Corynebacterium phage Stickynote

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ceetrepovirus; unclassified Ceetrepovirus

Average proteome isoelectric point is 5.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6ETI9|A0A4Y6ETI9_9CAUD Uncharacterized protein OS=Corynebacterium phage Stickynote OX=2588503 GN=2 PE=4 SV=1
MM1 pKa = 6.97TALVNPLSPSMLRR14 pKa = 11.84DD15 pKa = 3.15AAEE18 pKa = 4.2SVVSDD23 pKa = 3.13VLMWAKK29 pKa = 10.53YY30 pKa = 7.79GTEE33 pKa = 3.32ISEE36 pKa = 4.27NVDD39 pKa = 3.5LAIAEE44 pKa = 4.23ILEE47 pKa = 4.19SLAEE51 pKa = 3.98YY52 pKa = 11.04LEE54 pKa = 4.33VEE56 pKa = 5.26PITEE60 pKa = 4.35LDD62 pKa = 3.63DD63 pKa = 3.85VEE65 pKa = 4.99TIVSDD70 pKa = 3.8FCDD73 pKa = 3.45DD74 pKa = 3.46VARR77 pKa = 11.84SLEE80 pKa = 3.92DD81 pKa = 3.1MRR83 pKa = 11.84GNGLATDD90 pKa = 3.57ITDD93 pKa = 3.23GDD95 pKa = 4.01VIYY98 pKa = 10.25TSDD101 pKa = 3.53IITYY105 pKa = 7.54YY106 pKa = 10.33TEE108 pKa = 3.73NTNEE112 pKa = 3.94VEE114 pKa = 4.08EE115 pKa = 4.75ALAEE119 pKa = 4.4CYY121 pKa = 10.51GGLGDD126 pKa = 5.98FDD128 pKa = 5.17TISDD132 pKa = 4.76AISAGVALALEE143 pKa = 4.93NIAEE147 pKa = 4.22SEE149 pKa = 3.87ISGVIDD155 pKa = 5.34AFEE158 pKa = 3.95TWAKK162 pKa = 11.15EE163 pKa = 3.83EE164 pKa = 3.72MTKK167 pKa = 10.97YY168 pKa = 10.3IFAA171 pKa = 5.32

Molecular weight: 18.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6ETK5|A0A4Y6ETK5_9CAUD Uncharacterized protein OS=Corynebacterium phage Stickynote OX=2588503 GN=22 PE=4 SV=1
MM1 pKa = 7.12ARR3 pKa = 11.84KK4 pKa = 9.82RR5 pKa = 11.84GRR7 pKa = 11.84SKK9 pKa = 10.93RR10 pKa = 11.84SLNSNPKK17 pKa = 10.11GKK19 pKa = 10.38GKK21 pKa = 10.64YY22 pKa = 8.25GSKK25 pKa = 9.05WGHH28 pKa = 5.92NFTPKK33 pKa = 10.33NAVARR38 pKa = 11.84KK39 pKa = 9.45LKK41 pKa = 10.57AKK43 pKa = 9.43TYY45 pKa = 9.52GRR47 pKa = 11.84KK48 pKa = 8.98SVKK51 pKa = 9.15TRR53 pKa = 11.84LRR55 pKa = 11.84KK56 pKa = 9.57

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

20193

41

2143

224.4

25.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.993 ± 0.495

0.669 ± 0.098

6.785 ± 0.253

7.854 ± 0.319

3.788 ± 0.199

7.354 ± 0.415

1.783 ± 0.171

5.685 ± 0.163

5.343 ± 0.207

7.904 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.57 ± 0.137

4.348 ± 0.2

4.388 ± 0.146

2.971 ± 0.122

5.982 ± 0.285

6.378 ± 0.268

5.492 ± 0.22

6.577 ± 0.215

1.664 ± 0.108

3.472 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski