Sellimonas intestinalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Sellimonas

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3054 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3E3JZG1|A0A3E3JZG1_9FIRM Peptidase M23 OS=Sellimonas intestinalis OX=1653434 GN=DW016_14435 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84KK3 pKa = 8.84GKK5 pKa = 10.26KK6 pKa = 10.08VIGAALAGCMTASMFLTACGDD27 pKa = 3.82GNEE30 pKa = 4.48KK31 pKa = 10.43SGKK34 pKa = 7.58TEE36 pKa = 4.39IEE38 pKa = 3.59ILQYY42 pKa = 10.67KK43 pKa = 9.43PEE45 pKa = 4.15AATYY49 pKa = 9.68FDD51 pKa = 3.65QVEE54 pKa = 4.29EE55 pKa = 4.52KK56 pKa = 10.94FNATHH61 pKa = 7.52DD62 pKa = 5.1DD63 pKa = 2.92IHH65 pKa = 6.34LTIDD69 pKa = 3.73SPNDD73 pKa = 2.87ASTIMRR79 pKa = 11.84TRR81 pKa = 11.84FIRR84 pKa = 11.84EE85 pKa = 4.35DD86 pKa = 3.57YY87 pKa = 10.57PDD89 pKa = 4.29IIGIGGDD96 pKa = 3.25INYY99 pKa = 10.09SYY101 pKa = 11.41YY102 pKa = 10.86VDD104 pKa = 4.11AGILADD110 pKa = 3.77VSDD113 pKa = 4.5SEE115 pKa = 4.83VMGEE119 pKa = 4.33IKK121 pKa = 10.5DD122 pKa = 3.77SYY124 pKa = 11.35KK125 pKa = 10.96DD126 pKa = 3.13ILEE129 pKa = 4.25ALEE132 pKa = 3.97IVPTEE137 pKa = 4.01GTFGIPYY144 pKa = 8.54VANAAGILYY153 pKa = 9.69NRR155 pKa = 11.84DD156 pKa = 3.01MFEE159 pKa = 3.74EE160 pKa = 4.54HH161 pKa = 6.39GWEE164 pKa = 5.12IPNTWDD170 pKa = 4.82EE171 pKa = 5.93LMTLCNDD178 pKa = 3.25IQSEE182 pKa = 4.85GILPFYY188 pKa = 10.77FGFKK192 pKa = 9.56DD193 pKa = 2.9TWTCLAPWNSMAVALAPPDD212 pKa = 3.34LCKK215 pKa = 10.23QVNKK219 pKa = 10.47GDD221 pKa = 4.05ANFTDD226 pKa = 4.37SYY228 pKa = 11.39RR229 pKa = 11.84EE230 pKa = 3.79VAEE233 pKa = 5.12KK234 pKa = 10.81YY235 pKa = 10.73LEE237 pKa = 4.06LLNYY241 pKa = 10.11GPEE244 pKa = 4.05DD245 pKa = 3.66PFAYY249 pKa = 9.9GYY251 pKa = 11.37NDD253 pKa = 3.42ACTAFANGEE262 pKa = 4.09SAMYY266 pKa = 9.44PIGSYY271 pKa = 10.32AVPQILSVNPDD282 pKa = 3.23MNIDD286 pKa = 3.84SFVTPASDD294 pKa = 3.16TKK296 pKa = 10.97EE297 pKa = 4.07GNTLNSGVDD306 pKa = 3.29LMFAVTADD314 pKa = 4.06CEE316 pKa = 4.44NKK318 pKa = 8.86EE319 pKa = 3.75AAYY322 pKa = 9.88EE323 pKa = 4.0VLEE326 pKa = 4.27FLYY329 pKa = 10.67EE330 pKa = 4.13DD331 pKa = 4.49EE332 pKa = 5.58NIQAYY337 pKa = 9.68IDD339 pKa = 4.0DD340 pKa = 4.28QNAIPCKK347 pKa = 10.56EE348 pKa = 3.8GDD350 pKa = 3.74FDD352 pKa = 6.05LASQLDD358 pKa = 3.84GMTEE362 pKa = 4.03YY363 pKa = 10.49IEE365 pKa = 4.48AGNMTDD371 pKa = 3.89YY372 pKa = 10.93QDD374 pKa = 3.86HH375 pKa = 6.74YY376 pKa = 9.86YY377 pKa = 9.24PSEE380 pKa = 4.04MAVDD384 pKa = 3.86AQIQTFLINKK394 pKa = 9.55DD395 pKa = 2.41VDD397 pKa = 3.63AFLKK401 pKa = 10.85KK402 pKa = 10.15FDD404 pKa = 4.71SDD406 pKa = 3.08WTRR409 pKa = 11.84YY410 pKa = 9.06NRR412 pKa = 11.84DD413 pKa = 3.08IIRR416 pKa = 11.84EE417 pKa = 3.98VQEE420 pKa = 4.09YY421 pKa = 10.71EE422 pKa = 4.43EE423 pKa = 4.61EE424 pKa = 4.25NGDD427 pKa = 3.53AA428 pKa = 5.2

Molecular weight: 47.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3E3K0I5|A0A3E3K0I5_9FIRM tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Sellimonas intestinalis OX=1653434 GN=mnmG PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3054

0

3054

956250

25

2557

313.1

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.083 ± 0.046

1.496 ± 0.02

5.373 ± 0.034

8.039 ± 0.05

4.205 ± 0.032

7.243 ± 0.04

1.857 ± 0.019

7.379 ± 0.051

6.806 ± 0.035

9.074 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.022

3.844 ± 0.031

3.399 ± 0.026

3.339 ± 0.027

4.955 ± 0.038

5.54 ± 0.037

5.301 ± 0.035

6.823 ± 0.037

0.98 ± 0.017

4.089 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski