929 ± 0.189

5.91 ± 0.318

8.892 ± 0.614

5.91 ± 0.606

9.225 ± 0.648

0.859 ± 0.109

3.964 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski